Fast Sodium-Ion Conduction in a Novel <i>Conjuncto</i>-Hydroborate of Na₄B₂₀H₁₈

Hydroborates have attracted extensive attention as a promising class of solid electrolytes for all-solid-state batteries. A conjuncto-hydroborate of Na4B20H18 is successfully developed and exhibits significantly promoted ionic conductivity and electrochemical stability compared to its monomer form of Na2B10H10. Moreover, the anion-mixing method is implemented and the mixed-anion electrolyte of Na4B20H18-3Na2B12H12 achieves an enhanced ionic conductivity exceeding 0.22 mS cm–1 at 25 °C, a remarkable Na-ion transference number up to 0.921 and an electrochemical stability window of 3.45 V vs Na+/Na at room temperature. Meanwhile, Na4B20H18-3Na2B12H12 also has remarkable stability against the Na electrode, and an all-solid-state battery with Na metal anode, TiS2 cathode, and Na4B20H18–3Na2B12H12 electrolyte delivers outstanding capacity retention of 92% after 50 cycles at 0.1C

Localization of TRPC6 in mouse hippocampus during the postnatal development.

<p>The immunohistochemical staining showed that TRPC6 was expressed in all regions of the hippocampus at P1 (B), P3 (C), P5 (D), P7 (E), P14 (F) and P21 (G). The negative control image was shown in (A). Scale bar, 200 µm. In suit hybridization of P1 (H) and P5 (I) brain sections showed the expression of TRPC6 in hippocampal neurons. Scale bar, 100 µm. The corresponding linear diagram of relative fluorescent intensity in glomeruli was shown in (J). Data were presented as means±S.D. n = 6/group. *<i>P</i><0.05, **<i>P</i><0.01 <i>vs.</i> adjacent age group.</p

Localization of TRPC6 in mouse renal cortex during postnatal development.

<p>Immunohistochemical staining of TRPC6 showed the expression of TRPC6 in the mouse renal cortex at P1 (B), P3 (C), P5 (D), P7 (E), P14 (F), P21 (G), P28 (H), and P49 (I). The negative control image was from the renal cortex, in which the primary antibody was a species-appropriate IgG (A). The corresponding linear diagram of relative fluorescent intensity in glomeruli was shown in (J). The sections from P1 to P5 showed that TRPC6 mostly expressed in comma-shaped body, S-shaped body and renal corpuscles of stage III (B-D). After P7 the expression was found in renal corpuscles and it was weakly positive (E-F). During the development, tubules were all observed. Scale bar, 100 µm. Data were presented as means±S.D. n = 6/group. *<i>P</i><0.05, **<i>P</i><0.01 <i>vs.</i> adjacent age group.</p

The immunoblot analysis of TRPC6 in mouse hippocampus during the development.

<p>Total lysates of tissues treated as indicated blotted with antibodies to TRPC6 at the indicated ages (A). Quantitative determination of TRPC6 expression showed an abrupt increase between P7 and P14 in mouse renal cortex during the development, and expression levels remained higher into adulthood (B). The expression of TRPC6 was normalized to β-actin expression. Data were presented as means±S.D. *<i>P</i><0.05, **<i>P</i><0.01 <i>vs.</i> adjacent age group.</p

The weight of maintenance arcs.

<p>The weight of maintenance arcs.</p

The relationship between the train graph and the train-set utilization plan.

<p>The relationship between the train graph and the train-set utilization plan.</p

Effect of 3-MA and rapamycin.

<p>Differentiated PC12 cells were incubated with culture medium, 10 μmol/L Aβ_25–35, 10 μmol/L 3-MA, 10ng/mL rapamycin, 10 μmol/L Aβ_25–35+10 μmol/L 3-MA, 10 μmol/L Aβ_25–35+10⁻¹⁰ mol/L triptolide, and 10 μmol/L Aβ_25–35+10⁻¹⁰ mol/L triptolide +10ng/mL rapamycin, for 24 hours. The cell viability was determined by MTT. Data represents the mean ± S.E.M. n = 6/group. *<i>P</i> < 0.05, **<i>P</i> < 0.01 vs. control group. ^#<i>P</i> < 0.05, ^##<i>P</i> < 0.01 vs. 10 μmol/L Aβ_25–35 group. ^&<i>P</i> < 0.05, ^&&<i>P</i> < 0.01 vs.10 μmol/L Aβ_25–35+10⁻¹⁰ mol/L triptolide group.</p

Four essential procedures.

<p>Four essential procedures.</p

Viability of differentiated PC12 cells treated with different concentrations of triptolide.

<p>Differentiated PC12 cells were incubated with different concentrations of triptolide (10⁻¹¹, 10⁻¹⁰, 10⁻⁹ mol/L) for 24 hours. Cell viability was determined by MTT assay. Data represents the mean ± S.E.M. n = 6/group.</p

Measurement of ROS generation.

<p>ROS levels were determined by flow cytometric analysis. The level of ROS generation was calculated as follows: the level of ROS (%) = the percentage of DCF-positive cell in M1 region. Differentiated PC12 cells were incubated with 10 μmol/L Aβ_25–35 (b), and 10 μmol/L Aβ_25–35+10⁻¹⁰ mol/L triptolide (c) for 24 hours. Cells were cultured with culture medium (a). The bar graph is shown in (d). Data represents the mean ± S.E.M. n = 3/group. **<i>P</i> < 0.01 vs. control group. ^##<i>P</i> < 0.01 vs. 10 μmol/L Aβ_25–35 group.</p