Effect of APAP and SLM on RIP-3 expression.

<p>The liver homogenate was separated by SDS-ELFO, immunoblotted and the proteins of interest were detected using specific antibody.</p

Effect of APAP and SLM on lipid peroxidation in the liver.

<p>Data are given as mean ± SEM, n = 7. *p < 0.05 APAP vs control; ^#p < 0.05, ^##p < 0.01 APAP + SLM vs APAP.</p

Effect of APAP and SLM on reactive oxygen species production from submitochondrial particles in vitro.

<p>(A) ROS production from NADH -/+ AA; (B) ROS production from succinate -/+ AA. Results are expressed as a percent of basal levels (without substrate). Data are presented as a mean ± SEM, n = 7. *p < 0.05, ***p < 0.001 APAP vs control (succinate + AA); ^† p < 0.05 APAP + SLM vs APAP (only succinate); ^#p < 0.05, ^## p < 0.01 APAP + SLM vs APAP (succinate + AA).</p

Silymarin prevents acetaminophen-induced hepatotoxicity in mice - Fig 3

<p><b>Effect of APAP and SLM on CYP2E1 activity in vivo (A), mRNA expression (B), protein content (C) and CYPE1 activity in vitro (D, E).</b> Results represent mean ± SEM, n = 7. *p < 0.05, **p < 0.01, ***p < 0.001 APAP vs control. ^#p < 0.05, ^##p < 0.01 APAP + SLM vs APAP.</p

Effect of APAP and SLM on neutrophil infiltration.

<p>Tissue sections were stained with hematoxylin and eosin, black arrows indicate infiltrating neutrophiles. Original magnification x 600.</p

Effect of APAP and SLM on oxidative stress in the liver.

<p>(A) GSH content in liver homogenate; (B) GSSG content in liver homogenate; (C) HO-1 mRNA expression; (D) p-JNK and t-JNK protein expression in liver homogenate. Results are expressed as mean ± SEM, n = 7. *p < 0.05, **p < 0.01, ***p < 0.001 APAP group vs control; ^#p < 0.05, ^## p < 0.01, ^### p < 0.001 APAP + SLM group vs APAP group.</p

Effect of APAP and SLM on mRNA expression of selected inflammatory markers.

<p>(A) Ccl2; (B) Ccr2; (C) Ccl3; (D) TNFα; (E) IL 1β; (F) IL 12; (G) iNOS. Data are expressed as a fold change related to the untreated (control) group. Values are given as a mean ± SEM, n = 7. *p < 0.05, **p < 0.01, ***p < 0.001 APAP vs control; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 APAP+SLM vs APAP.</p