1 research outputs found
Revealing the Effect of Photothermal Therapy on Human Breast Cancer Cells: A Combined Study from Mechanical Properties to Membrane HSP70
Hyperthermia-induced
overexpression of heat shock protein 70 (HSP70)
leads to the thermoresistance of cancer cells and reduces the efficiency
of photothermal therapy (PTT). In contrast, cancer cell-specific membrane-associated
HSP70 has been proven to activate antitumor immune responses. The
dual effect of HSP70 on cancer cells inspires us that in-depth research
of membrane HSP70 (mHSP70) during PTT treatment is essential. In this
work, a PTT treatment platform for human breast cancer cells (MCF-7
cells) based on a mPEG-NH2-modified polydopamine (PDA)-coated
gold nanorod core–shell structure (GNR@PDA-PEG) is developed.
Using the force-distance curve-based atomic force microscopy (FD-based
AFM), we gain insight into the PTT-induced changes in the morphology,
mechanical properties, and mHSP70 expression and distribution of individual
MCF-7 cells with high-resolution at the single-cell level. PTT treatment
causes pseudopod contraction of MCF-7 cells and generates a high level
of intracellular reactive oxygen species, which severely disrupt the
cytoskeleton, leading to a decrease in cellular mechanical properties.
The adhesion maps, which are recorded by aptamer A8 functional probes
using FD-based AFM, reveal that PTT treatment causes a significant
upregulation of mHSP70 expression and it starts to exhibit a partial
aggregation distribution on the MCF-7 cell surface. This work not
only exemplifies that AFM can be a powerful tool for detecting changes
in cancer cells during PTT treatment but also provides a better view
for targeting mHSP70 for cancer therapy