Diverzita kryptosporidií u pěvců

This study was aimed on research of biology and diversity of Cryptosporidium in perching birds (Passeriformes). Presence of Cryptosporidium oocyst and specific DNA in faecal samples was detected by microscopy and molecular methods using nested PCR. Biological properties and intensity of infection of Cryptosporidium were examined and described in both experimentally and naturally infected animals

Kryptosporidie a kryptosporidióza chovaných a zdivočelých nutrií

Cryptosporidium spp., common parasite of vertebrates, remain poorly studied in wildlife. This study describes the novel Cryptosporidium species adapted to nutria (Myocastor coypus). A total of 247 faecal samples of feral and farmed nutrias were collected from locations in the Czech Republic and Slovakia and examined for Cryptosporidium spp. oocysts and specific DNA at the SSU, actin, HSP70, and gp60 loci

\kur{Cryptosporidium myocastoris} n. sp. (Apicomplexa: Cryptosporidiidae), druh adaptovaný na nutrie (\kur{Myocastor coypus})

Cryptosporidium spp., common parasite of vertebrates, remain poorly studied in wildlife. This study describes the novel Cryptosporidium species adapted to nutria (Myocastor coypus). A total of 150 faecal samples of feral nutrias were collected from locations in the Czech Republic and Slovakia and examined for Cryptosporidium spp. oocysts and specific DNA at the SSU, actin, HSP70, and gp60 loci

Cryptosporidium myocastoris n. sp. (Apicomplexa: Cryptosporidiidae), the Species Adapted to the Nutria (Myocastor coypus)

Cryptosporidium spp., common parasites of vertebrates, remain poorly studied in wildlife. This study describes the novel Cryptosporidium species adapted to nutrias (Myocastor coypus). A total of 150 faecal samples of feral nutria were collected from locations in the Czech Republic and Slovakia and examined for Cryptosporidium spp. oocysts and specific DNA at the SSU, actin, HSP70, and gp60 loci. Molecular analyses revealed the presence of C. parvum (n = 1), C. ubiquitum subtype family XIId (n = 5) and Cryptosporidium myocastoris n. sp. XXIIa (n = 2), and XXIIb (n = 3). Only nutrias positive for C. myocastoris shed microscopically detectable oocysts, which measured 4.8–5.2 × 4.7–5.0 µm, and oocysts were infectious for experimentally infected nutrias with a prepatent period of 5–6 days, although not for mice, gerbils, or chickens. The infection was localised in jejunum and ileum without observable macroscopic changes. The microvilli adjacent to attached stages responded by elongating. Clinical signs were not observed in naturally or experimentally infected nutrias. Phylogenetic analyses at SSU, actin, and HSP70 loci demonstrated that C. myocastoris n. sp. is distinct from other valid Cryptosporidium species