Formulasi Pupuk Hayati Serbuk Menggunakan Bakteri Pelarut Fosfat Indigenus Asal Tanah Gambut Riau Dalam Berbagai Bahan Pembawa

Peat soil has been known for its low content in phosphor (P). On the other hand, P solubilizing process is naturally slow. Inoculation of phosphate solubilizing bacteria (PSB) could increase this process. Therefore, the exploration of bacteria with such potential for use as biofertilizer agents is needed. Quality of biofertilizer depends on the viability and amount of bacteria contained, it is also influenced by type of carrier material. This research was aimed to find the appropriate carrier materials in production of biofertilizer which were contained PSB agent and to determine the quality of biofertilizer produced at a certain storage time. As many as 4 selected PSB isolates (BB_UB6, BB_K9, BB_K2, and BB_HS13) were used to produce 3 starter combinations. The starters were prepared by growing the isolates in Pikovskaya's media. Biofertilizer was produced by inoculating each starter into the peat and wood charcoal as carrier material and was fermented for 4 days. Biofertilizer quality was determined by calculating the PSB cells number during 0, 30, 60, and 90 days storage time and the degree of biofertilizer acidity. The cell numbers of starter I, II, and III ranged from 8.2.1010-2.9.1011 CFU/g, 5.3.1010-2.9.1011 CFU/g, and 8.0.1010-2.9.1011 CFU/g, respectively in a period of storage 0-90 days. The highest cell number at the end of storage was found from biofertilizer produced by using peat, as carrier material which was kept at 40C (1,3.1011 CFU/g, starter I), (8.6.1010 CFU/g, starter II), and (9.6.1010 CFU/g, starter III). Biofertilizer acidity was relatively neutral, 6.38. Based on the results obtained, peat was revealed as a good carrier material and the quality of biofertilizers which were produced was still good

Potensi Limbah Sagu (Metroxylon SP.) Di Kecamatan Tebing Tinggi Barat Kabupaten Kepulauan Meranti Sebagai Substrat Penghasil Biogas

Biogas is one of alternative energy sources that fulfills future fuel needs. This studyaimed to analyse the potency of sago waste as fermentation substrate for biogasproduction. This research was done experimentally by employing sago waste materialsobtained from Sago Factory Nambus River from District West Tebing Tinggi Sub-province Meranti and fresh cow rumen liquid was obtained from slaughterhouse inPekanbaru. The biogas volume was calculated by measuring the volume of water thatwas pushed every 3 days, where the volume of water driven was proportional to thevolume of biogas produced. The bacterial cell numbers was counted using plate countmethod employing Nutrient Agar (NA). The substrate fermentation temperature and pHwere measured every 3 days. The highest biogas volume was produced by fermenter III(45760 ml) containing solid sago waste, liquid sago waste, and rumen liquid with ratio 1: 1 : 1 and the lowest was produced by fermenter II (1600 ml). The total bacterialinvolved in biogas production ranges from 1,87x10 5 – 2,87x10 8 CFU/ml samples. Thetemperature and pH of substrate fermetation was relatively constant during biogasproduction. The results showed that sago waste is potential as substrate for biogasproduction

Seleksi Kemampuan Bakteri Pelarut Fosfat Asal Bukit Batu-Riau dalam Menghasilkan Asam Sianida

Phosphate solubilizing bacteria (PSB) is beneficial bacteria which are not only capablein solubilizing organic phosphate into inorganic phosphate, but also producing cyanideacid (HCN). HCN plays a role in controling weed growth. The objective of this researchwas to screen the potency of indigenous PSB isolated from peat soil in producting HCN.The collections of PSB were subcultured in King'B broth. The ability of 152 isolates onHCN production were tested qualitatively using filter paper soaked with picric acid andNa 2 CO 3 . The result indicated that as many as 26 isolates (17.1%) were able to releasevarious levels of HCN production based on the appearing of brown, dark brown, orreddish brown colour on the filter paper

Enumerasi Total Populasi Mikroba Tanah Gambut Di Teluk Meranti Kabupaten Riau

Teluk Meranti is one of the peatland area in Riau province. Most of these lands have beenchanged into palm oil plantation, timber plantation, agricultural area and settlement. Theaim of this research was to analyze the impact of land use changes on soil physical-chemical characteristics and microbial cell number. Soil samples were taken from eightdifferent locations, namely primary forest as control, secondary forest, rubber plantation(15 monthsyears old), rubber forest (40-60 years old), palm oil plantation (7-8 years old),acacia plantation (2-3 years old), corn field, and cassava field. Microbial cell number wasdetermined by spread plate method, employing appropriate media for the growth ofbacteria, fungi and actinomycetes. The results showed that the soil humidity, soiltemperature, percentage of soil dry weight, water content, soil bulk density and pH rangedfrom 29,63-55,88%, 27-31,5 o C, 14,9-35,5%, 64,9-85,1%, 0,16-0,39 g/cm 3 and 3,63-4,00,respectively. The copiotrophic bacterial cell number ranged from 0,6x10 5 -1,8x10 5 CFU/gsoil where the highest population was at the palm oil plantation,whereas the oligotrophicbacterial cell number ranged from 0,5x10 5 -1,4x10 5 CFU/g soil where the highest populationwas at the palm oil plantation. The population of fungi ranged from 0,4x10 5 -1,0x10 5 CFU/gsoil where the highest population was at the corn field. The population of actinomycetesranged from 0,4x10 5 -10,7x10 5 CFU/g soil where the highest population was at the palm oilplantation. Land use changes caused microbial cell number increased. The results indicatedthat land use changes influenced the microbial cell numbers

Formulasi Biofertilizer Cair Menggunakan Bakteri Pelarut Fosfat Indigenus Asal Tanah Gambut Riau

Inefficient of P-uptake by plants during the application of chemical phosphorus (P) fertilizer results P-leaching to the aquatic environment. Therefore, the use of phosphate solubilizing bacteria (PSB) to enhance solubilization of P is necessary. The aims of this study were to find the best formulation in producing liquid biofertilizer which contained PSB and to analyze the storage time of liquid biofertilizer which were produced. As many as 4 selected PSB isolates (BB_UB6, BB_K9, BB_K2 and BB_HS13) were used to produce 3 combination starters. Liquid biofertilizers were produced by fermentation using three types of formulation, namely Pikovskaya\u27s medium, coconut water enriched with 2% molasses, and tofu waste water. The quality of liquid biofertilizer was determined by calculating the PSB cells number during 0, 30, and 60 days of storage time and by measuring the liquid biofertilizer acidity. Liquid biofertilizers produced were kept at room and refrigerator temperature. The results showed that the PSB cell numbers of starter I, II and III were higher in liquid biofertilizer that was formulated with coconut water which containing 2% of molasses until 60 days of storage time. The cell numbers of PSB ranged from 7,0×1010 - 2,82×1011 CFU/ml. In general, the PSB cell number was relatively stable when liquid biofertilizer was kept at room temperature. Based on this results, it can be concluded that the best formulation to produce liquid biofertilizer was the coconut water enriched with 2% molasses

Aktivitas Ligninolitik Beberapa Jamur Aphyllophorales dan Kemampuannya Mendegradasi Lignin pada Lindi Hitam

Fourteen local isolate Aphyllophorales fungi were screened their ligninolytic activity. The isolate with highest ligninolytic activity was tested it capability to degrade kraft blackliquor lignin. The biodegradability of black liquor is low because the presence of lignin and lignin derivative in the wastewater. These fungal were screened for ligninolytic activity by decolorization on solid mediacontaining RBBR dye. The ability of the fungal strains to biodegrade kraft black liquor lignin was performed by submerged fermentation condition with agitation and incubation time as treatment. The solid culture result in 3 isolates had ligninolytic activity and Ganoderma sp.BTA1 gave the highest ligninolytic. Agitation and incubation time influenced ligninbiodegradation of blackliquor significantly. Optimum condition for lignin biodegradation was at 200 rpm during 25 days with lignin reduction was 45,786%