190 research outputs found
Depletion interactions modulate the binding between disordered proteins in crowded environments
Highly sensitive coulometric titration of oxygen for the characterization of solid materials at elevated temperatures
A new setup for characterization of solid material oxygen exchange and
conductivity in a broad oxygen partial pressure range and at elevated
temperatures is presented. The development target of this setup is directed
towards the detection of ultra-low amounts of exchanged oxygen. For this,
electrochemical cells made of yttria-stabilized zirconia (YSZ) were optimized
and applied in a flow-through arrangement. The design and process measures
enable a lower limit of detection below 100 pmol of exchanged oxygen.
Furthermore, the system characteristics concerning oxygen dispersion,
titration efficiency and electrode kinetics are described.</p
Morphology and elasticity of waterborne acrylic pressure-sensitive adhesives investigated with atomic force microscopy
Russian language as a key factor in aiding the integration of foreign students in higher education
Dietary supplements and herbal medicine toxicities—when to anticipate them and how to manage them
The rheological behaviour of suspensions of fat particles in oil interpreted in terms of a transient-network model
Depletion interactions modulate the binding between disordered proteins in crowded environments
Intrinsically disordered proteins (IDPs) abound in cellular regulation. Their interactions are often transitory and highly sensitive to salt concentration and posttranslational modifications. However, little is known about the effect of macromolecular crowding on the interactions of IDPs with their cellular targets. Here, we investigate the influence of crowding on the interaction between two IDPs that fold upon binding, with polyethylene glycol as a crowding agent. Single-molecule spectroscopy allows us to quantify the effects of crowding on a comprehensive set of observables simultaneously: the equilibrium stability of the complex, the association and dissociation kinetics, and the microviscosity, which governs translational diffusion. We show that a quantitative and coherent explanation of all observables is possible within the framework of depletion interactions if the polymeric nature of IDPs and crowders is incorporated based on recent theoretical developments. The resulting integrated framework can also rationalize important functional consequences, for example, that the interaction between the two IDPs is less enhanced by crowding than expected for folded proteins of the same size
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