Levantamento de rizóbios isolados de nódulos de caupi cultivado em amostras de solos do cerrado do Estado do Piauí.

Cultivo de plantas iscas. Isolamento de nódulos, carcterização dos isolados. Resultados e discussão. Referências bibliográficas. Anexo 1. Anexo 2.bitstream/CNPAB-2010/27392/1/doc125.pd

Cerebrovascular risk in restless legs syndrome: Intima-media thickness and cerebral vasomotor reactivity: A case\u2013control study

Purpose: Although some studies have suggested an association between cardiovascular disease and restless legs syndrome (RLS), the mechanisms underlying this relationship remain unclear. The intima-media thickness (IMT) and vasomotor reactivity are two simple, non-invasive tools to investigate preclinical atherosclerosis and microangiopathy, respectively. The aims of this study were to evaluate carotid IMT and to explore vasomotor reactivity in idiopathic RLS (iRLS) patients. Patients and Methods: We enrolled 44 iRLS after exclusion of patients with secondary causes of RLS, history of vascular events, known uncontrolled vascular risk factors and other neurological disorders. Forty-four age and sex matched controls were therefore recruited. No significant differences in demographic data and vascular risk factors were found between the two groups. Carotid IMT was measured with a high-resolution B-mode ultrasound on the far-wall of common carotid artery, 10 mm and 30 mm to the carotid bulb. Vasomotor reactivity to hypo-and hypercapnia was assessed, by right middle cerebral artery transcranial Doppler, accordingly to the changes in peak systolic velocity, peak diastolic velocity and mean blood flow velocity. Results: Mean IMT was significantly increased in patients with iRLS when measured immediately proximally to carotid bifurcation (0.73; sd=0.17), versus controls (0.65; sd=0.13); p=0.035. Patients showed higher cerebrovascular flow velocities (CBFVs) compared to controls. After multivariate analysis, age, hypertension and iRLS proved to be independent IMT predictors. Conclusion: Increased IMT and higher CBFVs in iRLS support the association of iRLS with vascular damage, possibly through enhanced atherogenesis and sympathetic hyperactivity. However, to clarify a causal relationship, further longitudinal assessment of these parameters is needed, trying to control all their physiological modifying factors

APPLICATION OF DUPLEX STAINLESS STEEL FOR WELDED BRIDGE CONSTRUCTION IN AGGRESSIVE ENVIRONMENT

Maintenance costs are a significant item in life cycle of steel bridges, becoming of paramount importance in aggressive environments. The use of duplex stainless steels for bridge decks would be a major step forward in providing durable, low maintenance structures, exploiting both their corrosion resistance and high mechanical properties, capable of meeting in full the required structural safety performances. A research project partially funded by the EU research programme RFCS (Research Fund for Coal and Steel, Bridgeplex contract RFS-CR-04040) is developing technical information on the use of duplex stainless steel in welded bridge construction via mechanical testing and numerical analyses, so as to provide indications suitable to form the basis for an upgrade of Eurocode 3 [1] and to allow a reliable Life Cycle Cost analysis for this kind of structures so as to address the best material choice for the future bridges. The project is still in progress but first results are available. This paper gives an overview of the project and summarizes results obtained, deeper detailed in other papers presented at the International Conference Duplex 2007 ([5] and [6]). In particular the paper is concerned with: · overview of critical details in a welded bridge deck and relevant data available in literature also on austenitic and austeno-ferritic steels; and · economical evaluations considering maintenance aspects and fabrication costs showing the advantages of the application of duplex stainless steel to defined bridge typologies

Recomendação de inoculante para cultura de milho utilizando a bactéria Herbaspirillum seropedicae, estirpe BR11417

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Soil characteristics determine the rhizobia in association with different species of Mimosa in central Brazil.

Background and aims: To evaluate the influence of soil type on the symbiosis between Mimosa spp. and rhizobia. Methods: A greenhouse experiment was carried out with trap plants using seeds of six species of Mimosa and soils from three different locations in central Brazil: Posse, Brasília and Cavalcante. Plant dry biomass and number of nodules were measured after four months. Symbiotic bacteria were isolated from nodules and their molecular identification was performed. Three housekeeping genes (16S rRNA, recA and gyrB) plus the nodC and nifH symbiotic genes were used to determine the identity of the symbionts and to reconstruct the phylogenetic relationships among the isolated nitrogen-fixing bacteria. Results: Rhizobia from the Betaproteobacterial genus Paraburkholderia (former Burkholderia) and the Alphaproteobacterial genus Rhizobium were isolated from different species of Mimosa. As in previous studies, the phylogenies of their symbiosis-essential genes, nodC and nifH, were broadly congruent with their core housekeeping genes (16S rRNA, recA and gyrB), which suggests limited or no horizontal gene transfer. Edaphic factors such as pH and fertility influenced the occurrence of these unrelated rhizobial types in the nodules on these Mimosa spp. Conclusions: Mimosa species have the ability to associate with different types of rhizobia (?- and ?-proteobacteria), suggesting low specificity between host and bacterium in experimental conditions. Soil factors such as pH, nitrogen and fertility seem to favour the predominance of certain types of rhizobia, thus influencing the establishment of symbiotic relationships. © 2017 Springer International Publishing AG, part of Springer NatureMade available in DSpace on 2018-01-31T23:58:01Z (GMT). No. of bitstreams: 1 Zillisoilcharacteristicsdetermine5.pdf: 4439951 bytes, checksum: 10b93e8a39800192a1c492f0192bc020 (MD5) Previous issue date: 2018-01-30bitstream/item/171974/1/Zilli-soil-characteristics-determine-5.pd

Eficiência simbiótica de estirpes de Bradyrhizobium isoladas de solo do Cerrado em caupi.

O objetivo deste trabalho foi avaliar as relações filogenéticas de estirpes de Bradyrhizobium e a contribuição destas estirpes para a fixação biológica de nitrogênio em caupi, em solos do Cerrado. Na avaliação da relação filogenética, o gene 16S rDNA de cada uma das estirpes foi amplificado e seqüenciado, e para a análise da eficiência simbiótica, determinou-se: N total, matéria seca das plantas, massa de nódulos e redução de acetileno, em casa de vegetação, e ocupação nodular, em experimento de campo. A maioria das estirpes estudadas pertence a B. elkanii e, pelo menos dez das estirpes, independentemente da espécie, apresentaram bom desempenho quanto à fixação biológica de N2. As estirpes BR3262, BR3280 (caracterizadas como B. elkanii) e BR3267, BR3287 e BR3288 (Bradyrhizobium sp.) mostram-se como inoculantes potenciais para o caupi, em razão do bom desempenho tanto na eficiência simbiótica quanto na ocupação nodular