Expression and function of ATP-dependent potassium channels in zebrafish islet β-cells

ATP-sensitive potassium channels (K(ATP) channels) are critical nutrient sensors in many mammalian tissues. In the pancreas, K(ATP) channels are essential for coupling glucose metabolism to insulin secretion. While orthologous genes for many components of metabolism–secretion coupling in mammals are present in lower vertebrates, their expression, functionality and ultimate impact on body glucose homeostasis are unclear. In this paper, we demonstrate that zebrafish islet β-cells express functional K(ATP) channels of similar subunit composition, structure and metabolic sensitivity to their mammalian counterparts. We further show that pharmacological activation of native zebrafish K(ATP) using diazoxide, a specific K(ATP) channel opener, is sufficient to disturb glucose tolerance in adult zebrafish. That β-cell K(ATP) channel expression and function are conserved between zebrafish and mammals illustrates the evolutionary conservation of islet metabolic sensing from fish to humans, and lends relevance to the use of zebrafish to model islet glucose sensing and diseases of membrane excitability such as neonatal diabetes

A Study on Factors Affecting Sleep during Pregnancy in Clinical Trials

Regular sleep is required for sensory processing, learning, and brain plasticity. During pregnancy, poor sleep quality and dysregulation of hormones are all associated with increased risk for diseases like postpartum major depression[1]. Seventy-eight percent of pregnant women experience sleep problems at some point during pregnancy according to the National Sleep Foundation\u27s 1998 Women and Sleep poll. Chronodisruption is a frequent sleep disturbance experienced by pregnant women that can be primary or due to co-morbid conditions[2]. For this reason, chronodisruption, which includes insomnia, is currently regarded as one of the most important factors determining pregnancy outcome. Therefore, the goal of this study is to find essential factors to model effects of midpoint time of sleep during different trimesters as a measure of sleep quality. In this study, we are going to focus on sleep changes during pregnancy. Our underlying hypothesis is that circadian rhythms in the mother, fetus, or both regulate timing of parturition and, when disrupted, lead to preterm birth. I used linear regression models to address sleep changes during all three trimesters grouped by weekend and weekdays. Relationships between factors were investigated via correlation analysis. Interactions between melatonin/cortisol peak values and other factors such as sleep medication taken, vitamins taken, whether sleep was achieved within 30 min, and workload factors were explored. Other factors of interest such as race, having a paid job, and whether or not subjects had a night shift were investigated for overall midpoint sleep time as well as interactions with vitamins taken. Graphs were generated for models as well as for group comparisons. Correlation analysis, ANOVA, and linear regression methods were used to identify the most effective variable and to explain as much of the variance as possible. Factors affecting sleep midpoint and sleep hormones such as workload, sleep medicine taken, and prior pregnancy were successfully selected for non-pregnancy and all three pregnant periods for regression models. Model selection was based on the best adjusted R-squared evaluation metric. More details are discussed

Properties of Dynamical Black Hole Entropy

We study the first law for non-stationary perturbations of a stationary black hole whose event horizon is a Killing horizon, that relates the first-order change in the mass and angular momentum to the change in the entropy of an arbitrary horizon cross-section. Recently, Hollands, Wald and Zhang [1] have shown that the dynamical black hole entropy that satisfies this first law, for general relativity, is $S_{\text{dyn}}=(1-v\partial_v)S_{\text{BH}}$, where $v$ is the affine parameter of the null horizon generators and $S_{\text{BH}}$ is the Bekenstein-Hawking entropy, and for general diffeomorphism covariant theories of gravity $S_{\text{dyn}}=(1-v\partial_v)S_{\text{Wall}}$, where $S_{\text{Wall}}$ is the Wall entropy. They obtained the first law by applying the Noether charge method to non-stationary perturbations and arbitrary cross-sections. In this formalism, the dynamical black hole entropy is defined as an "improved" Noether charge, which is unambiguous to first order in the perturbation. In the present article we provide a pedagogical derivation of the physical process version of the non-stationary first law for general relativity by integrating the linearised Raychaudhuri equation between two arbitrary horizon cross-sections. Moreover, we generalise the derivation of the first law in [1] to non-minimally coupled matter fields, using boost weight arguments rather than Killing field arguments, and we relax some of the gauge conditions on the perturbations by allowing for non-zero variations of the horizon Killing field and surface gravity. Finally, for $f(\text{Riemann})$ theories of gravity we show explicitly using Gaussian null coordinates that the improved Noether charge is $S_{\text{dyn}}=(1-v\partial_v)S_{\text{Wall}}$, which is a non-trivial check of [1].Comment: 57 pages, 6 figures, 3 table

Optimization and stability analysis of the cascaded EEHG-HGHG free-electron laser

X-ray free-electron lasers (XFELs) are powerful tools to explore and study nature for achieving remarkable advances. Generally, seeded FELs are ideal sources for supplying full coherent soft x-ray pulses. Benefiting from the high-frequency up-conversion efficiency, the cascading configuration with echo-enabled harmonic generation (EEHG) and high-gain harmonic generation (HGHG) holds promising prospects for generating full coherent radiation at 1 nm wavelength. In this paper, we design and optimize EEHG-HGHG configuration using parameters of Shanghai High-Repetition-Rate XFEL and Extreme Light Facility. In addition, we systematically analyze the effect of relative timing jitter on the output FEL performance based on various start-to-end electron beams. The intensive numerical simulations show that the cascaded EEHG-HGHG scheme can achieve 1 nm FEL pulses with peak power up to 15 GW. Further sensitivity analysis indicates that the relative timing jitter between the electron beam and seed laser has a significant impact on the FEL performance. The RMS timing jitter of 3 fs can lead to the final output pulse energy fluctuations of 29.16%.Comment: 21 pages, 12 figure

Locally Spontaneous Dynamic Oxygen Migration on Biphenylene: A DFT Study

The dynamic oxygen migration on the interface of carbon materials, such as graphene and carbon nanotube, has opened up a new avenue to realizing the dynamic covalent materials. However, the understanding of dynamic behaviors of oxygen groups on the non-honeycomb structure, such as the biphenylene sheet, is still limited. Using both density functional theory calculations and ab initio molecular dynamics simulations, we demonstrate that the oxygen groups on the biphenylene, which is an allotrope of graphene and composed of four-, six- and eight-membered rings with unequal C-C bonds, can exhibit locally spontaneous dynamic oxygen migration through the breaking/reforming of the C-O bond. The density of state analyses show that the p-band center of the oxygen atom is closer to the Fermi energy level on biphenylene, compared to that of the oxygen atom adsorbed on graphene. This contrast confirms the locally spontaneous dynamic activity of the oxygen atom on biphenylene. This work provides scientific guidance for the exploration of the locally/globally spontaneous dynamic covalent materials and adds a new member to the 2D dynamic covalent material family.Comment: 13 pages, 4 figure

Inaudible Adversarial Perturbation: Manipulating the Recognition of User Speech in Real Time

Automatic speech recognition (ASR) systems have been shown to be vulnerable to adversarial examples (AEs). Recent success all assumes that users will not notice or disrupt the attack process despite the existence of music/noise-like sounds and spontaneous responses from voice assistants. Nonetheless, in practical user-present scenarios, user awareness may nullify existing attack attempts that launch unexpected sounds or ASR usage. In this paper, we seek to bridge the gap in existing research and extend the attack to user-present scenarios. We propose VRIFLE, an inaudible adversarial perturbation (IAP) attack via ultrasound delivery that can manipulate ASRs as a user speaks. The inherent differences between audible sounds and ultrasounds make IAP delivery face unprecedented challenges such as distortion, noise, and instability. In this regard, we design a novel ultrasonic transformation model to enhance the crafted perturbation to be physically effective and even survive long-distance delivery. We further enable VRIFLE's robustness by adopting a series of augmentation on user and real-world variations during the generation process. In this way, VRIFLE features an effective real-time manipulation of the ASR output from different distances and under any speech of users, with an alter-and-mute strategy that suppresses the impact of user disruption. Our extensive experiments in both digital and physical worlds verify VRIFLE's effectiveness under various configurations, robustness against six kinds of defenses, and universality in a targeted manner. We also show that VRIFLE can be delivered with a portable attack device and even everyday-life loudspeakers.Comment: Accepted by NDSS Symposium 202

A Comment on Deriving the Gibbons-Hawking-York Term From the String Worldsheet

In this note, we show that the noncovariant metric boundary term obtained from the nonlinear sigma model worldsheet derivation of the bulk off-shell sphere partition function is closely related to the Einstein boundary term in the Gamma-Gamma noncovariant action. In fact, when expressed in terms of the trace of the extrinsic curvature tensor, we illustrate that this boundary term has one-half the coefficient of the Gibbons-Hawking-York boundary term required such that the total (bulk plus boundary) off-shell classical action has a well-posed variational principle with Dirichlet boundary conditions.11 page

Cognitive deficits and impaired hippocampal long-term potentiation in K ATP-induced DEND syndrome

ATP-sensitive potassium (

Glass and glass ceramic electrodes and solid electrolyte materials for lithium ion batteries: A review

Due to its distinct network structure, lack of a grain boundary, and isotropic qualities, glass has been the subject of extensive research. Lithium ion batteries can have their capacity and safety increased by using glassy electrode and electrolyte materials. We discuss the properties and uses of several types of glass and glass ceramic as anodes, including tin oxide glass, vanadium oxide glass, and so on. Metal-organic framework (MOF) materials are also investigated as a new generation of high-performance anode materials. We present the usage of glassy MOF materials to overcome MOF material volume change during charge and discharge, as well as the order and disorder transition of certain MOF materials during charge and discharge. The use of vanadium-based glass as a cathode material is also discussed. These materials have the potential to be employed as electrode materials in the next generation of lithium- ion batteries. In addition, the application of glass, especially sulfide glass, as an all-solid-state battery electrolyte and the effect of mixed anion effect on improving the conductivity of solid electrolyte were introduced.</p

Probing new physics with multi-vacua quantum tunnelings beyond standard model through gravitational waves

We report on a novel phenomenon of particle cosmology, which features specific cosmological phase transitions via quantum tunnelings through multiple vacua. The latter is inspired by the axiverse ideas and enables us to probe the associated new physics models through a potential observation of specific patterns in the stochastic gravitational waves background. Multiple vacua may induce the nucleation of co-existing bubbles over the phase transition epoch, hence enhancing the overall process of bubbles' nucleation. Our detailed analysis of semi-analytical and numerical solutions to the bounce equations of the path integral in three vacua case has enabled us to determine the existence of three instanton solutions. This new mechanism of cosmological phase transitions clearly predicts a possibly sizeable new source of gravitational waves, with its energy spectrum being featured with particular patterns, which could be probed by the future gravitational wave interferometers.Comment: 8 pages, 2 figures, PLB accepted, Typos correcte