Extraction Process of Amino Acids with Deep Eutectic Solvents-Based Supported Liquid Membranes

The separation and purification process of amino acids (AAs) accounts for 80% of the total production cost. So the development of new technology for the separation and purification of AAs is very important. In this research, supported liquid membranes (SLMs) based on deep eutectic solvents (DESs) were successfully applied for the extraction of AAs. A series of DESs were designed, synthesized, and screened to obtain suitable ones as the supported phase. Some important factors, such as effect of types of DESs and membranes, pH and concentration of Trp were investigated. Higher extraction efficiency was achieved. ChCl/PTS was found to be the most efficient one. Results show that the initial concentration of Trp in the feed phase is 0.1 mM, and pH = 1.0 in the feed phase and pH = 13.0 in receiving phase is optimal for extraction, under which the extraction efficiency can reach 86.1%. ChCl/PTS/Asp (1/4/1) of SLM has the best stability, the weight loss of SLM is 0.38%. Finally, ¹H NMR shows that the hydrogen bonding interaction between DESs and Trp is probably the main driven force for this specific process. This research would provide a new process for the separation and purification of AAs with SILMs technique

Supplementary document for Channel transformer U-Net: an automatically and effective skeletons extraction network for electronic speckle pattern interferometry - 6186404.pdf

Supplementary Materials for CCT and experimental result

A new triterpenoid compound from stems and leaves of American ginseng

<div><p>A new dammarane-type triterpenoid compound was isolated from stems and leaves of American ginseng. The structure of the new sapogenin was elucidated by the combined analysis of NMR and HR-ESI-MS as dammar-20<i>S</i>, 25<i>S</i>-epoxy-3β, 12β, 26-triol (1). Compound <b>1</b> showed cytotoxic effect on human SM7721 and human Hela cells <i>in vitro</i>.</p></div

Crowned Ionic Liquids for Biomolecular Interaction Analysis

On the basis of affinity recognition with positively charged side chains in peptides and proteins, a series of crowned 1,2,3-triazolium ionic liquids (<b>CIL 1-6</b>) was developed and found to be capable of quantitatively extracting peptides and proteins from the aqueous layer into the ionic liquid phase. All of the synthesized <b>CIL 1-6</b> are liquid at room temperature. This is the first example of biomolecular recognition of both lysine- and arginine-containing peptides and proteins by CILs in pure ionic liquid phase

Customized Lanthanide Nanobiohybrids for Noninvasive Precise Phototheranostics of Pulmonary Biofilm Infection

A noninvasive strategy for in situ diagnosis and precise treatment of bacterial biofilm infections is highly anticipated but still a great challenge. Currently, no in vivo biofilm-targeted theranostic agent is available. Herein, we fabricated intelligent theranostic alginate lyase (Aly)-NaNdF4 nanohybrids with a 220 nm sunflower-like structure (NaNdF4@DMS-Aly) through an enrichment-encapsulating strategy, which exhibited excellent photothermal conversion efficiency and the second near-infrared (NIR-II) luminescence. Benefiting from the site-specific targeting and biofilm-responsive Aly release from NaNdF4@DMS-Aly, we not only enabled noninvasive diagnosis but also realized Aly-photothermal synergistic therapy and real-time evaluation of therapeutic effect in mice models with Pseudomonas aeruginosa biofilm-induced pulmonary infection. Furthermore, such nanobiohybrids with a sheddable siliceous shell are capable of delaying the NaNdF4 dissolution and biodegradation upon accomplishing the therapy, which is highly beneficial for the biosafety of theranostic agents

Table_1_Planting grass enhances relations between soil microbes and enzyme activities and restores soil functions in a degraded grassland.DOCX

IntroductionForage culture is a common way to restore degraded grasslands and soil functions, in which the reconstruction of the soil microbial community and its relationship with extracellular enzyme activity (EEAs) can characterize the recovery effects of degraded grasslands. However, the impacts of forage culture on the interaction between soil microbes and EEAs and whether the recovery effect of soil functions depends on the varying degradation statuses remain unclear.MethodsWe conducted a plantation of a dominant grass, Leymus chinensis, in the soil collected from severe, moderate, light, and non-degradation statuses in the Songnen grassland in northeastern China. We measured soil microbial diversity and soil EEAs, and predicted microbial functional groups using FUNGuild.ResultsThe results showed that L. chinensis culture promoted soil bacterial alpha diversity and soil EEAs only in the moderate degradation status, indicating a dramatic dependence of the recovery effects of the grass culture on degradation status of the grassland. After planting L. chinensis for 10 weeks, a decreasing trend in the chemoheterotrophy and nitrate-reduction microbial functional groups was found. In contrast, the abundance of the nitrogen (N)-fixing microbial functional group tended to increase. The positive correlation between soil EEAs and the nitrate-reduction and N-fixing microbial functional groups was enhanced by planting L. chinensis, indicating that grass culture could promote soil N cycle functions.ConclusionWe illuminate that grass culture may promote the restoration of soil functions, especially soil N cycling in degraded grasslands, and the recovery effect may depend on the grassland degradation status. We emphasized that selection of the plant species for restoration of grasslands needs to consider the restoration effects of microbial functional groups and soil functions.</p

Flavonoid biosynthesis in <i>Camellia oleifera</i>.

<p>Flavonoid biosynthesis in <i>Camellia oleifera</i>.</p

Distribution of gene ontology (GO) function in <i>Camellia oleifera</i>.

<p>The y-axis indicates the number of unigenes.</p

Transcription factors (TFs) identified in <i>Camellia oleifera</i>.

<p>Transcription factors (TFs) identified in <i>Camellia oleifera</i>.</p

Physiological changes in the leaves of two <i>Camellia oleifera</i> cultivars in response conditions of drought.

<p>(A) Relative water content; (B) Relative conductivity; (C) Chlorophyll content; (D) Peroxidase activity; (E) MDA content; and (F) Soluble sugar content, were investigated under 20% PEG at four time points (0, 12, 24, and 36 h).</p