Repair of endonuclease- and transposon-induced DNA double strand breaks by host factors in Arabidopsis

The maize Ac/Ds transposable elements are members of the hAT transposon family. Ac/Ds transpose in diverse species and via a mechanism similar to that involved in V(D)J recombination. Here, we studied the repair of Ds transposition-induced hairpins by host factors in Arabidopsis. The results indicate that AtKu could position hairpin-opening to sites close to the hairpin apex. In the absence of AtMre11 or AtKu70, repair junctions have a higher frequency of small deletions (3-10bp) and larger deletions (11-50bp), respectively. Comparison of the repair of I-SceI- and Ds-induced DSB indicates that Ac transposase could influence Ds-induced DSB repair. In addition, analysis of I-SceI-induced DSB repair indicates that both AtKu70 and AtMre11 are critical for non-homologous end joining (NHEJ); AtKu70, but not AtMre11 affects its fidelity. In these respects, NHEJ in Arabidopsis resembles that in S. cerevisiae. However, in Arabidopsis, the repair junctions are error-prone, and microhomology-mediated end joining is not affected by AtKu70. Hence, its junction patterns are similar to those in mammals. These results indicate the dynamics of DSB repair among different organisms

Study on dynamic characteristics of double cylinder double acting bilge pump transmission

In view of the complex structure of the ship bilge pump, the excitation source in the process of work is difficult to accurately determine the fault condition of the bilge pump through the vibration test data. Based on the theory of multi - body dynamics, rotor dynamics and electrical mechanics, the kinematics and kinetic equations of the bilge pump drive are established, and the dynamics, kinematics and vibration characteristics of bottom Pump drive analysis of the cabin is carried out. The frequency of the excitation force corresponding to the transmission mechanism is deduced, and the frequency of the excitation force of the main transmission is calculated

Long-term exposure to PM2.5 and fasting plasma glucose in non-diabetic adolescents in Yogyakarta, Indonesia

Background: Indonesia is facing serious air pollution. However, very few studies have been conducted to examine the health risks of air pollution in Indonesia, particularly for adolescents. Objective: To assess the association between long-term exposure to ambient particles with a diameter of <2.5 mm (PM2.5) and fasting plasma glucose (FPG) in adolescents. Methods: A cross-sectional study was conducted in 482 adolescents aged 14e18 years in Yogyakarta, Indonesia in 2016. We finally included 469 (97.30%) participants who had no missing data for data analysis. We collected individual data on socio-demographics, behavioral habits, and health information through standardized questionnaires. Satellite-based PM2.5 concentrations from 2013 to 2016 were assigned based on participants’ residential addresses. The association between PM2.5 and FPG was examined using a generalized linear regression model while FPG was modeled as a continuous variable. An ordered logistic regression model was used to assess the relationship between PM2.5 and FPG categories. Results: Every 1 mg/m3 increase in PM2.5 was associated with a 0.34 mg/dL [95 confidence interval (95% CI): 0.08 mg/dL, 0.59 mg/dL] increase in FPG levels. Comparing with the low FPG level (under 86 mg/dL), every 1 mg/m3 increase in PM2.5 was associated with a 10.20% (95% CI: 1.60%, 19.80%) increase in the odds of impaired fasting glucose (IFG) (100e125 mg/dL). Stratified analyses indicated greater effects on participants with hypertension [odds ratio (OR) ¼ 1.30, 95% CI: 1.09, 1.57] and those had higher physical activities (OR ¼ 1.36, 95% CI: 1.09, 1.57). Adolescents’ sex, obesity status and different cutoff points of FPG did not modify the association between the exposure to PM2.5 and FPG levels. Conclusion: Long-term exposure to PM2.5 was associated with increased FPG levels in Indonesian nondiabetic adolescents.Wenhua Yu, Dian Caturini Sulistyoningrum, Danijela Gasevic, Rongbin Xu, Madarina Julia, Indah Kartika Murni, Zhuying Chen, Peng Lu, Yuming Guo, Shanshan L

Effects of dietary sodium butyrate on growth performance, immune function, and intestinal microflora of Chinese soft-shelled turtle (Pelodiscus sinensis)

The Chinese soft-shelled turtle (Pelodiscus sinensis) has become increasingly susceptible to frequent diseases with the intensification of farming, which severely impacts the development of the aquaculture industry. Sodium butyrate (SB) is widely used as a feed additive due to its promotion of growth, enhancement of immune function, and antioxidative properties. This study aimed to investigate the effects of dietary SB on the growth performance, immune function, and intestinal microflora of Chinese soft-shelled turtles. A total of 300 Chinese soft-shelled turtles (mean weight: 11.36 ± 0.21g) were randomly divided into four groups with three parallel sets in each group. Each group was fed a diet supplemented with 0%, 0.005%, 0.01%, or 0.02% SB for 60 days. The results demonstrated an upward trend in weight gain rate (WGR) and specific growth rate (SGR) with increasing SB supplementation, and the experimental group fed with 0.02% SB showed a significant increase in WGR and SGR compared to other groups (P&lt; 0.05). These levels of SB also decreased the levels of feed conversion ratio (FCR) and the total cholesterol (TC) content of Chinese soft-shelled turtles, and the 0.02% SB was significantly lower than that of other groups (P&lt; 0.05). The activity of complement protein in vivo increased with increases in SB content, and the activities of complement C3 and C4 reached the highest level with 0.02% SB. The species abundance of the experimental group D fed with 0.02% SB was significantly higher than that of other groups (P&lt; 0.05). Furthermore, the relative abundance of Clostridium sensu stricto 1 was significantly increased with 0.02% SB (P&lt; 0.05). In conclusion, adding 0.02% SB to the diet improves the growth performance, feed digestion ability, and intestinal microbiota of Chinese soft-shelled turtles

Next steps for Human-Computer Integration

Human-Computer Integration (HInt) is an emerging paradigm in which computational and human systems are closely interwoven. Integrating computers with the human body is not new. However, we believe that with rapid technological advancements, increasing real-world deployments, and growing ethical and societal implications, it is critical to identify an agenda for future research. We present a set of challenges for HInt research, formulated over the course of a five-day workshop consisting of 29 experts who have designed, deployed, and studied HInt systems. This agenda aims to guide researchers in a structured way towards a more coordinated and conscientious future of human-computer integration

Repair of endonuclease- and transposon-induced DNA double strand breaks by host factors in Arabidopsis

The maize Ac/Ds transposable elements are members of the hAT transposon family. Ac/Ds transpose in diverse species and via a mechanism similar to that involved in V(D)J recombination. Here, we studied the repair of Ds transposition-induced hairpins by host factors in Arabidopsis. The results indicate that AtKu could position hairpin-opening to sites close to the hairpin apex. In the absence of AtMre11 or AtKu70, repair junctions have a higher frequency of small deletions (3-10bp) and larger deletions (11-50bp), respectively. Comparison of the repair of I-SceI- and Ds-induced DSB indicates that Ac transposase could influence Ds-induced DSB repair. In addition, analysis of I-SceI-induced DSB repair indicates that both AtKu70 and AtMre11 are critical for non-homologous end joining (NHEJ); AtKu70, but not AtMre11 affects its fidelity. In these respects, NHEJ in Arabidopsis resembles that in S. cerevisiae. However, in Arabidopsis, the repair junctions are error-prone, and microhomology-mediated end joining is not affected by AtKu70. Hence, its junction patterns are similar to those in mammals. These results indicate the dynamics of DSB repair among different organisms.</p

The transformation of images of Nezha and the changing cultural discourses in Chinese national style animated films from 1979 to 2019

Abstract Nezha is a mythological and dynamic character in the Chinese cultural discourse. In traditional China, the mythological character of Nezha represents a cultural integration between Daoism and Buddhism. By applying the Chinese animated films as an approach, this article focuses on exploring the changing cultural discourses of the Chinese national style in post-Maoist cinema through critical analysis of the character of Nezha and the deployment of Daoist philosophy in the construction of Chinese national style from the post-Cultural Revolution context to the post-Socialist context. This article examines two Chinese national-style animated films. The first film is Nezha Naohai (1979) which was produced in 1979, and another animated film is Nezha Zhi Motong Jiangshi (2019) which was produced in contemporary China. Through the film and cultural analysis of Nezha Naohai (1979), this article argues that the Daoist philosophy engages in the cultural discourse of Chinese national style and shapes the narrative, rhetoric, aesthetics and characterisation of Nezha in the post-Cultural Revolution context. The contemporary film Nezha Zhi Montong Jiangshi (2019) reflects an ideological change in the discourse of Chinese soft power states the power of Chinese culture including Daoist philosophy within a modern and scientific paradigm, which promotes a more international Chinese national identity and national style

Aphid Resistant Gene Transformation of Wheat by Biolistics

Wheat does not have a gene that can resist different aphid species on itself. I has been reported that potato proteinase inhibitor II is potentially effective protein for the control of these species. One major purpose of this research was to add a modified potato proteinase inhibitor II gene PI-IIK into wheat. Biolistics was employed to shoo he PI-IIK gene into wheat anther calli. The plants regenerated from these bombarded calli were screened for the transgene through PCR. 15 potentially transgenic fertile parts have been identified and further confirmed by semi-nested PCR. and Southen1 hybridization The transgene integration mechanisms were also studied. The results indicated that multiple copies of the transgene were integrated into the genomic DNA. The integration patterns include full length transgene(s) interspersed transgene(s), and rearranged transgenes Agrobacterium proteins might produce T-DNA-type insertion events in heat transformation through biolistics

A network meta-analysis of different acupuncture modalities in the treatment of bronchial asthma

Abstract Background Glucocorticoids and Beta-2 receptor agonists are commonly used for the treatment of asthma in clinical practice, while these agents are accompanied by adverse reactions of different kinds. Studies have shown that acupuncture is effective in treating bronchial asthma. However, different acupuncture modalities have different costs and skill requirements, and there remains a lack of comparisons between different acupuncture modalities. This study aims to assess the efficacy of various acupuncture modalities in the treatment of asthma. Methods The following databases were searched for randomized controlled trials (RCTs) on acupuncture for the treatment of bronchial asthma from database inception to August 26, 2022: PubMed, Embase, The Cochrane Library, Web of Science, Chinese Journal Full-text Database (CNKI), Wanfang Database (Wanfang Date), VIP Database (VIP), China Biology Medicine disc (CBM). Stata 15.1 software was used to conduct network meta-analysis. The risk of bias in the included studies was evaluated using the Cochrane Risk of Bias Assessment Tool 2 (RoB2). Results A total of 8,693 relevant studies were found, and 30 RCTs were included, involving 2,722 patients with bronchial asthma and eight acupuncture modalities: manual acupuncture, moxibustion, electroacupuncture, ignipuncture, flying needle acupuncture, acupoint catgut embedding, acupoint application, and warm-needle moxibustion. The other 29 studies had certain risks, with the quality graded as “moderate”. Among the pair-wise comparisons of statistical significance (p < 0.05), acupoint application was the most effective in improving pulmonary function (FEV1: Traditional medicine therapy-acupoint application [-7.29 (-12.11,-2.47)]; acupoint application-moxibustion [7.20 (0.28,14.11)]; FVC: acupoint application-Traditional medicine therapy [8.02 (2.54,13.50)]). Acupoint catgut embedding was the most effective in improving the ACT score of the patients (Traditional medicine therapy-acupoint catgut embedding [-4.29 (-7.94, -0.65)]; acupoint catgut embedding-moxibustion [5.52 (1.05,9.99)]). Conclusion Acupoint application has evident merits in improving the clinical response rate and pulmonary function, while acupoint catgut embedding can improve other secondary indicators. For the clinical treatment of asthma, acupoint application can be selected as a complementary and alternative therapy, while the other acupuncture therapies can also be considered according to the examination results of the patients