Effects of Different Pre-processing Methods on

Using agricultural wastes for anaerobic fermentation to produce biogas can not only realize the resource utilization of the wastes, but also prevent the environmental issues caused by straw burning. Sugarcane leaves contain waxy layer, which will cause problems such as difficulty in degradation, long-time fermentation and low biogas production. This paper studies the effects on three pre-processing methods of adding rush rot agent, natural retting for 7 days and water moisture for 24 hours of dry anaerobic fermentation of sugarcane leaves and pig manure. The results show that natural retting for 7 days has the advantage of daily biogas production when the fermentation period is less than 20 days, but this method is not obviously different from that of water moisture for 24 hours. When taking total biogas production volume and volume loading rate as the indicators, water moisture for 24 hours is the best pre-processing method for dry anaerobic fermentation of sugarcane leaves and pig manure when the fermentation period is more than 20 days

A Dynamic Analysis of the

With the rotary cutting system of king grass shredder as the research object, this paper established finite element models for rotating shaft, rotating shaft-belt pulley, rotating shaft-rotary cutting part and rotary cutting system and analyzed the influences of belt pulley and rotary cutting part on the dynamic characteristics of rotary cutting system. The results showed that the belt pulley and rotary cutting part had a great influence on the second order critical speed of rotary cutting system, and the rotary cutting part had a greater influence on the critical speed of first order forward precession than the belt pulley. Meanwhile, the critical speed of rotary cutting system that conformed to facts was calculated. There was a big difference between its first order and second order critical speeds, but the critical speed of first order backward precession was lower. Finally, it was found after analysis that the natural frequency of rotary cutting system was lower than the vibration frequency induced by belt drive, so the shredder can run safely

Data_Sheet_1_Differential proteomic of plasma provides a new perspective on scientific diagnosis and drug screening for dampness heat diarrhea in calves.doc

Dampness heat diarrhea (DHD) is one of the most common syndromes of calf diarrhea. Its complex etiology and lack of objective diagnostic criteria bring great challenges to the diagnosis and treatment of this disease. This study aims to screen some prospective diagnostic biomarkers or therapeutic targets for calves with DHD by investigating the differential protein profiles of plasma between DHD calves and clinically healthy calves by mass spectrometry-based proteomic. A total of 120 DHD calves and 90 clinically healthy calves were divided into two groups randomly, 30 DHD calves and 30 clinically healthy calves in the test group, and 90 DHD calves and 60 clinically healthy calves in the validation group. In the test group, a total of 52 proteins were differentially expressed between calves with DHD and clinically healthy calves, 13 proteins were significantly increased and 39 proteins were significantly decreased. The differentially expressed proteins were associated with the intestinal immune network of IgA production, caffeine metabolism, purine metabolism, and PI3K signaling pathway. In the validation group, 13 proteins were selected from 52 differential expression proteins for parallel reaction monitoring validation to verify their associations with DHD calves. The targeted proteomic results showed that fibronectin precursor (FN1) and apolipoprotein C-IV precursor (APOC4) were significantly associated with DHD in calves, and they were downregulated in sick calves. In conclusion, the differential expression of plasma proteins was associated with DHD pathogenesis in calves, and the FN1 and APOC4 might be the potential clinical biomarkers for diagnosis of DHD in calves, and the intestinal immune network of IgA production, caffeine metabolism, purine metabolism, and PI3K signaling pathway are the candidate targets to treat DHD in calves. Our finding provides a reference for further investigating the pathogenesis, developing techniques of diagnosis, and screening treatment drugs for DHD in calves.</p

Data_Sheet_2_Differential proteomic of plasma provides a new perspective on scientific diagnosis and drug screening for dampness heat diarrhea in calves.docx

Dampness heat diarrhea (DHD) is one of the most common syndromes of calf diarrhea. Its complex etiology and lack of objective diagnostic criteria bring great challenges to the diagnosis and treatment of this disease. This study aims to screen some prospective diagnostic biomarkers or therapeutic targets for calves with DHD by investigating the differential protein profiles of plasma between DHD calves and clinically healthy calves by mass spectrometry-based proteomic. A total of 120 DHD calves and 90 clinically healthy calves were divided into two groups randomly, 30 DHD calves and 30 clinically healthy calves in the test group, and 90 DHD calves and 60 clinically healthy calves in the validation group. In the test group, a total of 52 proteins were differentially expressed between calves with DHD and clinically healthy calves, 13 proteins were significantly increased and 39 proteins were significantly decreased. The differentially expressed proteins were associated with the intestinal immune network of IgA production, caffeine metabolism, purine metabolism, and PI3K signaling pathway. In the validation group, 13 proteins were selected from 52 differential expression proteins for parallel reaction monitoring validation to verify their associations with DHD calves. The targeted proteomic results showed that fibronectin precursor (FN1) and apolipoprotein C-IV precursor (APOC4) were significantly associated with DHD in calves, and they were downregulated in sick calves. In conclusion, the differential expression of plasma proteins was associated with DHD pathogenesis in calves, and the FN1 and APOC4 might be the potential clinical biomarkers for diagnosis of DHD in calves, and the intestinal immune network of IgA production, caffeine metabolism, purine metabolism, and PI3K signaling pathway are the candidate targets to treat DHD in calves. Our finding provides a reference for further investigating the pathogenesis, developing techniques of diagnosis, and screening treatment drugs for DHD in calves.</p

Additional file 1 of Prevalence and molecular characterization of extended-spectrum β–lactamase—producing Escherichia coli isolates from dairy cattle with endometritis in Gansu Province, China

Additional file 1