Antibiofilm Effect of Different Irrigation Solutions Activated with KTP Laser

Objectives: The aim of this study was to evaluate the antimicrobial efficacy of Potassium Titanium Phosphate (KTP) laser-activated irrigation solutions on intraradicular Enterococcus faecalis biofilms in invitro conditions by using a scanning electron microscope. Materials and Methods: 120 single-root, single canal permanent mandibular premolar human teeth were used. Sterilization and disinfection of the teeth were performed. E. faecalis biofilms were obtained after 4 weeks by re-inoculation procedures. Each group was divided into 6 groups consisting of 20 roots and root canal disinfection protocols were applied using irrigation solutions with 5.25% NaOCl, saline, super-oxidized water solution, 8ppm ozonated water, 2% CHX, 17% EDTA, all activated by KTP laser. It was smear-planted to a solid medium which split as before and after the disinfection applications for the aim of Counting Microorganism colonies from root canals and data were evaluated statistically. In this statistical evaluation one way ANOVA and Tukey tests were used. Before and after the irrigation procedures for presence and elimination of biofilm the root canals were processed for scanning electron microscopy and biofilm was examined on the standard images. Results: As a result of the statistical comparison performed among all groups, while NaOCl ensures the highest amount of elimination as a positive control group, the lowest amount of bacterial elimination was detected in the SF group that applied as the negative control group (P0,05), EDTA showed the lowest antibiofilm effect (

The effect of autogenous bone graft mixed with recombinant human vascular endothelial growth factor on bone regeneration.

INTRODUCTION: Bone regeneration depends on vascularization in the pertaining site. This study aims to investigate autogenous bone grafts mixed with recombinant human vascular endothelial growth factor (rhVEGF) effect on bone regeneration in rat mandibular bone defect. MATERIAL METHODS: Using 32 Wistar Albino rats, our experimental study consists of 4 groups: Group1 (control group), the defect was empty; Group 2, autogenous bone graft only; Group 3, gelatin sponge plus rhVEGF applications; Group 4, autogenous bone graft plus rhVEGF applications. The rats were sacrificed on the 28th day after the operation. New bone regeneration was analyzed histologically and immunohistochemically. RESULTS: Our histological analyses revealed that new bone regeneration in Group 3 was enhanced in comparison to Group 1 and Group 2. However, autogenous bone grafts combined with rhVEGF provided the best outcome in conjunction with the increased remodeling of the new bone. CONCLUSIONS: In the light of our results, it can be concluded that autogenous bone grafts in combination with rhVEGF can, potentially, enhance neovascularization and bone regeneration