General characteristics of the study participants.

<p>Data are presented as means and SD, counts, or medians and interquartile ranges, as appropriate. HOMA-IR: homeostasis model of insulin resistance; HDL: high-density lipoprotein; LDL: low-density lipoprotein. <i>P</i> values are presented for comparison between GDM subjects and control subjects. The characteristics of the PCOS group are shown.</p

Relationships of FGF19 and FGF21 with clinical, anthropometric and biochemical parameters calculated in a combined population of healthy pregnant controls and patients with GDM (n = 90).

<p>The correlations were calculated in the combined population of GDM patients and controls. Units and abbreviations are as <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081190#pone-0081190-t001" target="_blank">Table 1</a>.</p><p>*Significant correlation as assessed by Spearman correlation method.</p

Scattergram of serum FGF21 levels in patients with PCOS history, GDM patients without PCOS history.

<p>Horizontal lines across the scatter diagram represent median values. Differences between groups were assessed by Mann-Whitney <i>U</i> test with Bonferroni adjustment for multiple testing.</p

Scattergram of serum FGF19 levels in patients with PCOS history, GDM patients without PCOS history.

<p>Horizontal lines across the scatter diagram represent median values. Differences between groups were assessed by Mann-Whitney <i>U</i> test with Bonferroni adjustment for multiple testing.</p

Multiple linear regression analysis between FGF19 (dependent variable) and age, BMI, HOMA-IR, adiponectin, and PCOS history, as well as between FGF21 (dependent variable) and age, BMI, HOMA-IR, adiponectin and TG.

<p>β Coefficients and P values are given. Abbreviations are indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081190#pone-0081190-t001" target="_blank">Table 1</a>.</p><p>*Significant correlation.</p

IFI6 inhibits apoptosis of VECs induced by DENV2.

<p>(a and b) Apoptosis of IFI6+/+ cells, IFI6-/- cells and vector cells were detected using TUNEL assay after 24, 36, and 48 hrs post DENV2 infection.(c) Apoptosis of IFI6+/+ cells, IFI6-/- cells and vector cells were detected using AnnexinV-FITC/PI labeling flow cytometry after 24, 36, and 48 hrs post DENV2 infection.(d) Analysis of AnnexinV positive cell ratio.(e and f) Cell viability was determined by MTT. ***P<0.01.</p

IFI6 influences the expressions of XAF1, bcl-2 and bax.

<p>(a) Recombinant cells were infected by DENV2 (MOI = 4) for 24, 36 and 48 hrs. Immunoblotting was used to detect the expression of XAF1, Bcl-2 and Bax in IFI6 over-expression or knock-down cell lines. (b) Quantitative data in panel a. The results shown were from three independent experiments.</p

Changes in mitochondrial membrane potential (Δψm) in infected recombinant VECs.

<p>Δψm was measured by fluorescence microscopy in vector-hi/IFI6+/+ cells (a) and vector-low/IFI6-/- cells (b) stained by JC-1 after 24, 36 and 48 hrs post DENV2 infection. Scale bar = 50 μm.</p

Identification of recombinant vascular endothelial EA.hy926 cell lines with stable over-expression/knock-down of IFI6.

<p>(a and b) mRNA expression of IFI6 was detected using qRT-PCR in EA.hy926 cells with vector-hi(a), vector-low(b) and the recombinant cell lines IFI6+/+ (a) and IFI6-/- (b). (c and d) Protein expression of IFI6 was detected using immunoblotting in the indicated lysate (c) and the gray scale scanning data were shown below and normalized to β-actin (d). ***P<0.01.</p

Dengue virus infection induced IFI6 over-expression in isolated primary HUVECs and EA.hy926 cells.

<p>Expression of IFI6 was detected by GeneChip hybridization and analyzed(see Panel a, top) and mRNA expression of IFI6 was detected using qRT-PCR (see Panel a, below) at 48 hrs post-infection in HUVECs. Factor VIII antigen staining by immunocytochemistry (×400)(see Panel b, top). Analysis of CD31 expression in EA.hy926 cells by flow cytometry. The red was the negative control(see Panel b, below). (c) Protein expression of IFI6 was detected using immunoblotting in EA.hy926 cells after 24, 36, and 48 hrs post DENV2 infection. The gray scale scanning data were shown below and normalized to β-actin.</p