Clinico-morphological manifestations of atherosclerotic lesions of cerebral basilar arteries

Background: Endothelial dysfunction is an early sign of atherosclerosis, which favors the increase of vascular permeability, activation of mast cells and migration of leukocytes, lymphocytes, macrophages, platelet adhesion, smooth muscle cell proliferation and eventual vasospasm, which together determine a proinflammatory status. Angiogenesis is an important pathogenic element of atherosclerosis in stages of complicated plaques, along with mast cells and macrophages. Histotopographical analysis of the distribution of newly-formed vessels as a feature of angiogenesis, expression of mast cell and macrophage in different types of plaques in different arterial vessels in patients with atherosclerosis and metabolic syndrome complicated by atherosclerosis. Material and methods: We studied 34 patients with cerebral artery atherosclerosis. To determine the expression of mast cells in the affected vessels, we used anti-MCT. Macrophages were identified using specific marker CD-68 and newly-formed vessels respectively through the application of CD-105 (endoglin). Results: Assessment of the results was based on the final determination of the density and intensity of reaction, as reflected in the quantitative ratio of the different areas of atheromatous plaques. Stained positive mast cells, macrophages and newly-formed vessels have been found in several types of atherosclerotic plaques, especially in adventitia and in the immediate vicinity of the plaques and subendothelial layers. We found a statistical correlation between the type of plaque and clinical data. Conclusions: Immunohistochemical method is efficient for the determination of mast cells, macrophages and newly-formed vessels of atherosclerotic plaques, directly reflecting many important pathogenic factors of atherogenesis in patients with atherosclerosis of the brain arteries

Disorders circulation in osteochodrosis of the lumbar intervertebral disc

Atherosclerosis is one of the etiological factors to initiate the degeneration disc studied in detail by histological investigations stained with haematoxylin-eosin, silver impregnation and immunohistochemical examination with antibodies CD34, CD68 and MCT (mastocyte cell tryptse) in immunohistochemicalLoboratorul Timisoara, leaded by Marius Raica. All are made on a sample of 20 cases where prevailed the L1-L5 vertebral arteries in people with different pathologies somatic cardiac death, age and sex of MCH “Sfânta Treime”. Atherosclerosis with vascular wall thickening has a structural impact on the viability of intervertebral disc, initially being affected nucleus pulposus, annulus and then through a series of mechanisms for splitting fragmented collagen fi bers, and fi nally proliferation with sclerosis. The vertebral arteries affected are 5, lacking intimate vascular event (5%), 3 cases, faint, moderate in 10 cases – handed (50%), and 6 cases – preserved.Атеросклероз является одним из этиологических факторов для начала дегенеративных изменений в позвоночных дисках. Подробно изучены гистологические исследования с окрашиванием гематоксилин-эозином, oрсеином, пропитка серебром, а также иммуногистохимические исследования с антителами CD34, CD68 и MCT (mastocyte cell tryptse) в Лаборатории г. Тимишоара, руководитель – Marius Raica. Все они исследованы в 20 случаях на поясничных позвоночных артериях (1-5 позвонки) у людей умерших от различных соматических заболеваниях, разные по возрасту и полу, в МКБ «Sfânta Treime». Атеросклероз с утолщением стенок влияет на структурную жизнеспособность межпозвоночного диска, первоначально на студенистого ядро, потом на фиброзное кольцо, а затем, через ряд механизмов, ведёт к фрагментированию коллагеновых волокон, и, наконец, фибробласты распространяются со склерозом. В позвоночных артериях пораженных атеросклерозом, в 5-ти случаях отсутствует интима в стенках сосудов (5%), в 3-х случаях – слабо-умеренное, в 10-ти случаях – умеренное (50%), а также в 6 случаях интима сохранилась

Teeth as a sourse of stem cells

Catedra Stomatologie Teraeutică FECMF, USMF „Nicolae Testimiţanu” Laboratorul de enginerie tisulară și cultivarea celulelorTooth loss compromises human oral health. Although several prosthetic methods, such as artificial denture and dental implants, are clinical therapies to tooth loss problems, they are thought to have safety and usage time issues. Recently, tooth tissue engineering has attracted more and more attention. Stem cell based tissue engineering is thought to be a promising way to replace the missing tooth. Mesenchymal stem cells are multipotent stem cells which can differentiate into a variety of cell types. This review outlines the recent progress in mesenchymal stem cell research and use in tooth regeneration [1, 2]. Pierderea dinților compromite sănătatea umană orală. Deşi, mai multe metode de tratament cum ar fi protezele artificiale şi implanturile dentare si-au demonstrat siguranţă de utilizare in timp. Recent, ingineria tisulară a capătat o vastă amploare.Celulele stem sunt considerate a fi o modalitate promiţătoare pentru a înlocui dintele lipsă. Celulele stem mezenchimale sunt celule stem pluripotente care se pot diferenția într-o varietate de tipuri de celule. Aceast articol prezintă progresele recente în cercetarea celulelor stem mezenchimale şi utilizarea lor

Inflammation and immunohistochemical profile of the lymphocyte population in the prostate adenoma and the limit of residual prosthetic surgical capsule after adenomectomy

Catedra de morfopatologie USMF ”Nicolae Testimițanu”, Catedra de urologie și nefrologie chirurgicală USMF ”Nicolae Testimițanu”, Catedra de histologie, citologie și embriologie, Universitatea din Oradea, România, Facultatea de Medicină, IMSP Spitalul Clinic Municipal „Sf.Treime”, Secția de urologie, Al VII-lea Congres de Urologie, Dializă și Transplant Renal din Republica Moldova cu participare internațională 19-21 iunie 2019Studiul constă în: evaluarea inflamației în adenomul prostatic, rolului inflamației și profilului limfocitar ca factor predictiv în evoluția adenomului prostatic; evaluarea particularităților capsulei chirurgicale reziduale și a importanței acesteia în aplicarea hemostazei locale după adenomectomie. Studiul actual este de tip prospectiv și include un eșantion din 118 cazuri (98 cu adenom de prostată rezolvat chirurgical la vârsta medie de 68,6 ±1,05 ani și 20 de prostate de la decedați incidental la vârsta cuprinsă între 21-67 de ani în lipsa intravitală a statutului morbid urogenital stabilit. S-a stabilit prezența corelațiilor prostatitei cronice și a adenomului prostatic, pe un fundal patologic preexistent la nivelul capsulei chirurgicale fiind o prostatită marginală pozitivă cronică activă reziduală după adenomectomie. Frecvența majoră a limfocitelor-T poate rezulta din răspunsul imunologic la diverse leziuni, fiind și un marker al rolului autoimun în evoluția adenomului. Prostatita marginală pozitivă este un impact semnificativ în hemostaza locală și în evoluția perioadei intra- și post-operatorii, servind ca factor predictiv pentru necesitatea monitorizării lojei adenomului și utilizarea unui tratament complementar adecvat.The study consists of evaluation of inflammation in the prostatic adenoma, the role of inflammation and lymphocytic infiltrate as a predictive factor in the evolution of the prostatic adenoma, evaluation of the particularities of the residual surgical capsule and its importance in the application of local hemostasis after adenomectomy. The current study is a prospective study of a sample of 118 cases (98 with prostate adenoma resolved surgically at medium age of 68.6 ± 1.05 years and 20 prostates from incidental deaths at the age of 21-67 years in the absence intravital status of the established urogenital morbidity status. It has been established the presence of correlations of chronic prostatitis and prostate adenoma and the real pathological background preexisting in the surgical capsule, being a chronic active positive marginal prostatitis residual after adenomectomy. Major T-lymphocytes frequency can result from the response immunologically to various lesions as well as a marker of the autoimmune role in evolution of adenoma. Positive marginal prostatitis is a significant impact on local haemostasis and the evolution of the intra- and postoperative period, serving as a predictive factor for necessity of monitoring the adenoma lodge and using appropriate complementary therapy