Effects of inoculum density of Fusarium oxysporum f. sp. ciceri race 1 and 2 on chickpea wilt

Studies on inoculum densities of the chickpea wilt pathogen F. oxysporum f.sp. ciceris race 1 and 2 with 3 chickpea cultivars representing early wilter (highly susceptible), late wilter (moderately susceptible) and resistant were conducted in a glasshouse at 25°C. Highly susceptible cv. JG 62 showed 100% mortality at 15 days for race 1 and 17 days for race 2 at higher inoculum density. At the low inoculum density, the wilting was delayed and at this inoculum level 2 other cultivars showed no mortality. The wilt resistant cultivar cv. WR 315 and moderately susceptible cultivar cv. K 850 remained completely free from wilt symptoms for up to 60 days while cv. JG 62 wilted in 27 days with F. o. f.sp. ciceris populations ranging from 4233 to 4667 propagules/g soil. Xylem browning in the root and collar region was recorded in cv. K 850. The F. o. f.sp. ciceris population of race 1 and 2 increased with age of the plant in soils with wilt susceptible, moderately susceptible and resistant chickpea cultivars. Moderately susceptible and resistant cultivars supported the multiplication of F. o. f.sp. ciceris propagules in the soil though the plants were free of wilt symptoms for up to 60 days after planting. The multiplication ratio was higher at low inoculum density of the propagules compared with the higher inoculum density for both the races of F.o. f.sp. ciceris. As the inoculum density increased the multiplication ratio decreased

Synthesis of substituted benzo[e][1,3]oxazino analogs

325-327A synthesis of substituted benzo[e][1,3]oxazin-4-one analogs has been carried out by two methods. One of the common procedures involves refluxing in situ generated imine with salicylic acid while other method involves one-pot three component condensation reaction between aldehyde, amine and salicylic acid using DCC. The synthesized compounds have been characterized by IR, 1H and 13C NMR spectroscopy. Melting points reported are uncorrected

Multilocational testing of pigeonpea for broad-based resistance to sterility mosaic in India

During 1978-83, 88 pigeon pea lines resistant to sterility mosaic (SM) from different research centres in India were tested at 10 locations (Badnapur, Bangalore, Dholi, Pantnagar, Faizabad, Kanpur, Ludhiana, Patancheru, Vamban and Varanasi) to identify lines with stable and broad-based resistance. The multilocation evaluation was carried out through the joint Indian Council of Agricultural Research and the ICRISAT Uniform Trial for Pigeon Pea Sterility Mosaic Resistance. SM resistant genotypes were identified at each of the 10 locations. Lines ICP 7867, ICP 10976 and ICP 10977 were resistant or tolerant at all 10 locations. These lines are now being used by breeders at ICRISAT as well as in the Indian national programme for developing SM resistant and high yielding cultivars

Blind docking of 4-Amino-7-Chloroquinoline analogs as potential dengue virus protease inhibitor using CB Dock a web server

Currently, there is no approved drug to combat dengue. Various quinoline derivatives are known for potential antimalarial, antiviral activities, etc. In the present work docking between 4-Amino-7-Chloroquinoline analogs was performed with dengue virus NS2B/NS3 protease using CB dock, a web server. Lys74, Ile165, Val147, Asn152, Asn167, Trp83 and Leu149 amino acid residues were found to be in contact with designed 4-Amino-7-Chloroquinoline analogs. Different modes of binding like hydrogen bonding, hydrophobic interactions, etc with designed compounds improve potential anti-dengue characteristics in silico. ADME results are in acceptable range

Synthesis and Spectroscopic Investigation of Binding of Novel Thiazolo [2, 3-a] isoquinoline analog with Bovine Serum Albumin

A condensation reaction between 1-cyclobutyl-3,4-dihydroisoquinoline and thioglycolic acid was carried out using DCC yields 10b-cyclobutyl-5,6-dihydro-2H-thiazolo [2, 3-a] isoquinolin-3(10bH)-one [CBIQTGA]. The interaction of CBIQTGA with Bovine Serum Albumin (BSA) was studied using UV spectroscopy, spectrofluorimetry and circular dichroism (CD) techniques

Synthesis and spectroscopic investigation of binding of novel Thiazolo [2, 3-a] isoquinoline analog with bovine serum albumin

1081-1085A condensation reaction between 1-cyclobutyl-3,4-dihydroisoquinoline and thioglycolic acid has been carried out using DCC yields 10b-cyclobutyl-5,6-dihydro-2H-thiazolo [2, 3-a] isoquinolin-3(10bH)-one [CBIQTGA]. The synthesized compound is characterised by using spectroscopic techniques. The interaction of CBIQTGA with bovine serum albumin has been studied using UV spectroscopy, spectrofluorimetry and circular dichroism techniques

Multilocational Evaluation of Pigeonpea for Broad-Based Resistance to Fusarium Wilt in India

Nine-hundred and fifty-nine plgeonpea germplasm and breeding lines were evaluated for resistance to wilt caused by Fusarium udum Butler at 12 locations in India over a period of 7 years between 1984 and 1990. ICP 8863,9174,12745, ICPL333,8363,88047, BWR 370, DPPA 85-2,85-3,85-8,85–13,85–14 and Bandapalera were resistant or moderately resistant at 7 to 10 out of 12 locations for 3 to 5 years with an average wilt incidence of less than 15%

Multi-location evaluation of pigeonpea (Cajanus cajan) for broad-based resistance to sterility-mosaic disease in India

A total of 141 germplasm accessions and 725 breeding lines of pigeon pea were evaluated for resistance to pigeon pea sterility mosaic virus at 13 field locations in India from 1983-84 to 1989-90. Some 50 seeds per test entry were sown at each location and the trials were artificially inoculated by either leaf-stapling (in which 10-15-day-old seedlings were stapled with diseased leaves harbouring mites) or the infector-hedge method (with the susceptible variety NP(WR)15 sown on the border of the nursery 4-6 months in advance of the normal sowing date). The breeding lines included in the evaluation were newly bred, high-yielding lines entered into the Pigeon Pea Co-ordinated trials by ICRISAT and the All-India Co-ordinated Pulses Improvement Project. ICP7182 was used as the susceptible control line. Information is presented on lines and accessions showing broad-based resistance (percentage infection at maturity) during the trial period at each location. Line ICP7035 from Madhyha Pradesh, which contains DSLR55 in its pedigree, was resistant at 12 locations and some 18 lines were resistant at 10 locations

Synthesis of substituted benzo[e][1,3]oxazino analogs

A synthesis of substituted benzo[e][1,3]oxazin-4-one analogs has been carried out by two methods. One of the common procedures involves refluxing in situ generated imine with salicylic acid while other method involves one-pot three component condensation reaction between aldehyde, amine and salicylic acid using DCC. The synthesized compounds have been characterized by IR, 1H and 13C NMR spectroscopy. Melting points reported are uncorrected

Blind docking of 4-Amino-7-Chloroquinoline analogs as potential dengue virus protease inhibitor using CB Dock a web server

55-57Currently, there is no approved drug to combat dengue. Various quinoline derivatives are known for potential antimalarial, antiviral activities, etc. In the present work docking between 4-Amino-7-Chloroquinoline analogs was performed with dengue virus NS2B/NS3 protease using CB dock, a web server. Lys74, Ile165, Val147, Asn152, Asn167, Trp83 and Leu149 amino acid residues were found to be in contact with designed 4-Amino-7-Chloroquinoline analogs. Different modes of binding like hydrogen bonding, hydrophobic interactions, etc with designed compounds improve potential anti-dengue characteristics in silico. ADME results are in acceptable range