Evolutionary Analyses Reveal Diverged Patterns of SQUAMOSA Promoter Binding Protein-Like (SPL) Gene Family in Oryza Genus

The SPL (SQUAMOSA promoter binding protein-like) gene family is one of the plant-specific transcription factor families and controls a considerable number of biological functions, including floral development, phytohormone signaling, and toxin resistance. However, the evolutionary patterns and driving forces of SPL genes in the Oryza genus are still not well-characterized. In this study, we investigated a total of 105 SPL genes from six AA genome Oryza representative species (O. barthii, O. glumipatula, O. nivara, O. rufipogon, O. glaberrima, and O. sativa). Phylogenetic and motif analyses indicated that SPL proteins could be divided into two distinct lineages (I and II), and further studies showed lineage II consisted of three clades (IIA, IIB, and IIC). We found that clade I had comparable structural features with clade IIA, whereas genes in clade IIC displayed intrinsic differences, such as lower exon numbers and the presence of miR156 regulation elements. Nineteen orthologous groups of OsSPLs in Oryza were also identified, and most exons within those genes maintained constant length, whereas length of intron changed relatively. All groups were constrained by stronger purifying selection and diversified continually including alterative gene number, intron length, and miR156 regulation. Subsequently, cis-acting element analyses revealed the potential role of SPLs in wild rice, which might participate in light-responsive, phytohormone response, and plant growth and development. Our results shed light on that different evolutionary rates and duplication events might result in divergent evolutionary patterns in each lineage of SPL genes, providing a guide in exploring diverse function in the rice gene family among six closely related Oryza species

The alleviation of crowding effect through perceptual learning

PURPOSE: Our recent study showed that crowding effect can be completely eliminated by perceptual learning (Zhu, Fan, and Fang, JOV 2016). Here, we present data to further characterize this process. METHODS: Subjects were trained on a crowded orientation discrimination task with a target centered at 10&deg; eccentricity together with two abutting flankers positioned radially. The target and flankers were a circular patch of a sine-wave grating. Before and after training, we measured orientation discrimination thresholds with the crowded and isolated targets. In Experiment 1, the diameter of the target and flankers could be 1.5&deg;, 2&deg;, 2.5&deg; or 3&deg;. RESULTS: We found that the extent of alleviation of the crowding effect by training depended on the center-to-center distance between the target and flankers. The greater the distance, the less crowding effect after training. When the distance was larger than 3&deg;, the crowding effect can be completely eliminated. In Experiment 2, we first replicated our previous finding that there was little transfer of the learning effect between the left and right visual fields. A new finding is that the learning effect to eliminate crowding could completely transfer from the upper to the lower visual filed, but not vice versa. In Experiment 3, we examined whether the learned ability to eliminate the orientation crowding could generalize to eliminate letter crowding. Before and after training, we also measured the contrast thresholds for identifying crowded and isolated target letters, which had the same size as and were placed at the same location as the target grating. We found that the learning effect could completely transfer and eliminate the letter crowding effect. CONCLUSIONS: Taken together, these results suggest that, with a relative large target, crowding effect is dominated by some high-level cognitive components, though constrained by visual hemifield properties. The cognitive components can be modified by perceptual training.</p

Effect of Intraorbital Mechanical Compression on Retinal Microvascular Perfusion in Quiescent Thyroid-Associated Ophthalmopathy Based on Ocular Biomechanics Measured by Corvis ST

Abstract Introduction To analyze the correlation between orbital compliance and retinal vessel density (VD) based on dynamic Scheimpflug analyzer (Corvis ST) and optical coherence tomographic angiography (OCT-A). Methods In this prospective observational study, 65 eyes of 44 patients with thyroid-associated ophthalmopathy (TAO) in quiescent stage were included (15 males and 29 females). The whole eye movement (WEM) was detected by Corvis ST. The superficial capillary plexus VD (SCP-VD) and deep capillary plexus VD (DCP-VD) were obtained by scanning the 3 × 3 mm area around the fovea using OCT-A, while the peripapillary vessel density (ppVD) was obtained by scanning the 4.5 × 4.5 mm area around the optic disk. Covariances including biomechanically corrected intraocular pressure (bIOP), axial length, age and gender were adjusted during data analysis. Results The mean WEM of the participants was 0.235 ± 0.066 mm. The mean SCP-VD and DCP-VD in whole image were 46.20% ± 3.77% and 50.51% ± 3.96%; the mean whole pp-VD was 49.75% ± 2.01%. WEM was positively correlated with SCP-VD (r = 0.327, p = 0.01) and the whole pp-VD (r = 0.394, p < 0.01) after adjusting by gender, axial length (AL), age and bIOP, but it was not significantly correlated with DCP-VD (r = 0.072 p = 0.581). Conclusion Increase in orbital pressure might reduce retinal microvascular perfusion. Our data suggest orbital mechanical compression may be an important cause of retinal VD changes in quiescent patients with TAO

Simultaneous refinement of α-Al and modification of Si in Al–Si alloy achieved via the addition of Y and Zr

Due to the Si/Zr-poisoning effect, conventional refiners often yield unsatisfactory results in refining α-Al grains for Al–Si alloys. In this work, the refinement of α-Al grains and the modification of eutectic Si are simultaneously achieved through the combined addition of Y and Zr to A356 alloy. The results indicate that the individual addition of Zr aids in refinement due to the formation of heterogeneous nuclei Al3Zr, while Y alone can refine α-Al grains and modify eutectic Si to a certain extent. When Y and Zr are added together, not only is the size of α-Al grains further refined to 22.1 μm, but the morphology of eutectic Si is also completely transformed into a fibrous structure. The interaction between Zr and Y decreases their solubility in the matrix, enhances heterogeneous nucleation, and induces the formation of a Al8FeMg3Si6YxZry phase enriched at grain boundaries, suppressing the growth of eutectic Si flakes. Compared to the use of Al–Ti–B refiners and Sr modifiers, the alloy with 0.25Zr and 0.3Y additions exhibits more significant grain refinement and Si modification. Furthermore, it facilitates the precipitation of fine Al3(Y, Zr) particles during the T6 heat treatment process, resulting in an optimal ultimate tensile strength (UST) of 289 MPa and an elongation of 9.75%

Meta-Analysis of Salt Stress Transcriptome Responses in Different Rice Genotypes at the Seedling Stage

Rice (Oryza sativa L.) is one of the most important staple food crops worldwide, while its growth and productivity are threatened by various abiotic stresses, especially salt stress. Unraveling how rice adapts to salt stress at the transcription level is vital. It can provide valuable information on enhancing the salt stress tolerance performance of rice via genetic engineering technologies. Here, we conducted a meta-analysis of different rice genotypes at the seedling stage based on 96 public microarray datasets, aiming to identify the key salt-responsive genes and understand the molecular response mechanism of rice under salt stress. In total, 5559 genes were identified to be differentially expressed genes (DEGs) under salt stress, and 3210 DEGs were identified during the recovery process. The Gene Ontology (GO) enrichment results revealed that the salt-response mechanisms of shoots and roots were different. A close-knit signaling network, consisting of the Ca2+ signal transduction pathway, the mitogen-activated protein kinase (MAPK) cascade, multiple hormone signals, transcription factors (TFs), transcriptional regulators (TRs), protein kinases (PKs), and other crucial functional proteins, plays an essential role in rice salt stress response. In this study, many unreported salt-responsive genes were found. Besides this, MapMan results suggested that TNG67 can shift to the fermentation pathway to produce energy under salt stress and may enhance the Calvin cycle to repair a damaged photosystem during the recovery stage. Taken together, these findings provide novel insights into the salt stress molecular response and introduce numerous candidate genes for rice salt stress tolerance breeding

Dietary energy intake affects fetal survival and development during early and middle pregnancy in Large White and Meishan gilts

This experiment was designed to determine the effects of variations in dietary energy intake on reproductive performance and gene expression of luteal and endometrium tissues in Large White (LW) and Meishan (MS) gilts during early and middle pregnancy. After insemination, 32 LW gilts were assigned to high and low (HEL and LEL, 14.23 and 12.56 MJ DE/kg, respectively) diet treatment groups, while 32 MS gilts were allocated to HEM and LEM (12.56 and 10.88 MJ DE/kg) groups. Gilts were slaughtered on days 35, 55 and 90 of gestation. The fetal survival and luteal progesterone (P4) concentration in the HEL group were higher on day 35 but lower on day 90 of gestation compared with the LEL group (P < 0.05) for LW gilts. However, fetal survival and luteal P4 concentration on day 35 of gestation were greater (P < 0.05) in the LEM group than in the HEM group for MS gilts, but no significant difference in mid-gestation was showed. The fetal weights of both breeds were higher for the high energy diets compared with the respective control group on day 90 of gestation (P < 0.05). In addition, the mRNA levels of P4 synthesis-related proteins had correlated with luteal P4 concentration in both breeds. Further, endometrial levels of uteroferrin (ACP5), retinol-binding protein 4 (RBP4) and secreted phosphoprotein 1 (SPP1) mRNA were upregulated in the HEL group on day 35 of gestation but ACP5 and SPP1 were downregulated on day 55 of gestation compared with the LEL group (P < 0.05) for LW gilts. In MS gilts, diet only affected the expression of SPP1 (P < 0.05). Our results revealed the differential sensitivity of LW and MS breeds to variations in dietary energy intake. For LW gilts, the HEL group improved fetal survival on day 35 but a sustained high energy diet decreased fetal survival on day 90 of gestation. The differences in dietary energy intake did not influence fetal survival on day 90 of gestation but the higher energy diet did increase fetal weight in the MS breed compared with the lower energy intake diet. These results may be due to differential luteal secretion activity and endometrium gene expression in these two breeds. Keywords: Energy level, Fetal survival, Corpus luteum, Endometrium, pi