Long-Term Variability of Aerosol Concentrations and Optical Properties over the Indo-Gangetic Plain in South Asia

Emissions of atmospheric pollutants are rapidly increasing over South Asia. A greater understanding of seasonal variability in aerosol concentrations over South Asia is a scientific challenge and has consequences due to a lack of monitoring and modelling of air pollutants. Therefore, this study investigates aerosol patterns and trends over some major cities in the Indo-Gangetic Plain of the South Asia, i.e., Islamabad, Lahore, Delhi, and Dhaka, by using simulations from the Modern -Era Retrospective Analysis for Research and Applications, version 2 (MERRA-2) model and satellite measurements (Moderate Resolution Imaging Spectroradiometer, (MODIS)) from 2000 to 2020. The results show that seasonal MODIS–aerosol optical depth (AOD) during 2000−2020 in Lahore is 0.5, 0.52, 0.92, and 0.71, while in Islamabad 0.25, 0.32, 0.45, and 0.38, in Delhi 0.68, 0.6, 1.0, and 0.77, and in Dhaka 0.79, 0.75, 0.78 and 0.55 values are observed during different seasons, i.e., winter, spring, summer, and autumn, respectively. The analysis reveals a significant increase in aerosol concentrations by 25%, 24%, 19%, and 14%, and maximum AOD increased by 15%, 14%, 19%, and 22% during the winter of the last decade (2011–2020) over Islamabad, Lahore, Delhi, and Dhaka, respectively. In contrast, AOD values decreased during spring by −5%, −12%, and −5 over Islamabad, Lahore, and Delhi, respectively. In Dhaka, AOD shows an increasing trend for all seasons. Thus, this study provides the aerosol spatial and temporal variations over the South Asian region and would help policymakers to strategize suitable mitigation measurements.This research was funded by the Higher Education Commission (HEC) Pakistan PBAIRP grant # 22-17 for funding to complete this project

Antidiabetic Activities of an LC/MS Fingerprinted Aqueous Extract of Fagonia cretica L. in Preclinical Models

Diabetes mellitus is a chronic disease and one of the most important public health challenges facing mankind. Fagonia cretica is a medicinal plant used widely in the Punjab in Pakistan. A recent survey has demonstrated that traditional healers and herbalists frequently use this plant to treat diabetes. In the current study, the traditional medicine was prepared as a tea, and the profile of the main metabolites present in the traditional medicine was analysed via LC/MS/MS. The extract was shown to contain a number of phenolic glycosides including quercetin-3-O-rutinoside, kaempferol-3-O-rutinoside, kaempferol-3-O-glycoside, kaempferol-3(6′-malonylglucoside), isorhamnetin-3-O-rutinoside, and isorhamnetin 3-(6″-malonylglucoside) in addition to two unidentified sulphonated saponins. The traditional medicine inhibits α-glucosidase in vitro with an IC50 of 4.62 µg/mL. The hypoglycaemic effect of the traditional medicine was evaluated in normoglycaemic and streptozotocin-treated diabetic rats, using glibenclamide as an internal control. The preparation (250 or 500 mg/kg body weight) was administered once a day for 21 consecutive days. The dose of 500 mg/kg was effective in the management of the disease, causing a 45 % decrease in the plasma glucose level at the end of the experimental period. Histological analysis of pancreatic sections confirmed that streptozotocin/nictotinamide treatment caused destruction of pancreatic islet cells, while pancreatic sections from the treatment groups showed that both the extract and glibenclamide partially prevented this deterioration. The mechanism of this protective effect is unclear. However, such a finding suggests that ingestion of the tea could confer additional benefits and should be investigated further

Mutations in PGRN gene associated with the risk of psoriasis in Pakistan: a case control study

Abstract Background Psoriasis is a chronic, autoimmune, papulosquamous skin disorder, characterized by the formation of drop-like papules and silvery-white plaques surrounded by reddened or inflamed skin, existing predominantly on the scalp, knees and elbows. The characteristic inflammation and hyperproliferation of keratinocytes in psoriasis is regulated by progranulin (PGRN), which suppresses the expression and release of inflammatory cytokines, such as TNF-α. Methodology In this study mutation analysis of the PGRN gene was performed by extracting the genomic DNA from blood samples of 171 diagnosed psoriasis patients and controls through standard salting-out method, followed by amplification and sequencing of the targeted region of exon 5–7 of PGRN gene. Results Three single nucleotide polymorphisms, rs25646, rs850713 and a novel point mutation 805A/G were identified in the PGRN gene with significant association with the disease. The variant alleles of the polymorphisms were significantly distributed among cases and controls, and statistical analysis suggested that the mutant genotypes conferred a higher risk of psoriasis development and progression. Multi-SNP haplotype analysis indicated that the CAA (OR = 8.085, 95% CI = 5.16–12.66) and the CAG (OR = 3.204, 95% CI = 1.97–5.21) haplotypes were significantly associated with psoriasis pathogenesis. Conclusions These findings demonstrate that polymorphisms in PGRN might act as potential molecular targets for early diagnosis of psoriasis in susceptible individuals