58 research outputs found
The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases.
yesSunburn is a commonly occurring acute inflammatory process, with dermal vasodilatation and leukocyte infiltration as central features. Ultraviolet (UV) B-induced hydrolysis of membrane phospholipids releases polyunsaturated fatty acids and their subsequent metabolism by cyclooxygenases (COX) and lipoxygenases (LOX) may produce potent eicosanoid mediators modulating different stages of the inflammation. Our objective was to identify candidate eicosanoids formed during the sunburn reaction in relation to its clinical and histological course. We exposed skin of healthy humans (n=32) to UVB and for 72h examined (i) expression of pro- and anti-inflammatory eicosanoids using LC/ESI-MS/MS and (ii) immunohistochemical expression of COX-2, 12-LOX, 15-LOX and leucocyte markers, while (iii) quantifying clinical erythema. We show that vasodilatory prostaglandins (PG)E2, PGF2¿ and PGE3 accompany the erythema in the first 24-48h, associated with increased COX-2 expression at 24h. Novel, potent leukocyte chemoattractants 11-, 12- and 8-monohydroxy-eicosatetraenoic acid (-HETE) are elevated from 4-72h, in association with peak dermal neutrophil influx at 24h, and increased dermal CD3+ lymphocytes and 12- and 15-LOX expression from 24-72h. Anti-inflammatory metabolite 15-HETE shows later expression, peaking at 72h. Sunburn is characterized by overlapping phases of increases in COX products followed by LOX products that may regulate subsequent events and ultimately its resolution.The Wellcome Trus
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Arachidonic acid metabolism by rat chloroleukemia cells in culture
(Omega-6 Essential Fatty Acids: Pathophysiology and Roles in Clinical Medicine, p127-p144)Modulation of tissue fatty acid composition, prostaglandin production and cholesterol levels by dietary manipulation of n-3 and n-6 essential fatty acid metabolites
Glucose‐6‐phosphate dehydrogenase is posttranslationally regulated in the larvae of the freeze‐tolerant gall fly, Eurosta solidaginis
The effect of leukotriene B5 on the accumulation of polymorphonuclear leukocytes (PMN) in normal skin and LTB4-stimulated human skin
ULTRAVIOLET RADIATION STIMULATES THE RELEASE OF ARACHIDONIC ACID FROM MAMMALIAN CELLS IN CULTURE
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