Predicted models of ITS1 rRNA secondary structure.

<p>Predicted models of A) <i>C</i>. <i>artocarpi</i>, B) <i>C</i>. <i>musae</i>, and C) <i>C</i>. <i>phyllostachysdis</i>. The structures contain three helices numbered H1–H3. The grey-colored parts of hairpin loops(T1, T2) are regions with variable numbers of nucleotides. The identity and difference of the structures among different <i>Cyphellophora</i> species are mapped on the 2D models.</p

<i>Cyphellophora musae</i>.

<p>A. SBFS signs on the surface of Japanese banana fruit. B. Colony on OA after 4 wk. C–G. Conidia and phialidic conidiogenous cells with collarettes. H. Intercalary conidiogenous cells with sessile collarettes. Bar = 5 μm.</p

<i>Cyphellophora phyllostachysdis</i>.

<p>A, colonies on bamboo with close-up view. B, Colony on OA after 4 wk. C–J, Phialidic conidiogenous cell and conidia. Microscopic morphology on PDA: C, D, E, G, H; OA: F, I, J. <i>Scale bars</i>: A = 1mm, B = 2mm, C–G = 5μm, H = 10μm, I, J = 5μm.</p

Key to species accepted in <i>Cyphellophora</i>.

<p>Key to species accepted in <i>Cyphellophora</i>.</p

Colonies of <i>Cyphellophora artocarpi</i> produced on jackfruit (original host) and on apple (pathogenicity test).

<p>A. On jackfruit. B. On apple.</p

Bayesian tree based on combined data set of ITS, LSU, TUB and RPB1 sequences.

<p>Bayesian posterior probability ≥ 0.5 and maximum parsimony bootstrap support (MP-BS) ≥ 50% are shown above and below the branches in first and second positions, respectively. New sequences generated in this study are printed in bold. The RPB1 sequences of <i>C</i>. <i>olivacea</i>, <i>C</i>. <i>sessilis</i> and <i>Cladophialophora immunda</i> are missing.</p

Three New Species of <i>Cyphellophora</i> (Chaetothyriales) Associated with Sooty Blotch and Flyspeck

<div><p>The genus <i>Cyphellophora</i> includes human- and plant-related species from mammal skin and nails, plant materials, and food. On the basis of analysis of ITS, LSU, TUB2 and RPB1 data and morphological characters, three new species, <i>Cyphellophora phyllostachysdis</i>, <i>C</i>. <i>artocarpi</i> and <i>C</i>. <i>musae</i>, associated with sooty blotch and flyspeck disease, were added to this genus. The 2D structure of ITS1 and ITS2 confirmed this taxonomic status. Pathogenicity tests on apple fruit indicated that <i>C</i>. <i>artocarpi</i> could be a sooty blotch and flyspeck pathogen of apple.</p></div

<i>Cyphellophora artocarpi</i>.

<p>A. SBFS signs occurring on twig of jackfruit. B, Colony on OA after 4 wk. C–G, Conidia and phialidic conidiogenous cells with collarettes. H–I, Conidiogenous cells. Bar = 5 μm.</p

ITS phylogeny of <i>Cyphellophora</i>.

<p>One of 100 equally parsimonious trees determined from maximum parsimony analysis of ITS sequences. The first number shown at each node is the Bayesian posterior probability (BPP ≥ 0.5) and the second number represents maximum parsimony bootstrap proportion (MP-BS ≥ 50%). The tree is rooted to <i>Cladophialophora immunda</i> isolate CBS 834.96; new sequences generated in this study are printed in bold.</p

Prediction of ITS2 rRNA secondary structure models of <i>C</i>. <i>artocarpi</i>, <i>C</i>. <i>musae</i>, and <i>C</i>. <i>phyllostachysdis</i>.

<p>Predicted models of A) <i>C</i>. <i>artocarpi</i>, B) <i>C</i>. <i>musae</i>, and C). <i>C</i>. <i>phyllostachysdis</i>. Five helices in the 2D structures are numbered H1–H5. Five hairpin loops (T1–T5) are highlighted with grey.</p