MECHANOCHEMICAL ACTIVATION OF PHARMACEUTICAL SUBSTANCES AS A FACTOR FOR MODIFICATION OF THEIR PHYSICAL, CHEMICAL AND BIOLOGICAL PROPERTIES

Objective: Study the influence of the mechanical preparation methods (grinding, fluidization) of solid pharmaceutical substances (PS) and herbal raw material on their physicochemical properties and biological activities. Methods: Test substances and solvents-Lactose monohydrate (DFE Pharma, Germany). Sodium chloride, bendazol hydrochloride (all Sigma-Aldrich, USA) and herbal raw material (Callisia fragrans). The dispersity and native structure of pharmaceutical substances were analyzed by several methods: optical microscopy–Altami BIO 2 microscope (Russia); low angle laser light scattering (LALLS) method (Malvern Instruments, UK); Spirotox method–Quasichemical kinetic of cell transition of cellular biosensor Spirostomum ambiguum; Fourier-transform infrared spectroscopy–the analysis in the middle IR region was carried out using an IR Cary 630 Fourier spectrometer (Agilent Technologies, USA). The analysis of dried leaves of C. fragrans before and after mechanical activation was performed using Shimadzu EDX-7000 X-ray fluorescence spectrophotometer without mineralization (Shimadzu, Japan). Results: It was established that the mechanical change, such as dispersion and drying, alters the biological activity of PS and herbal raw materials. The observed increase in the influence of the dispersed substance on the biosensor S. ambiguum is quantitatively estimated from the values of the activation energy (obsEa), which turns to be valued 1,5 (P≤0,05) times more than for the native form substance. In the study of the dependence of the availability of chemical elements K, Ca, Zn on the degree of dispersion of herbal raw materials was established a quantitative 4-fold (P≤0,05) increase in the concentration of elements in mechano-activated raw materials. Conclusion: By the example of the biological model of Spirotox (single-celled biosensor S. ambiguum) and herbal raw materials obtained from C. fragrans, the increase of biological activity of PS at the dispersion of initial preparations was proved

Effect of deuterium on the morpho-functional characteristics of normal and cancer cells in vitro

Objective: The aim of our study was to describe effects of different deuterium concentration on morphology and migratory activity of normal stem cells and cancer cell lines in vitro. Materials and methosj51 Water with different deuterium content was used for the culture media preparation: deuterium-depleted water (ddw, D/H = 1 ppm), deutereted (deuterium-rich) water (D/H = 99 abs. At. D%); water with natural deuterium content (MiliQ system) (D/H = 150 ppm) served as control. The cells were cultured in DMEM: F12 supplemented with 10% FBS, 2 mM L-glutamine, and 1 ng/mL FGF-2 in a multi-gas incubator at 5% CO2 and 5% O-2. The morphology of adipose-derived mesenchymal stem cells (ADSCs) was observed after 24 and 72 hours cultivation in experimental media. After incubating for 0, 12, 24, and 48 hours, the gap width of scratch re-population was measured and recorded, and then compared with the initial gap size at 0 hours. Results: High deuterium concentration in culture medium leads to significant morphological changes in normal ADSCs that are associated with cellular stress. Moreover, the migratory activity of ADSCs was inhibited under the deutereted water. At the same time. ddw did not influence morphology or migration of ADSCs. Bothdeutereted water and ddw strongly inhibited migration of cancer cell lines A549 and HT29. Conclusion: Our findings demonstrated that deuterium could act as regulator of biological properties of normal and cancer cells in vitro. However, the mechanisms that underlie the deuterium-mediated effect on different cellular types need to be further investigated

Correlation biological activity and physicochemical properties of humic acids in aqueous solutions

Objective. The biological activity of humic acids (HA), considering them as open non-equilibrium systems, in which properties are determined by the state or level of organization of the system. Methods. The dependence of physicochemical properties of HA on their concentration (optical and sorption properties, ζ-potential and electrical conductivity, average size of optical homogeneities) were analyzed by several methods: optical microscopy; low-angle laser light scattering (LALLS) method on a Zetasizer Nano ZS analyzer; absorption on a Shimadzu UV-3600 spectrophotometer; chemical analysis on an ICP-QMS Agilent 7500CE spectrometer. Biological activity of HA was studied on the crustacean Daphnia magna and the monocotyledonous plants Allium cepa L. Results. It was established that the nature of the HA effect on biological objects is non-linear and has an extremum at 1 mg•L-1. It was shown that the mineralization of solutions affects the biological activity of HA. So in the presence of the addition of mineral salts, for D. magna the effect of HA is insensible, while for A. cepa L. it changes its direction. In the absence of mineral salts at HA concentrations < 5-10 mg•L-1, a sharp drop in the survival of D. magna and suppression of root growth of A. cepa L. was observed, and in saline solution – stimulation of root growth. It has been established that the concentration range of 5-10 mg•L-1 was also critical for concentration changes in a number of physicochemical parameters of aqueous solutions of HA, which makes it possible to consider it a phase transition characterized by new properties. Findings. It was shown that changes in various properties of aqueous solutions of HA, including biological activity, were characterized by non-linear concentration dependencies with the presence of intermittent changes indicating qualitative rearrangements in the system. © 2020, HARD Publishing Company. All rights reserved

Formation of a test system and the choice of test criteria when biotesting natural waters

Described are main problems of biotesting natural water. Defined are principles for formation of a test system and factors influencing it. Proposed are main areas for the choice of sensitive test reaction of organisms and test criteria for assessing the quality of natural water by the results of their biotesting. © 2016, Allerton Press, Inc

Deuterium effect on proliferation and clonogenic potential of human dermal fibroblasts in vitro

[No abstract available

Formation of a test system and the choice of test criteria when biotesting natural waters

Described are main problems of biotesting natural water. Defined are principles for formation of a test system and factors influencing it. Proposed are main areas for the choice of sensitive test reaction of organisms and test criteria for assessing the quality of natural water by the results of their biotesting. © 2016, Allerton Press, Inc

Effect of deuterium on the morpho-functional characteristics of normal and cancer cells in vitro

Objective: The aim of our study was to describe effects of different deuterium concentration on morphology and migratory activity of normal stem cells and cancer cell lines in vitro. Materials and methosj51 Water with different deuterium content was used for the culture media preparation: deuterium-depleted water (ddw, D/H = 1 ppm), deutereted (deuterium-rich) water (D/H = 99 abs. At. D%); water with natural deuterium content (MiliQ system) (D/H = 150 ppm) served as control. The cells were cultured in DMEM: F12 supplemented with 10% FBS, 2 mM L-glutamine, and 1 ng/mL FGF-2 in a multi-gas incubator at 5% CO2 and 5% O-2. The morphology of adipose-derived mesenchymal stem cells (ADSCs) was observed after 24 and 72 hours cultivation in experimental media. After incubating for 0, 12, 24, and 48 hours, the gap width of scratch re-population was measured and recorded, and then compared with the initial gap size at 0 hours. Results: High deuterium concentration in culture medium leads to significant morphological changes in normal ADSCs that are associated with cellular stress. Moreover, the migratory activity of ADSCs was inhibited under the deutereted water. At the same time. ddw did not influence morphology or migration of ADSCs. Bothdeutereted water and ddw strongly inhibited migration of cancer cell lines A549 and HT29. Conclusion: Our findings demonstrated that deuterium could act as regulator of biological properties of normal and cancer cells in vitro. However, the mechanisms that underlie the deuterium-mediated effect on different cellular types need to be further investigated

Deuterium effect on proliferation and clonogenic potential of human dermal fibroblasts in vitro

[No abstract available

ИЗМЕНЕНИЯ МЕТАБОЛИЧЕСКОЙ АКТИВНОСТИ НОРМАЛЬНЫХ И РАКОВЫХ КЛЕТКОК ЧЕЛОВЕКА IN VITRO ПРИ РАЗНОЙ КОНЦЕНТРАЦИИ ДЕЙТЕРИЯ В КУЛЬТУРАЛЬНОЙ СРЕДЕ

In current study we have shown the in vitro normal and cancer cells in deuterated growth medium demonstrated decrease in metabolic activity. In contrast, in deuterium-depleted medium there was an increase metabolic activity.В настоящем исследовании мы показали, что нормальные и раковые клетки in vitro в дейтерированной ростовой среде демонстрируют снижение метаболической активности, а в обедненной дейтерием среде повышение