Time trends in TDR among HIV-1 infected patients between 2003 to 2013.

<p>Time trends in TDR among HIV-1 infected patients between 2003 to 2013.</p

Phylogenetic tree analysis from ART-naïve patients diagnosed between 2009–2011.

<p>122 sequences of HIV-1 gag-pol were used and the lengths were 1057 bp by using HXB2 as the reference genomic. Each reference sequence is labeled with the HIV-1 subtype, followed by country, year, sampling name, and accession number. Isolate ZTT0203 was not clustered with the basic reference subtypes/CRFs. The trees were constructed by the neighbor-joining method. Bootstrap values were calculated from 1,000 analyses, and bootstrap probabilities greater than 70% are indicated at the corresponding nodes of the tree. Open circle: homosexual contact, solid circle: heterosexual contact, open square: blood transmission, solid square: IDU, open triangle: unknown.</p

Bootscanning analysis of gag-pol sequences (4300 bp) using SimPlot 3.5.1.

<p>A, Bootscanning analysis of gag-pol sequences of kang 007a. B, Bootscanning analysis of gag-pol sequences of kang 190. C, Bootscanning analysis of gag-pol sequences of kang 253. D, Bootscanning analysis of gag-pol sequences of ztt 0203. E. Bootscanning analysis of gag-pol sequences of the representative CRF55_01B (CN.2011.GDDG318.JX574662). F, Bootscanning analysis of gag-pol sequences of kang 019as. G, Bootscanning analysis of gag-pol sequences of kang 140. H, The reference sequences (subtype B, CRF01_AE, CRF07_BC) were shown and subtype C, J was used as an outlier. Bootscanning analysis illustrated the recombination structures for CRF55_01B and the newly identified URFs. The closely related parental strains for the URFs were identified: CRF01_AE and CRF07_BC. The condition used for C: Window: 200 bp, Step: 20 bp, GapStrip: on, Reps: 100, Kimura (2-parameter), T/t: 2.0. Neighbor-joining.</p

Phylogenetic tree analysis from ART-naïve patients diagnosed between 2003–2008.

<p>77 sequences of HIV-1 gag-pol (1057 bp) were used and each reference sequence is labeled with the HIV-1 subtype, followed by country, year, sampling name, and accession number. The trees were constructed by the neighbor-joining method. Bootstrap values were calculated from 1,000 analyses, and bootstrap probabilities greater than 70% are indicated at the corresponding nodes of the tree. Open circle: homosexual contact, solid circle: heterosexual contact, open square: blood transmission, solid square: IDU, open triangle: unknown.</p

The levels of resistance to antiretroviral drugs from 16 individuals with TDR strains.

<p>The levels of resistance to antiretroviral drugs from 16 individuals with TDR strains.</p

The proportion of various routes of HIV transmission in Shaanxi province during four time periods: 2003–2005; 2006–2008; 2009–2011; 2012–2013.

<p>The proportion of various routes of HIV transmission in Shaanxi province during four time periods: 2003–2005; 2006–2008; 2009–2011; 2012–2013.</p

Phylogenetic tree analysis from ART-naïve patients diagnosed between 2012–2013.

<p>165 sequences of HIV-1 gag-pol were used and The lengths were 1057 bp by using HXB2 as the reference genomic. Each reference sequence is labeled with the HIV-1 subtype, followed by country, year, sampling name, and accession number. Isolates: kang007a, kang190, kang253, kang019as, kang140 were not clustered with the basic reference subtypes/CRFs. The trees were constructed by the neighbor-joining method. Bootstrap values were calculated from 1,000 analyses, and bootstrap probabilities greater than 70% are indicated at the corresponding nodes of the tree. Open circle: homosexual contact, solid circle: heterosexual contact, open square: blood transmission, solid square: IDU, open triangle: unknown.</p

The levels of CD4⁺ T cell count and viral load in CRF01_AE, non-CRF01_AE infected patients through different transmission routes.

<p>A, The levels of CD4⁺ T cell count in CRF01_AE and non-CRF01_AE infected patients through different transmission routes. A low level baseline CD4⁺ T cell count was found among the CRF01_AE-infected persons compared with the non-CRF01_AE patients infected through sexual transmission; B, The levels of HIV viral load in CRF01_AE and non-CRF01_AE infected patients through different transmission routes. the CRF01_AE group was associated with a higher viral load in patients who were infected through sexual transmission (homosexual contact and heterosexual contact); however, the viral load was not higher in the patients infected through blood transmission. The statistical significance was calculated using the two independent samples nonparametric tests (Man-Whitney U). *, <i>p</i><0.05; **, <i>p</i><0.01; ***, <i>p</i><0.001; ns, no significant.</p

Univariate analysis of factors associated with the presence of HIV-1 TDR.

<p>CI, confidence interval; OR, odds ratio.</p><p>Univariate analysis of factors associated with the presence of HIV-1 TDR.</p

The proportion of various subtype of HIV-1 in Shaanxi province during four time periods: 2003–2005; 2006–2008; 2009–2011; 2012–2013.

<p>The proportion of various subtype of HIV-1 in Shaanxi province during four time periods: 2003–2005; 2006–2008; 2009–2011; 2012–2013.</p