Conceptual designs of dipole magnet for muon collider ring

Conceptual designs of a superconducting dipole magnet for a Storage Ring of a Muon Collider with a 1.5 TeV center of mass (c.o.m.) energy and an average luminosity of 10 34 cm-2s-1 are presented. In contrast to proton machines, the dipoles for the Muon Collider should be able to handle ~0.5 kW/m of dynamic heat load from the muon beam decays. The magnets are based on Nb3Sn superconductor and designed to provide an operating field of 10 T in the 20-mm aperture with the critical current margin required for reliable machine operation. The magnet cross-sections were optimized to achieve the best possible field quality in the aperture occupied by beams. The developed mechanical structures provide adequate coil prestress and support at the maximum level of Lorentz forces in the coil. Magnet parameters are reported and compared with the requirements.Comment: 4 pp. Applied Superconductivity Conference (ASC 2010), 1-6 Aug 2010: Washington, D.

Making complex things simpler: modern tools to edit the plant genome

There are several technologies for plant genome editing, of which the most simple and universal is CRISPR/Cas. Currently, this technology is widely used for gene knockout, deleting genome fragments and inserting exogenous sequences in the plant genome. For each of these applications, many different types of genetic tools have been developed that are used by various research groups to solve specific problems. The CRISPR/Cas technology for plant genome editing is at an early stage of optimization, which is reflected by the ongoing search for the most effective, simple and flexible techniques. As a result, experimental work has to be preceded by a rather long and laborious process of selecting a genetic tool that will be optimal for a specific experimental task. In our review we describe the main variants of the CRISPR/Cas technology used to edit a plant genome. We classify them in terms of experimental tasks solved, major components and technology performance. In the first half of the review a detailed description of two major components of CRISPR/Cas technology – nuclease and guide RNA – is given, the effect of structural features of these elements on editing efficiency is analyzed. Experimental data on the relationship between editing efficiency and nucleotide sequence of guide RNA are generalized. We also give the characteristic for different variants of nucleases used for plant genome editing and discuss their benefits for different experimental purposes. In the second half of the review various strategies for expression of CRISPR/Cas elements in plant cells, in particular, advantages and disadvantages of stable transformation and transient expression, are discussed. The effect of various regulatory elements of genes encoding nuclease and guide RNA on editing efficiency is described. Special emphasis is placed on the techniques of increasing targeted gene replacement efficiency

Characteristics of Somatic Status and Individual Biochemical Markers in Patients with Retinal Vein Occlusion

Retinal vein occlusion is one of the most severe vascular lesions of the organ of vision, usually accompanied by somatic pathology.The aim of the study was to study the somatic status and individual biochemical markers in patients with retinal vein occlusion and comparison in groups opposing the effectiveness of anti-VEGF therapy.Methods. 84 patients with macular edema were examined on the background of retinal vein occlusion, who underwent intravitreal injections of an angiogenesis inhibitor for therapeutic purposes. Further, depending on the effectiveness of treatment, the patients were divided into 2 groups, in which a retrospective comparative analysis of somatic status and laboratory data was performed.Results. It was shown that patients with occlusion of the retinal veins have significant impairments in terms of somatic status, blood biochemical parameters and coagulogram. Moreover, there is a direct relationship between the severity of somatic changes and ocular manifestations of the disease. The data obtained reflect a clear tendency in patients with occlusion of the retinal veins to a high risk of cardiovascular complications, including occlusive lesions. In patients with an insufficient treatment effect, a more severe course of arterial hypertension with an increased risk of stratification was detected.Conclusion. The somatic status and laboratory parameters of patients with retinal vein occlusion are significantly different from the norm, which must be taken into account in the prognosis of the course of an ophthalmologic disease

Optics implications of implementing Nb3Sn magnets in the LHC phase 1 upgrade

CERN has encouraged the US-LARP collaboration to participate in Phase I of the LHC luminosity upgrade by analyzing the benefits gained by using Nb3Sn technology to replace the functionality of select NbTi magnets that CERN is committed to construct. Early studies have shown that the much higher gradients (shorter magnetic lengths) and temperature margins (quench stability) of Nb3Sn magnets compared to their NbTi counterparts is favorable--allowing the insertion of additional absorbers between Q1 and Q2, for example. This paper discusses the relative merits of the NbTi and Nb3Sn options

Development of the Multi-Analyte Test for Immune-Chromatographic Detection of Botulinum Toxins

Designed is the multi-analyte test for simultaneous immune chromatographic detection of A & B type botulinum neurotoxins (BT), using colloid gold nanoparticles. It is meant for food and environmental samples' analysis. The sensitivity of simultaneous BT detection of the A (30 ng/ml) and B (10 ng/ml) types is as high as that of mono-analytical tests, designed for one type BT detection. The test is demonstrated to be a specific one and can be used for BT detection in food stuffs

Construction of Immune-Chromatographic Indicator Elements for Burnet Rickettsia Detection

mc/ml, and the elapsed time – 20 minutes. The IE is specific to other members of the family Ricketsiaceae, for instance to R. prowazekii , to antigen complexes of R. prowazekii and R. sibirika , as well as to vaccinia virus (L-IVP strain). The IE engineered can be used for rapid indication of Burnet Rickettsia at different stages of laboratory investigation. Span time reduction, lack of necessity to perform any accessory technological operations, visual and (or) automatic registration of the results build up premises to observe immune-chromatographic method for Burnet Rickettsia detection as one of the alternatives for identification of these microorganisms under field conditions when monitoring ambient environment objects