µFTIR maps obtained with calcium phosphate apatite features were collected for samples: PiCa, PiCa Mg2, PiCa Mg5, Pi4, Pi4 Mg2, Pi4 Mg5.

<p>The scale bar corresponds to 500 µm while the amplitude is between 0 and 10. Due to extended acquisition times, some maps were divided in 2 areas. The map size is 2000 µm x 4500 µm.</p

Flow chart for sample selection (RRT: Renal replacement therapy).

<p>Relative to the excluded patients, the included patients did not differ significantly in terms of gender and the prevalence of congestive heart failure but were older (+1 year, p<0.001), had a slight lower eGFR at initiation (-0.2ml/min, p = 0.03), were less likely to have diabetes (-2%, p<0.002) and had different patterns of initially treated conditions or primary renal disease (Table A in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0176616#pone.0176616.s001" target="_blank">S1 File</a>).</p

(A) SEM cartography of sample Pi4 Mg2 obtained with a magnification of 400 at 1 KeV (B) spatial distribution of Pi (purple maps), spatial distribution of Ca2+ (red maps) as obtained by EDX and SEM cartography (right pictures) obtained at 12 KeV for samples Pi4, Pi4 Mg2 and Pi4 Mg5.

<p>(A) SEM cartography of sample Pi4 Mg2 obtained with a magnification of 400 at 1 KeV (B) spatial distribution of Pi (purple maps), spatial distribution of Ca2+ (red maps) as obtained by EDX and SEM cartography (right pictures) obtained at 12 KeV for samples Pi4, Pi4 Mg2 and Pi4 Mg5.</p

Multivariate associations (hazard ratios and 95% confidence intervals derived from joint models) for access to transplantation for BMI in different multivariate joint models stratified on three levels of initial BMI with competing risk of death.

<p>Multivariate associations (hazard ratios and 95% confidence intervals derived from joint models) for access to transplantation for BMI in different multivariate joint models stratified on three levels of initial BMI with competing risk of death.</p

Identification of the samples studied through µFTIR experiments.

<p>Samples nomenclature</p><p><b>Ct</b> = samples incubated in 1% FBS DMEM</p><p><b>Pi3</b> = samples incubated in 1% FBS DMEM with Pi set to 3 mM</p><p><b>Pi4</b> = samples incubated in 1% FBS DMEM with Pi set to 4 mM</p><p><b>Pi3 Mg2</b> = incubated in 1% FBS DMEM with Pi set to 3 mM and Mg²⁺ set to 2 mM</p><p><b>Pi4 Mg1.5</b> = incubated in 1% FBS DMEM with Pi set to 4 mM and Mg²⁺ set to 1.5 mM</p><p><b>Pi4 Mg2</b> = incubated in 1% FBS DMEM with Pi set to 4 mM and Mg²⁺ set to 2 mM</p><p><b>Pi4 Mg5</b> = incubated in 1% FBS DMEM with Pi set to 4 mM and Mg²⁺ set to 5 mM</p><p><b>PiCa</b> = samples incubated in 1% FBS DMEM with Pi set to 3 mM and Ca²⁺ set to 2.4 mM</p><p><b>PiCa Mg1.5</b> = samples incubated in 1% FBS DMEM with Pi set to 3 mM, Ca²⁺ set to 2.4 mM and Mg²⁺ set to 1.5 mM</p><p><b>PiCa Mg2</b> = samples incubated in 1% FBS DMEM with Pi set to 3 mM, Ca²⁺ set to 2.4 mM and Mg²⁺ set to 2 mM</p><p><b>PiCa Mg5</b> = samples incubated in 1% FBS DMEM with Pi set to 3 mM, Ca²⁺ set to 2.4 mM and Mg²⁺ set to 5 mM</p><p>It is of note that these experiments were conducted concomitantly with and without cells. The table is indicating the presence of apatite in experiments with cells. Data of passive Ca / Pi deposition on MirrIR slides are discussed in a proper section.</p><p>Identification of the samples studied through µFTIR experiments.</p

Typical optical image and mapping (scale from blue to red with increasing concentration), and FTIR spectra of crystals generated in Pi 4 or PiCa samples without or in presence of a total concentration of 2 mM of Mg²⁺.

<p>Ia) Optical image, and corresponding Ib) typical FTIR spectra, Ic) Optical image and corresponding Id) typical FTIR spectra of sample PiCa. IIa) Optical image and corresponding IIb) typical FTIR spectra, IIc) Optical image and corresponding IId) typical FTIR spectra of sample PiCa Mg2.</p

Multivariate associations (hazard ratios and 95% confidence intervals derived from joint models) for overall mortality for BMI in different multivariate joint models stratified on three levels of initial BMI.

<p>Multivariate associations (hazard ratios and 95% confidence intervals derived from joint models) for overall mortality for BMI in different multivariate joint models stratified on three levels of initial BMI.</p

Patient characteristics according to the BMI level at start of dialysis.

<p>Patient characteristics according to the BMI level at start of dialysis.</p

Multivariate associations between body mass index (BMI) at the start of dialysis and access to kidney transplantation.

<p>*Adjusted for gender, age, congestive heart failure, diabetes, chronic respiratory disease, coronary heart disease, cardiac dysrhythmia, peripheral vascular disease, active cancer disease and stroke.</p

Multivariate Cox regression analysis of risk factors at baseline for all-cause mortality and first non-fatal CV events.

<p>IMT: intima media thickness; PWV: pulse wave velocity; RR: risk ratio; CI: confidence interval. TRFs (traditional risk factors): age (in 1-year increments), gender, diabetes, triglyceride level (in 1 mmol/l increments), hypertension, and smoking status. Coronary calcification severity was considered in 100 AU increments.</p><p>Multivariate Cox regression analysis of risk factors at baseline for all-cause mortality and first non-fatal CV events.</p