DNA sequences, comprising polymorphic sites at <i>TLR4</i> (A, B) and <i>TLR9</i> (C-E) SNPs.

<p>Reverse strand sequences were determined for all the analyzed amplicons of <i>TLR</i> gene fragments; A. <i>TLR4</i> 896 A>G SNP; B. <i>TLR4</i> 1196 C>T SNP; C-E. <i>TLR9</i> 2848 G>A SNP. AA, CC, GG or GA—genotypes at described <i>TLR</i> SNPs.</p

PCR-RFLP analysis of genotypes at <i>TLR4</i> (A, B) and <i>TLR9</i> (C) SNPs.

<p>RFLP products were resolved in 2% agarose gels, stained with ethidium bromide; A. <i>TLR4</i> 896 A>G SNP; B. <i>TLR4</i> 1196 C>T SNP; C. <i>TLR9</i> 2848 G>A SNP. The numbers on the side of electropherograms indicate the lengths of separated DNA fragments. M—50 bp DNA marker; Ud—undigested PCR product; AA, AG, GG, GA, CC, CT or TT—genotypes at analyzed <i>TLR</i> SNPs.</p

Length of restriction fragments and genotypic profiles.

<p>^a SNP, single nucleotide polymorphism.</p><p>^b bps, base pairs.</p><p>Length of restriction fragments and genotypic profiles.</p

Primer sequences, annealing temperatures and amplicon lengths, obtained in multiplex nested PCR assay for SNPs in the <i>TLR4</i> and <i>TLR9</i> genes.

<p>^a No., number.</p><p>^b SNP, single nucleotide polymorphism.</p><p>^c bps, base pairs.</p><p>Primer sequences, annealing temperatures and amplicon lengths, obtained in multiplex nested PCR assay for SNPs in the <i>TLR4</i> and <i>TLR9</i> genes.</p

Visualization of PCR-RFLP products for <i>TLR3</i> SNP (A) and <i>TLR7</i> SNP (B) genotyping.

<p>Gel image: A) rs3775290: 1. wild type CC, 2. heterozygous CT, 3. homozygous recessive TT; rs3775291: 4. wild type CC, 5. heterozygous CT, 6. homozygous recessive TT; rs3775296: 7. wild type CC, 8. heterozygous CA, 9. homozygous recessive AA. B) rs179008: 1. wild type AA, 2. heterozygous AT, 3. homozygous recessive TT; rs5741880: 4. wild type GG, 5. heterozygous GT, 6. homozygous recessive TT. Alignment markers (15 bp, 1 kbp).</p

The distribution of allele frequencies of TLR SNPs in infants with and without HCMV infection.

<p>The distribution of allele frequencies of TLR SNPs in infants with and without HCMV infection.</p

Selected single-nucleotide polymorphisms in the <i>TLR3</i> and <i>TLR7</i> genes.

<p>Selected single-nucleotide polymorphisms in the <i>TLR3</i> and <i>TLR7</i> genes.</p

Frequency and distribution of <i>TLR</i> genotypes and their association with HCMV infection.

<p>Frequency and distribution of <i>TLR</i> genotypes and their association with HCMV infection.</p

Restriction enzymes and length of the restriction fragments.

<p>Restriction enzymes and length of the restriction fragments.</p

Demographic and clinical characteristics of study subjects with HCMV infection.

<p>Demographic and clinical characteristics of study subjects with HCMV infection.</p