Programming Thermoresponsiveness of NanoVelcro Substrates Enables Effective Purification of Circulating Tumor Cells in Lung Cancer Patients

[[sponsorship]]化學研究所[[note]]已出版;[SCI];有審查制度;具代表性[[note]]http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Drexel&SrcApp=hagerty_opac&KeyRecord=1936-0851&DestApp=JCR&RQ=IF_CAT_BOXPLO

Increased calcium and decreased magnesium and citrate concentrations of submandibular/sublingual saliva in sialolithiasis

Objective: The purpose of the present study was to investigate the electrolyte alterations of submandibular/sublingual saliva in submandibular sialolithiasis patients compared with saliva from healthy controls. Design: Submandibular/sublingual saliva was collected from 10 submandibular sialolithiasis patients and from 10 sex- and age-matched healthy controls. Ion chromatography was performed to determine the concentrations of inorganic cations (potassium, calcium, magnesium, and sodium), inorganic anions (chloride, phosphate, nitrate, and sulphate) and organic anions (thiocyanate, lactate, acetate, and citrate). Results: The calcium concentration was significantly higher in sialolithiasis patients than in controls (P < 0.05). In contrast, the levels of magnesium and citrate in the saliva of sialolithiasis patients were significantly reduced compared to the values obtained in controls (P < 0.05). No significant differences were detected among other ions. Conclusions: Our findings indicate that saliva electrolyte composition of sialolithiasis patients is substantially altered with respect to crystallisation mechanisms. Increased calcium ion as a crystallisation substance and decreased magnesium and citrate ions as crystallisation inhibitors may be involved in the etiopathology of calculi formation. © 2009 Elsevier Ltd. All rights reserved.link_to_subscribed_fulltex

The preoperative platelet–lymphocyte ratio neutrophil–lymphocyte ratio: which is better as a prognostic factor in oral squamous cell carcinoma?

Objective: Recent studies have shown that the presence of systemic inflammation and platelet status correlate with poor survival in various cancers. The aim of this study was to evaluate the prognostic value of the preoperative platelet–lymphocyte ratio (PLR) and the neutrophil–lymphocyte ratio (NLR) in patients with oral squamous cell carcinoma (OSCC) undergoing surgery. Methods: In this study, 306 patients with OSCC who had surgery were enrolled. The optimal cutoff value of PLR and NLR was determined by receiver operating characteristic (ROC) curve analysis. The prognostic significance of both markers was determined by uni- and multivariate analysis. Results: The results showed that high NLR and PLR were classified using a cutoff value of 2.7 and 135, respectively, based on ROC curve analysis. Only PLR was associated with decreased disease-free survival [hazard ratio (HR) = 2.237; 95% confidence interval (CI): 1.401–3.571; p = 0.001] and overall survival [HR = 2.022; 95% CI: 1.266–3.228; p = 0.003] by both uni- and multivariate analysis. Conclusion: The preoperative PLR is superior to NLR as an independent indicator in predicting disease-free survival and overall survival in patients who undergo oral cancer resection for OSCC

Nanostructured Substrates for Detection and Characterization of Circulating Rare Cells: From Materials Research to Clinical Applications

Circulating rare cells in the blood are of great significance for both materials research and clinical applications. For example, circulating tumor cells (CTCs) have been demonstrated as useful biomarkers for "liquid biopsy" of the tumor. Circulating fetal nucleated cells (CFNCs) have shown potential in noninvasive prenatal diagnostics. However, it is technically challenging to detect and isolate circulating rare cells due to their extremely low abundance compared to hematologic cells. Nanostructured substrates offer a unique solution to address these challenges by providing local topographic interactions to strengthen cell adhesion and large surface areas for grafting capture agents, resulting in improved cell capture efficiency, purity, sensitivity, and reproducibility. In addition, rare-cell retrieval strategies, including stimulus-responsiveness and additive reagent-triggered release on different nanostructured substrates, allow for on-demand retrieval of the captured CTCs/CFNCs with high cell viability and molecular integrity. Several nanostructured substrate-enabled CTC/CFNC assays are observed maturing from enumeration and subclassification to molecular analyses. These can one day become powerful tools in disease diagnosis, prognostic prediction, and dynamic monitoring of therapeutic response-paving the way for personalized medical care

Nanostructured Substrates for Detection and Characterization of Circulating Rare Cells: From Materials Research to Clinical Applications

Circulating rare cells in the blood are of great significance for both materials research and clinical applications. For example, circulating tumor cells (CTCs) have been demonstrated as useful biomarkers for "liquid biopsy" of the tumor. Circulating fetal nucleated cells (CFNCs) have shown potential in noninvasive prenatal diagnostics. However, it is technically challenging to detect and isolate circulating rare cells due to their extremely low abundance compared to hematologic cells. Nanostructured substrates offer a unique solution to address these challenges by providing local topographic interactions to strengthen cell adhesion and large surface areas for grafting capture agents, resulting in improved cell capture efficiency, purity, sensitivity, and reproducibility. In addition, rare-cell retrieval strategies, including stimulus-responsiveness and additive reagent-triggered release on different nanostructured substrates, allow for on-demand retrieval of the captured CTCs/CFNCs with high cell viability and molecular integrity. Several nanostructured substrate-enabled CTC/CFNC assays are observed maturing from enumeration and subclassification to molecular analyses. These can one day become powerful tools in disease diagnosis, prognostic prediction, and dynamic monitoring of therapeutic response-paving the way for personalized medical care

Canopy Chlorophyll Density Based Index for Estimating Nitrogen Status and Predicting Grain Yield in Rice

Canopy chlorophyll density (Chl) has a pivotal role in diagnosing crop growth and nutrition status. The purpose of this study was to develop Chl based models for estimating N status and predicting grain yield of rice (Oryza sativa L.) with Leaf area index (LAI) and Chlorophyll concentration of the upper leaves. Six field experiments were conducted in Jiangsu Province of East China during 2007, 2008, 2009, 2013, and 2014. Different N rates were applied to generate contrasting conditions of N availability in six Japonica cultivars (9915, 27123, Wuxiangjing 14, Wuyunjing 19, Yongyou 8, and Wuyunjing 24) and two Indica cultivars (Liangyoupei 9, YLiangyou 1). The SPAD values of the four uppermost leaves and LAI were measured from tillering to flowering growth stages. Two N indicators, leaf N accumulation (LNA) and plant N accumulation (PNA) were measured. The LAI estimated by LAI-2000 and LI-3050C were compared and calibrated with a conversion equation. A linear regression analysis showed significant relationships between Chl value and N indicators, the equations were as follows: PNA = (0.092 × Chl) − 1.179 (R2 = 0.94, P &lt; 0.001, relative root mean square error (RRMSE) = 0.196), LNA = (0.052 × Chl) − 0.269 (R2 = 0.93, P &lt; 0.001, RRMSE = 0.185). Standardized method was used to quantity the correlation between Chl value and grain yield, normalized yield = (0.601 × normalized Chl) + 0.400 (R2 = 0.81, P &lt; 0.001, RRMSE = 0.078). Independent experimental data also validated the use of Chl value to accurately estimate rice N status and predict grain yield

New insights into the correlations between circulating tumor cells and target organ metastasis

Abstract Organ-specific metastasis is the primary cause of cancer patient death. The distant metastasis of tumor cells to specific organs depends on both the intrinsic characteristics of the tumor cells and extrinsic factors in their microenvironment. During an intermediate stage of metastasis, circulating tumor cells (CTCs) are released into the bloodstream from primary and metastatic tumors. CTCs harboring aggressive or metastatic features can extravasate to remote sites for continuous colonizing growth, leading to further lesions. In the past decade, numerous studies demonstrated that CTCs exhibited huge clinical value including predicting distant metastasis, assessing prognosis and monitoring treatment response et al. Furthermore, increasingly numerous experiments are dedicated to identifying the key molecules on or inside CTCs and exploring how they mediate CTC-related organ-specific metastasis. Based on the above molecules, more and more inhibitors are being developed to target CTCs and being utilized to completely clean CTCs, which should provide promising prospects to administer advanced tumor. Recently, the application of various nanomaterials and microfluidic technologies in CTCs enrichment technology has assisted to improve our deep insights into the phenotypic characteristics and biological functions of CTCs as a potential therapy target, which may pave the way for us to make practical clinical strategies. In the present review, we mainly focus on the role of CTCs being involved in targeted organ metastasis, especially the latest molecular mechanism research and clinical intervention strategies related to CTCs