Siberian Roe Deer (Capreolus pygargus Pallas, 1771) in Ukraine: Analysis of the Mitochondrial and Nuclear DNA

A molecular-genetic analysis of the nucleotide sequences of the cytochrome b gene (1140 base pairs) of the mitochondrial DNA and 17 microsatellite loci of eight samples of roe deer from the Samara forest of Dnipropetrovsk oblast (Ukraine) was carried out. For comparison, 212 corresponding mtDNA sequences of the Siberian and European roe deer and data on the variability of microsatellite markers in 49 representatives of these species were included in the study. It was noted that all the analyzed mitochondrial sequences of individuals from the Samara forest are characteristic of the Siberian roe Capreolus pygargus Pallas, 1771. Four haplotypes were described, all of which belonged to the haplogroup typical for the western part of the range of C. pygargus. A fragment analysis of the microsatellite loci of nuclear DNA confirmed the identification of the investigated group with the Siberian species

Полиморфизм митохондриальной ДНК европейской косулиCapreolus capreolus (Artiodactyla, Cervidae), юго-западной Украины

EN: Analysis of mtDNA control region (934 b. p.) and cytochrome b gene (1140 b. p.) polymorphism of the 33 roe deer samples from the south-west of Ukraine was carried out. 30 different haplotypes of mtDNA have been described and all of them are related to Capreolus capreolus Linnaeus, 1758. Two well differentiated haplogroups were discovered on the examined territory. The isolated subgroup of related haplotypes was revealed in the Crimea that is likely a result of the long-term geographical isolation of the Crimean roe deer populations. RU: Проведен анализ полиморфизма контрольного региона (934 н. п.) и гена цитохрома b мтДНК (1140 н. п.) 33 образцов косули юго-западной Украины. Описано 30 разных гаплотипов мт ДНК, все они принадлежатCapreolus capreolus Linnaeus, 1758. На исследованной территории обнаружены две хорошо дифференцированные гаплогруппы с обособленной подгруппой близких гаплотипов в Крыму, что скорее всего, является следствием длительной географической изоляции популяции косули Крымского полуострова