Structure basis for the unique specificity of medaka enteropeptidase light chain

Thermal stresses concern not renewed type of stresses, that is once having liberated, they cannot accumulate more. The estimation of purely thermoelastic contribution to a lithosphere stress state gives the additional information, allowing to predict the danger connected with such natural factors, as seismic and volcanic activity. Some theoretical thermoelastic problems for the geological environment of a difficult outline with non-uniform thermophysical characteristics are considered. The decision is received on the basis of a numerical finite elements method. Influence of the model fixation, the geometrical factor and boundary conditions on distribution of thermal stresses and dislocation is investigated. Computing experiments have shown, that the size of the maximum thermal stresses reaches 500 bar. The maximum values of vertical dislocation are reached by 90 m, and horizontal — 50 m. Neutral plane position are precisely defined. Термоупругие напряжения относятся к невозобновляемому типу напряжений, то есть, однажды высвободившись, напряжения не могут накапливаться вновь. Расчет термоупругого вклада в напряженное состояние литосферы дает дополнительную информацию, позволяющую оценить опасность, связанную с такими природными явлениями, как сейсмичность и вулканическая активность. Рассмотрено несколько теоретических моделей для геологической среды сложного очертания с неоднородными теплофизическими характеристиками. Решение получено на основе численного метода конечных элементов. Исследовано влияние «закрепления» модели, геометрического фактора, неоднородных граничных условий на распределение термоупругих напряжений и перемещений. Вычислительные эксперименты показали, что величина максимальных термоупругих напряжений достигает 500 б. Максимальные величины вертикальных перемещений не превышают 90 м, горизонтальных — 50 м. Положение нейтральной плоскости определяется точно. На основі методу скінченних елементів отримано детальний розподіл термопружних напружень і переміщень для неоднорідного геологічного середовища. Досліджено взаємний вплив геометрії середовища й неоднорідних граничних умов на розподіл термопружних напружень та переміщень

Identification of wheat stem rust resistance genes in wheat cultivars from Hebei province, China

Wheat stem rust is caused by Puccinia graminis f. sp. tritici. This major disease has been effectively controlled via resistance genes since the 1970s. The appearance and spread of new races of P. graminis f. sp. tritici (eg., Ug99, TKTTF, and TTRTF) have renewed the interest in identifying the resistance gene and breeding cultivars resistant to wheat stem rust. In this study, gene postulation, pedigree analysis, and molecular detection were used to determine the presence of stem rust resistance genes in 65 commercial wheat cultivars from Hebei Province. In addition, two predominant races 21C3CTHTM and 34MRGQM were used to evaluate the resistance of these cultivars at the adult-plant stage in 2021–2022. The results revealed that 6 Sr genes (namely, Sr5, Sr17, Sr24, Sr31, Sr32, Sr38, and SrTmp), either singly or in combination, were identified in 46 wheat cultivars. Overall, 37 wheat cultivars contained Sr31. Sr5 and Sr17 were present in 3 and 3 cultivars, respectively. Gao 5218 strong gluten, Jie 13-Ji 7369, and Kenong 1006 contained Sr24, Sr32, and Sr38, respectively. No wheat cultivar contained Sr25 and Sr26. In total, 50 (76.9%) wheat cultivars were resistant to all tested races of P. graminis f. sp. tritici in field test in 2021–2022. This study is important for breeding wheat cultivars with resistance to stem rust

LKB1 Differently Regulates Adipogenesis in Intramuscular and Subcutaneous Adipocytes through Metabolic and Cytokine-Related Signaling Pathways

Liver kinase B1 (LKB1) plays important and various roles in the differentiation and lipid metabolism of adipocytes. However, the current knowledge of the respective roles of LKB1 in subcutaneous fat (SCF) and intramuscular fat (IMF) adipocytes remains unclear. This study aimed to discover the different regulatory mechanisms of LKB1 in SCF and IMF adipocytes. We found that LKB1 overexpression inhibited adipogenesis in both SCF and IMF adipocytes, and SCF adipocytes were more sensitive to regulation by LKB1. Transcriptomics results showed that IMF adipocytes had many more differentially expressed genes (DEGs) than SCF adipocytes. Pathway analysis of the shared and distinct DEGs revealed that the main adipogenesis mechanism was similar between SCF and IMF adipocytes upon LKB1 overexpression, while regulatory and metabolic signaling pathways, such as MAPK, PPAR signaling pathways, were differently regulated by LKB1. Several cytokine-related pathways were only enriched in LKB1-overexpressing IMF adipocytes. Our study reveals different regulators and signaling pathways between SCF and IMF adipocytes under LKB1 overexpression, which may be potential targets to differentially control SCF and IMF deposition and improve our understanding of the regulatory mechanisms of IMF deposition

A diode-pumped high-repetition-frequency passively Q-switched Nd:LaMgAl11O19 laser

High-repetition-frequency Q-switched laser is realized through adopting a Nd:LaMgAl11O19 (Nd:LMA) disordered crystal as the gain material, a laser diode lasing at 796 nm as the pumped source, and a semiconductor saturable absorber mirror (SESAM) as the Q-switched device. The out-put characteristics are analyzed under using different transmittance T plane mirrors as an output coupler. Without adopting SESAM, the laser is operating at a CW state, and a relatively high transmittance is helpful for achieving high output power, slope efficiency and light-to-light efficiency. ForT = 7.5% and an absorbed power of 6.17 W, the output power arrives at its maximum of 1160 mW,and the corresponding slope efficiency and light-to-light efficiency are 20.71% and 18.78%, respectively. After introducing SESAM into the cavity, the laser operates at a passively Q-switched state, and the largest slope efficiency is 13.14% under T = 5.0%. Adopting five different output couplers, with the increase of the absorbed power, the pulse repetition frequencies, the pulse energies and the peak powers will ascend while the pulse widths will decline. The observed narrowest pulse width, the maximum pulse repetition frequency, the highest pulse energy and peak power are 1.745 μs, 175.88 kHz, 3.21 μJ and 1.84 W, respectively

BIM-Based Green Hospital Building Performance Pre-Evaluation: A Case Study

With ecological problems and energy crises intensifying today, greening is essential to sustainable development. Compared with other types of buildings, hospital buildings account for a relatively larger proportion of building energy consumption. In order to realize the rapid cycle optimization of a green hospital project in the design stage and improve the green grade of the building, a pre-evaluation Building Information Model (BIM) of green hospital building performance was established in this study. Firstly, the literature review and expert consultation established the building performance pre-evaluation index system for green hospitals. Then, BIM technology is taken to extract data needed for building a performance pre-evaluation system, and the Cloud Model and the Matter–Element Extension Theory are used to build models. The final green grade calculation is realized in MATLAB. Finally, the Maluan Bay Hospital is taken as an example to test the applicability and effectiveness of the proposed model. The results show that the green hospital building performance pre-evaluation model has advantages of simulation, cyclic optimization and fuzzy quantification, which can effectively guide the design and construction of a green hospital

Adsorption Mechanism and Electrochemical Characteristic of Methyl Blue onto Calcium Ferrite Nanosheets

A rapid combustion process was applied to prepare CaFe2O4 nanomaterials using CaBr2·xH2O and Fe(NO3)3·9H2O as raw materials and CaFe2O4 nanomaterials were characterized by SEM, TEM, VSM, XRD, and FTIR techniques. The results showed that the prepared nanomaterials had a sheet-like structure, and for larger adsorption capacity of dyes, CaFe2O4 nanosheets prepared at 700°C for 2 h with average grain size was 93.3 nm, a thickness of 8.4 nm, and the saturation magnetization of 8.15 emu/g were employed as adsorbate for the removal of methyl blue (MB). The adsorption performance of MB onto CaFe2O4 nanosheets was investigated; CaFe2O4 nanosheets displayed favorable adsorption capacity, and the adsorption conformed to the pseudo-second-order model and the Freundlich model, which demonstrated that the adsorption process of MB on CaFe2O4 nanosheets belonged to multilayer chemisorption process. When the pH value reached 3, the adsorption capacity of MB by CaFe2O4 nanosheets kept maximum value of 478.07 mg/g; and after 5 regenerations, the removal efficiency of MB was reduced to 59.06% of the first time. The electrochemical behavior of MB onto the nanosheets was evaluated through CV in conjunction with EIS. The CaFe2O4 nanosheets revealed a promising prospect for the adsorption of dyes