Univariate and multivariate analyses of overall survival of gastric adenocarcinoma patients.

<p>HR, hazard ratio; CI, confidence interval; ^a Numbers of cases in each group; * Statistically significant (<i>P</i><0.05).</p><p>Univariate and multivariate analyses of overall survival of gastric adenocarcinoma patients.</p

Kaplan-Meier survival curves of gastric adenocarcinoma patients (n = 513) after gastrectomy.

<p>The survival rate of patients in <i>PTPRD</i>-high group was significantly higher than that of the patients in the <i>PTPRD</i>-low group (log-rank test, P<0.001).</p

Correlation between <i>PTPRD</i> expression and clinicopathological parameters of 513 gastric adenocarcinoma cases.

a<p>^aNumbers of cases in each group. * Statistically significant (<i>P</i><0.05).</p><p>Correlation between <i>PTPRD</i> expression and clinicopathological parameters of 513 gastric adenocarcinoma cases.</p

<i>PTPRD</i> protein expression in gastric adenocarcinoma surgical specimens evaluated by immunohistochemistry.

<p>(A) Strong <i>PTPRD</i> staining was observed in noncancerous gastric mucosa. (B) Strong <i>PTPRD</i> staining in well-differentiated gastric cancer. (C) Weak <i>PTPRD</i> staining in moderately differentiated GC. (D) Negative <i>PTPRD</i> staining in poorly differentiated GC. (E) Immunostaining of GC and adjacent nontumorous tissues showing a sharp contrast of <i>PTPRD</i> staining intensity.</p

The mRNA expression of <i>PTPRD</i> in human primary gastric adenocarcinoma surgical specimens was evaluated by qRT-PCR.

<p>The relative mRNA expression of <i>PTPRD</i> was significantly decreased in GC tissues compared with the matched adjacent noncancerous tissues (n = 42, P = 0.0138). Horizontal lines represent the mean.</p

The growth suppressor role of <i>PTPRD</i> in cell proliferation and DNA methylation analysis of <i>PTPRD</i>.

<p>(A) Western blotting analysis of <i>PTPRD</i> overexpression in MGC803 cells. (B) Western blotting analysis of decreased <i>PTPRD</i> expression in GES1 cells. (C) Cell proliferation assay showing the suppressive effect of restoring <i>PTPRD</i> expression on the proliferation of MGC803 cell line. (D) Results showing significantly enhanced proliferation rate of <i>PTPRD</i>-silenced GES1 cells compared with mock siRNA treatment GES1 cells. (E) Methylation analysis of <i>PTPRD</i> promoter CpG island in primary GC tissues. Among 3 GC samples, one case showed partial methylation and 2 cases were unmethylated. *, P<0.05 versus the mock control; **, P<0.01 versus the mock control.</p