Demographic Factors Associated with Acceptance of COVID-19 Vaccination: An Online Survey-Based Study from Hyderabad Sindh

Background: COVID19 is a major threat to global health. World health organization has proposed use of vaccines against COVID19 to end the pandemic. Due to low literacy rate in many developing countries and disinformation, the population is reluctant for COVID19 vaccination. To explore the demographic factors associated with acceptance of COVID19 vaccination, we set up an online cross-sectional study. Methods: The online survey-based cross-sectional study was conducted to understand the demographic factors of residents of Hyderabad city associated with vaccine acceptance. The questionnaire was divided into sections to get sociodemographic characters and other information related with willingness of vaccination. This questionnaire was sent to 300 people from which 205 people participated. Results: The result showed that 28.8% of the participants were not sure to get vaccination of COVID-19, while 41.5% participants were willing to get vaccine if available; rest of the people (29.8%) were not willing be vaccinated at all. The main reason behind this denial was due to belief on conspiracies against COVID-19 vaccine. Many participants either denied (42%) for any disinformation or were not sure (12%) on this account. Conclusion: The study was based on demographic factors of residents of Hyderabad associated with acceptance of COVID-19 vaccine. Among the studied population only 41.5% showed willingness to get vaccinated which indicate the necessity to effectively educate the population about COVID-19 vaccines

Phylogenetic group B2 expressed significant biofilm formation among drug-resistant uropathogenic Escherichia coli

Biofilm is an important virulent marker attributed to the development of urinary tract infections (UTIs) by uropathogenic E. coli (UPEC). Drug-resistant and biofilm-producing UPEC are highly problematic causing catheter-associated or recurrent UTIs with significant morbidity and mortality. The aim of the current study was to investigate the prevalence of biofilm formation and phylogenetic groups in drug-resistant UPEC to predict their ability to cause disease. This prospective study was conducted at the Department of Microbiology, University of Karachi from January to June 2019. A total of 50 highly drug-resistant UPEC were selected for this study. UPEC isolates were screened to form biofilm by Congo-red agar (CRA) and microtiter plate (MTP) technique. The representative biofilm-producing isolates were analysed by scanning electron microscopy (SEM) monitoring. Phylogenetic analysis was done by PCR method based on two preserved genes; chuA, yjaA and TspE4-C2 DNA fragment. On CRA 34 (68%) UPEC were slime producers, while on MTP 20 (40%) were strong biofilm producers, 19 (38%) moderate and 11 (22%) were low to negligible biofilm producers. Molecular typing confirmed that phylogenetic group B2 was prevalent in drug resistant UPEC strains. Pathogenic strains belonged to phylogenetic group B2 and D were found to have greater biofilm forming ability as compare to non-pathogenic commensal strains that belonged to phylogenetic group A. Our results indicate that biofilm formation vary in drug resistant UPEC belonged to different phylogenetic groups. This study indicates possible link between in vitro biofilm formation and phylogenetic groups of UPEC, therefore this knowledge might be helpful to predict the pathogenic potential of UPEC and help design strategies for controlling UTIs

Cost effectiveness of responsive stimulation and nutrition interventions on early child development outcomes in Pakistan

Early childhood programs are heralded as a way to improve children’s health and educational outcomes. However, few studies in developing countries calculate the effectiveness of quality early childhood interventions. Even fewer estimate the associated costs of such interventions. The study here looks at the costs and effectiveness of a clusterrandomized effectiveness trial on children from birth to 24 months in rural Sindh, Pakistan. Responsive stimulation and/or enhanced nutrition interventions were integrated in the Lady HealthWorker program in Pakistan. Outcomes suggest that childrenwho receive responsive stimulation had significantly better development outcomes at 24months than those who only received enhanced nutrition intervention. A cost-effectiveness analysis of the results verifies that early childhood interventions that include responsive stimulation aremore cost effective than a nutrition intervention alone in promoting children’s early development. Costs of a responsive stimulation intervention integrated in an existing community-based service providing basic health and nutrition care is approximately US$4 per month per child. We discuss these findings and make recommendations about scaling up and costs for future early child development programs

Comparative Analysis of Urban Development Trends of Beijing and Karachi Metropolitan Areas

Urban sustainability has become a significant challenge globally due to rapidly growing urbanization and industrialization. Hence, this study was conducted to examine the existing urban development trends of metropolitan cities (i.e., Beijing and Karachi) of China and Pakistan in the context of urban sustainability. The development progress in both cities was examined through urban sustainability indices individually and compared with each other. An index system considering 36 indicators was developed based on social, economic, and environmental aspects. The six years (2012–2017) of data used in this study, were obtained from various governmental websites. Furthermore, the Urban Sustainability Index (USI) was evaluated using the statistical standard equal-weighted method. Results revealed that Beijing’s developmental progress is much better than Karachi in terms of socio-economic and environmental development, but there is still a need for improvement. While Karachi faces problems in all three sectors, each indicator of these sectors requires considerable improvement for attaining the top level of the Urban Sustainability Index. However, this study recommends that much attention is needed in the case of Karachi city, whereas Beijing also needs quality advancement in urban development

Assessment of pre and post Neonatal Resuscitation program (NRP) training in a neonatal intensive care unit of Pakistan

Objective: To determine the pre and post Neonatal Resuscitation Program (NRP) training score in a neonatal unit. Study Design: Quasi experimental study Place and duration of study: NRP training was conducted at Neonatal unit of CMH hospital, Rawalpindi on 20 September 2022. Methodology: A total of 111 participants were included through non-probability purposive sampling. Participants including doctors, specialists, nurses etc. who attended the NRP training of any age and both gender were included. It was a two days training procedure. All the participants were given pre training questionnaire and post training questionnaire. Pre and post evaluation of the questionnaires was done. SPSS v-26 was used for data entry and analysis. Descriptive and inferential statistics was applied. Results: A total of 111 participants were included with the mean age of 36.4±11.3 years. Gender distribution of study population showed 37% (n=41) male and 63% (n=70) females. The study population comprised on consultants (38.6%), nurses (25.4%), trainees (28.1%) and few medical officers (5.3%). The mean score percent of pre and post training assessment was calculated to be 61.72±19.65% and 85.8±10.9% respectively, which showed that post training score was improved after training with statistically significant findings (p<0.0001). Conclusion: It is concluded that the neonatal resuscitation program has proven to be effective in improving the knowledge and skills regarding neonatal resuscitation practice

Polyelectrolyte Multicomponent Colloidosomes Loaded with Nisin Z for Enhanced Antimicrobial Activity against Foodborne Resistant Pathogens

Food grade micro- or nano-carrier systems (NCS) are being developed to improve the controlled release of antimicrobial agents. To augment the stability of liposomal NCS and to overcome the limitations associated with the use of free bacteriocin (nisin) in the food system, multi-component colloidosomes (MCCS) were developed by electrostatic interactions between anionic alginate and cationic chitosan (multilayer) around phospholipids based liposomes (core). Zeta-sizer results revealed the average diameter of 145 ± 2 nm, 596 ± 3 nm, and 643 ± 5 nm for nano-liposome (NL), chitosomes (chitosan coated NL) and MCCS, respectively. Zeta potential values of NCS varied from −4.37 ± 0.16 mV to 33.3 ± 6 mV, thus both chitosomes (CS) and MCCS were positively charged. Microstructure analysis by scanning electron microscope (SEM) revealed relatively higher size of MCCS with smooth and round morphology. TGA and DSC based experiments revealed that MCCS were thermally more stable than uncoated liposomes. Encapsulation efficiency of nisin in MCCS was observed to be 82.9 ± 4.1%, which was significantly higher than NL (56.5 ± 2.5%). FTIR analyses confirmed the cross-linking between sodium alginate and chitosan layer. Both qualitative (growth kinetics) and quantitative (colony forming unit) antimicrobial assays revealed that nisin loaded MCCS have superior potential to control resistant foodborne pathogens including Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecalis, (5.8, 5.4, and 6.1 Log CFUmL−1 reduction, respectively) as compared to free nisin, loaded NL or CS. Controlled release kinetics data fitted with Korsmeyer–Peppas model suggested that nisin release from MCCS followed Fickian diffusion. Cytotoxic studies on human blood cells and HepG2 cell lines revealed hemocompatibility and non-toxicity of MCCS. Thus, due to enhanced controlled release, stability and biocompatibility; these multi-component colloidosomes can be useful for incorporating antimicrobial agents into functional foods, beverages and pharmaceutical products to combat pathogenic and spoilage bacteria