9 research outputs found
Blood-Brain Barrier Breakdown in a Single Post-stroke Rodent Brain
Stroke is a major cause of global morbidity and mortality. Middle cerebral artery
occlusion (MCAO) has historically been the most common animal model of simulating
ischemic stroke. The extent of neurological injury after MCAO is typically measured by
cerebral edema, infarct zone, and blood-brain barrier (BBB) permeability. A significant
limitation of these methods is that separate sets of brains must be used for each
measurement. Here we examine an alternative method of measuring cerebral edema,
infarct zone and BBB permeability following MCAO in the same set of brain samples.
Ninety-six rats were randomly divided into three experimental groups. Group 1 (n = 27)
was used for the evaluation of infarct zone and brain edema in rats post-MCAO (n = 17)
vs. sham-operated controls (n = 10). Group 2 (n = 27) was used for the evaluation of
BBB breakdown in rats post-MCAO (n = 15) vs. sham-operated controls (n = 10). In
Group 3 (n = 42), all three parameters were measured in the same set of brain slices in
rats post-MCAO (n = 26) vs. sham-operated controls (n = 16). The effect of Evans blue
on the accuracy of measuring infarct zone by 2,3,5-triphenyltetrazolium chloride (TTC)
staining was determined by measuring infarct zone with and without an applied blue
filter. The effects of various concentrations of TTC (0, 0.05, 0.35, 0.5, 1, and 2%) on the
accuracy of measuring BBB permeability was also assessed. There was an increase in
infarct volume (p < 0.01), brain edema (p < 0.01) and BBB breakdown (p < 0.01) in rats
following MCAO compared to sham-operated controls, whether measured separately
or together in the same set of brain samples. Evans blue had an effect on measuring
infarct volume that was minimized by the application of a blue filter on scanned brain slices. There was no difference in the Evans blue extravasation index for the brain
tissue samples without TTC compared to brain tissue samples incubated in TTC. Our
results demonstrate that measuring cerebral edema, infarct zone and BBB permeability
following MCAO can accurately be measured in the same set of brain samples
Genome sequencing provides insight into the reproductive biology, nutritional mode and ploidy of the fern pathogen M ixia osmundae
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/106716/1/nph12653.pd
A Novel Method for Assessing Cerebral Edema, Infarcted Zone and Blood-Brain Barrier Breakdown in a Single Post-stroke Rodent Brain
Stroke is a major cause of global morbidity and mortality. Middle cerebral artery
occlusion (MCAO) has historically been the most common animal model of simulating
ischemic stroke. The extent of neurological injury after MCAO is typically measured by
cerebral edema, infarct zone, and blood-brain barrier (BBB) permeability. A significant
limitation of these methods is that separate sets of brains must be used for each
measurement. Here we examine an alternative method of measuring cerebral edema,
infarct zone and BBB permeability following MCAO in the same set of brain samples.
Ninety-six rats were randomly divided into three experimental groups. Group 1 (n = 27)
was used for the evaluation of infarct zone and brain edema in rats post-MCAO (n = 17)
vs. sham-operated controls (n = 10). Group 2 (n = 27) was used for the evaluation of
BBB breakdown in rats post-MCAO (n = 15) vs. sham-operated controls (n = 10). In
Group 3 (n = 42), all three parameters were measured in the same set of brain slices in
rats post-MCAO (n = 26) vs. sham-operated controls (n = 16). The effect of Evans blue
on the accuracy of measuring infarct zone by 2,3,5-triphenyltetrazolium chloride (TTC)
staining was determined by measuring infarct zone with and without an applied blue
filter. The effects of various concentrations of TTC (0, 0.05, 0.35, 0.5, 1, and 2%) on the
accuracy of measuring BBB permeability was also assessed. There was an increase in
infarct volume (p < 0.01), brain edema (p < 0.01) and BBB breakdown (p < 0.01) in rats
following MCAO compared to sham-operated controls, whether measured separately
or together in the same set of brain samples. Evans blue had an effect on measuring
infarct volume that was minimized by the application of a blue filter on scanned brain slices. There was no difference in the Evans blue extravasation index for the brain
tissue samples without TTC compared to brain tissue samples incubated in TTC. Our
results demonstrate that measuring cerebral edema, infarct zone and BBB permeability
following MCAO can accurately be measured in the same set of brain samples
Experimental factors affecting PCR-based estimates of microbial species richness and evenness
Pyrosequencing of 16S rRNA gene amplicons for microbial community profiling can, for equivalent costs, yield greater than two orders of magnitude more sensitivity than traditional PCR-cloning and Sanger sequencing. With this increased sensitivity and the ability to analyze multiple samples in parallel, it has become possible to evaluate several technical aspects of PCRbased community structure profiling methods. We tested the effect of amplicon length and primer pair on estimates of species richness number of species and evenness relative abundance of species by assessing the potentially tractable microbial community residing in the termite hindgut. Two regions of the 16S rRNA gene were sequenced from one of two common priming sites, spanning the V1-V2 or V8 regions, using amplicons ranging n length from 352 to 1443 bp. Our results demonstrate that both amplicon length and primer pair markedly influence estimates of richness and evenness. However, estimates of species evenness are consistent among different primer pairs targeting the same region. These results highlight the importance of experimental methodology when comparing diversity estimates across communities