Glutamine in suppression of lipopolysaccharide-induced piglet intestinal inflammation: The crosstalk between AMPK activation and mitochondrial function

Publication history: Accepted - 18 March 2022; Published online - 26 March 2022.The study was conducted to investigate the regulatory mechanism of glutamine (Gln) on intestinal inflammation in an Escherichia coli lipopolysaccharide (E. coli LPS)-induced in vivo and in vitro models. Piglets (n = 8) weaned at 21 d of age were fed a basal diet (control and LPS groups) or 1% Gln diet (Gln + LPS group) ad libitum for 4 weeks. On d 22, 24, 26 and 28, piglets in the LPS and Gln + LPS groups were intraperitoneally injected with E. coli LPS. Intestinal porcine epithelial cells (IPEC-J2) (n = 6) induced by LPS were used to assess related mechanisms and compound C was used to inhibit adenosine 5′-monophosphate-activated protein kinase (AMPK) activity. Our current results showed that compared with the LPS treatment, the Gln + LPS treatment had better growth performance and greater villus height (P < 0.05), and the Gln + LPS treatment reduced the rate of diarrhea by 6.4% (P < 0.05); the Gln + LPS treatment decreased serum tumor necrosis factor (TNF-ɑ), interleukin-6 (IL-6), K+, cortisol and insulin levels, whereas increased (P < 0.05) serum immunoglobulin M and epidermal growth factor levels; the Gln + LPS treatment increased (P < 0.05) the expression of aquaporins and AMPK pathway-associated targets in the jejunum and ileum of piglets, whereas decreased the expression of ion transporters (P < 0.05). The in vitro results showed that 4 mmol/L Gln administration could inhibit (P < 0.05) cell apoptosis and interleukin-1β (IL-1β), IL-6 and TNF-ɑ secretion in LPS-induced IPEC-J2 cells, promote (P < 0.05) mitochondrial respiratory metabolism and increase (P < 0.05) the number of mitochondria and mitochondrial membrane potential. The activity of AMPK was elevated by 70% to 300% in Gln-treated IPEC-J2 cells under LPS challenge or normal conditions. Our results indicate that pre-administration of Gln to piglets suppresses intestinal inflammation by modulating the crosstalk between AMPK activation and mitochondrial function.This work was supported by Huxiang Young Talent Support Program (2020RC3052), Natural Science Foundation of China (31902168, 31872371), Hunan Key Research and Development Plan (2020NK2059), State Key Laboratory of Animal Nutrition (2004DA125184F1907), Special Funds for Construction of Innovative Provinces in Hunan Province (2019RS3022), Guangxi Key Research and Development Plan (Guike AB19259012), and Guangxi Guilin Science and Technology Planning Project (2020010901)

Potential of Core-Collapse Supernova Neutrino Detection at JUNO

JUNO is an underground neutrino observatory under construction in Jiangmen, China. It uses 20kton liquid scintillator as target, which enables it to detect supernova burst neutrinos of a large statistics for the next galactic core-collapse supernova (CCSN) and also pre-supernova neutrinos from the nearby CCSN progenitors. All flavors of supernova burst neutrinos can be detected by JUNO via several interaction channels, including inverse beta decay, elastic scattering on electron and proton, interactions on C12 nuclei, etc. This retains the possibility for JUNO to reconstruct the energy spectra of supernova burst neutrinos of all flavors. The real time monitoring systems based on FPGA and DAQ are under development in JUNO, which allow prompt alert and trigger-less data acquisition of CCSN events. The alert performances of both monitoring systems have been thoroughly studied using simulations. Moreover, once a CCSN is tagged, the system can give fast characterizations, such as directionality and light curve

Detection of the Diffuse Supernova Neutrino Background with JUNO

As an underground multi-purpose neutrino detector with 20 kton liquid scintillator, Jiangmen Underground Neutrino Observatory (JUNO) is competitive with and complementary to the water-Cherenkov detectors on the search for the diffuse supernova neutrino background (DSNB). Typical supernova models predict 2-4 events per year within the optimal observation window in the JUNO detector. The dominant background is from the neutral-current (NC) interaction of atmospheric neutrinos with 12C nuclei, which surpasses the DSNB by more than one order of magnitude. We evaluated the systematic uncertainty of NC background from the spread of a variety of data-driven models and further developed a method to determine NC background within 15\% with {\it{in}} {\it{situ}} measurements after ten years of running. Besides, the NC-like backgrounds can be effectively suppressed by the intrinsic pulse-shape discrimination (PSD) capabilities of liquid scintillators. In this talk, I will present in detail the improvements on NC background uncertainty evaluation, PSD discriminator development, and finally, the potential of DSNB sensitivity in JUNO

Real-time Monitoring for the Next Core-Collapse Supernova in JUNO

Core-collapse supernova (CCSN) is one of the most energetic astrophysical events in the Universe. The early and prompt detection of neutrinos before (pre-SN) and during the SN burst is a unique opportunity to realize the multi-messenger observation of the CCSN events. In this work, we describe the monitoring concept and present the sensitivity of the system to the pre-SN and SN neutrinos at the Jiangmen Underground Neutrino Observatory (JUNO), which is a 20 kton liquid scintillator detector under construction in South China. The real-time monitoring system is designed with both the prompt monitors on the electronic board and online monitors at the data acquisition stage, in order to ensure both the alert speed and alert coverage of progenitor stars. By assuming a false alert rate of 1 per year, this monitoring system can be sensitive to the pre-SN neutrinos up to the distance of about 1.6 (0.9) kpc and SN neutrinos up to about 370 (360) kpc for a progenitor mass of 30$M_{\odot}$ for the case of normal (inverted) mass ordering. The pointing ability of the CCSN is evaluated by using the accumulated event anisotropy of the inverse beta decay interactions from pre-SN or SN neutrinos, which, along with the early alert, can play important roles for the followup multi-messenger observations of the next Galactic or nearby extragalactic CCSN.Comment: 24 pages, 9 figure

Research and Design of a Routing Protocol in Large-Scale Wireless Sensor Networks

无线传感器网络，作为全球未来十大技术之一，集成了传感器技术、嵌入式计算技术、分布式信息处理和自组织网技术，可实时感知、采集、处理、传输网络分布区域内的各种信息数据，在军事国防、生物医疗、环境监测、抢险救灾、防恐反恐、危险区域远程控制等领域具有十分广阔的应用前景。 本文研究分析了无线传感器网络的已有路由协议，并针对大规模的无线传感器网络设计了一种树状路由协议，它根据节点地址信息来形成路由，从而简化了复杂繁冗的路由表查找和维护，节省了不必要的开销，提高了路由效率，实现了快速有效的数据传输。 为支持此路由协议本文提出了一种自适应动态地址分配算——ADAR（AdaptiveDynamicAddre...As one of the ten high technologies in the future, wireless sensor network, which is the integration of micro-sensors, embedded computing, modern network and Ad Hoc technologies, can apperceive, collect, process and transmit various information data within the region. It can be used in military defense, biomedical, environmental monitoring, disaster relief, counter-terrorism, remote control of haz...学位：工学硕士院系专业：信息科学与技术学院通信工程系_通信与信息系统学号：2332007115216

A Novel Approach to Oil Layer Recognition Model Using Whale Optimization Algorithm and Semi-Supervised SVM

The dataset distribution of actual logging is asymmetric, as most logging data are unlabeled. With the traditional classification model, it is hard to predict the oil and gas reservoir accurately. Therefore, a novel approach to the oil layer recognition model using the improved whale swarm algorithm (WOA) and semi-supervised support vector machine (S3VM) is proposed in this paper. At first, in order to overcome the shortcomings of the Whale Optimization Algorithm applied in the parameter-optimization of the S3VM model, such as falling into a local optimization and low convergence precision, an improved WOA was proposed according to the adaptive cloud strategy and the catfish effect. Then, the improved WOA was used to optimize the kernel parameters of S3VM for oil layer recognition. In this paper, the improved WOA is used to test 15 benchmark functions of CEC2005 compared with five other algorithms. The IWOA–S3VM model is used to classify the five kinds of UCI datasets compared with the other two algorithms. Finally, the IWOA–S3VM model is used for oil layer recognition. The result shows that (1) the improved WOA has better convergence speed and optimization ability than the other five algorithms, and (2) the IWOA–S3VM model has better recognition precision when the dataset contains a labeled and unlabeled dataset in oil layer recognition

Dynamic Multi-Swarm Differential Learning Quantum Bird Swarm Algorithm and Its Application in Random Forest Classification Model

Bird swarm algorithm is one of the swarm intelligence algorithms proposed recently. However, the original bird swarm algorithm has some drawbacks, such as easy to fall into local optimum and slow convergence speed. To overcome these short-comings, a dynamic multi-swarm differential learning quantum bird swarm algorithm which combines three hybrid strategies was established. First, establishing a dynamic multi-swarm bird swarm algorithm and the differential evolution strategy was adopted to enhance the randomness of the foraging behavior’s movement, which can make the bird swarm algorithm have a stronger global exploration capability. Next, quantum behavior was introduced into the bird swarm algorithm for more efficient search solution space. Then, the improved bird swarm algorithm is used to optimize the number of decision trees and the number of predictor variables on the random forest classification model. In the experiment, the 18 benchmark functions, 30 CEC2014 functions, and the 8 UCI datasets are tested to show that the improved algorithm and model are very competitive and outperform the other algorithms and models. Finally, the effective random forest classification model was applied to actual oil logging prediction. As the experimental results show, the three strategies can significantly boost the performance of the bird swarm algorithm and the proposed learning scheme can guarantee a more stable random forest classification model with higher accuracy and efficiency compared to others

Semi-Supervised Ensemble Classifier with Improved Sparrow Search Algorithm and Its Application in Pulmonary Nodule Detection

The Adaptive Boosting (AdaBoost) classifier is a widely used ensemble learning framework, and it can get good classification results on general datasets. However, it is challenging to apply the AdaBoost classifier directly to pulmonary nodule detection of labeled and unlabeled lung CT images since there are still some drawbacks to ensemble learning method. Therefore, to solve the labeled and unlabeled data classification problem, the semi-supervised AdaBoost classifier using an improved sparrow search algorithm (AdaBoost-ISSA-S4VM) was established. Firstly, AdaBoost classifier is used to construct a strong semi-supervised classifier using several weak classifiers S4VM (AdaBoost-S4VM). Next, in order to solve the accuracy problem of AdaBoost-S4VM, sparrow search algorithm (SSA) is introduced in the AdaBoost classifier and S4VM. Then, sine cosine algorithm and new labor cooperation structure are adopted to increase the global optimal solution and convergence performance of sparrow search algorithm, respectively. Furthermore, based on the improved sparrow search algorithm and adaptive boosting classifier, the AdaBoost-S4VM classifier is improved. Finally, the effective improved AdaBoost-ISSA-S4VM classification model was developed for actual pulmonary nodule detection based on the publicly available LIDC-IDRI database. The experimental results have proved that the established AdaBoost-ISSA-S4VM classification model has good performance on labeled and unlabeled lung CT images