The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma

Protein kinase C epsilon (PKCε), an oncogene overexpressed in several human cancers, is involved in cell proliferation, migration, invasion, and survival. However, its roles in clear cell renal cell carcinoma (RCC) are unclear. This study aimed to investigate the functions of PKCε in RCC, especially in clear cell RCC, to determine the possibility of using it as a therapeutic target. By immunohistochemistry, we found that the expression of PKCε was up-regulated in RCCs and was associated with tumor Fuhrman grade and T stage in clear cell RCCs. Clone formation, wound healing, and Borden assays showed that down-regulating PKCε by RNA interference resulted in inhibition of the growth, migration, and invasion of clear cell RCC cell line 769P and, more importantly, sensitized cells to chemotherapeutic drugs as indicated by enhanced activity of caspase-3 in PKCε siRNA-transfected cells. These results indicate that the overexpression of PKCε is associated with an aggressive phenotype of clear cell RCC and may be a potential therapeutic target for this disease

Challenges and perspectives in continuous glucose monitoring

Diabetes is a global epidemic that threatens the health and well-being of hundreds of millions of people. The first step in patient treatment is to monitor glucose levels. Currently this is most commonly done using enzymatic strips. This approach suffers from several limitations, namely it requires a blood sample and is therefore invasive, the quality and the stability of the enzymatic strips vary widely, and the patient is burdened by performing the measurement themselves. This results in dangerous fluctuations in glucose levels often going undetected. There is currently intense research towards new approaches in glucose detection that would enable non-invasive continuous glucose monitoring (CGM). In this review, we explore the state-of-the-art in glucose detection technologies. In particular, we focus on the physical mechanisms behind different approaches, and how these influence and determine the accuracy and reliability of glucose detection. We begin by reviewing the basic physical and chemical properties of the glucose molecule. Although these play a central role in detection, especially the anomeric ratio, they are surprisingly often overlooked in the literature. We then review state-of-the art and emerging detection methods. Finally, we survey the current market for glucometers. Recent results show that past challenges in glucose detection are now being overcome, thereby enabling the development of smart wearable devices for non-invasive continuous glucose monitoring. These new directions in glucose detection have enormous potential to improve the quality of life of millions of diabetics, as well as offer insight into the development, treatment and even prevention of the disease

Numerical Simulation of Fluid Transport along Parallel Vanes for Vane Type Propellant Tanks

Fuel tanks are a core component in satellites that manage the propellant. This study numerically analyzed the fluid transport with parallel guide vanes in a vane type surface tension tank. Flow3D was used to simulate fluid transport in microgravity in a scale model with 15% liquid filling rate with comparisons to experimental data. Then, the simulations were used to compare the leading edge climbing speed for various working conditions and the liquid volume below a specified cross section for various liquid filling rates, various numbers of vanes. The results show that the liquid climbing process on the guide plate in the plate tank can be divided into the liquid level repositioning stage after the gravitational force is suddenly removed and the stable fluid transport stage. Throughout the entire capillary flow stage, the liquid leading edge climbing rate is not related to the filling rate of the number of vanes and the fluid transport efficiency of a single guide vane is independent of the total number of vanes

Quantitative Analysis of the Blending Degree of Virgin and RAP Binders in Recycled Asphalt Mixtures with a High RAP Content

Recycled asphalt mixtures (RAM), which are prepared by blending reclaimed asphalt pavement (RAP), virgin bitumen and mineral additives, provide a variety of advantages, including resource recycling, reductions in costs, and reduced negative environmental impacts. However, multiple agencies have expressed concerns about the utilization ratio of RAP; thus, a comprehensive understanding of the blending degree of virgin and RAP binders in RAM would be significantly helpful for promoting the application of RAP. This study aims to quantitatively analyze the blending degree of virgin and RAP binders in RAM with high RAP contents. Carboxyl-terminated butadiene acrylonitrile (CTBN) was utilized as a tracer to mark the virgin bitumen; in addition, Fourier transform infrared (FTIR) spectroscopy was used to develop the structural index of CTBN (ICTBN). By establishing the standard curve between ICTBN and the CTBN content, the blending degree of virgin and RAP binders at different locations within RAM can be determined quantitatively. The study results indicate that the RAP binder was completely blended with the virgin bitumen in the outer RAP layer. However, the blending degree decreased with an increase in the RAP depth, and the blending degree in the inner RAP layer was only approximately half that which was found in the case of complete blending

Insight into the Storage Mechanism of Sandwich-Like Molybdenum Disulphide/Carbon Nanofibers Composite in Aluminum-Ion Batteries

Aluminum-ion batteries (AIBs) have become a research hotspot in the field of energy storage due to their high energy density, safety, environmental friendliness, and low cost. However, the actual capacity of AIBs is much lower than the theoretical specific capacity, and their cycling stability is poor. The exploration of energy storage mechanisms may help in the design of stable electrode materials, thereby contributing to improving performance. In this work, molybdenum disulfide (MoS2) was selected as the host material for AIBs, and carbon nanofibers (CNFs) were used as the substrate to prepare a molybdenum disulfide/carbon nanofibers (MoS2/CNFs) electrode, exhibiting a residual reversible capacity of 53 mAh g−1 at 100 mA g−1 after 260 cycles. The energy storage mechanism was understood through a combination of electrochemical characterization and first-principles calculations. The purpose of this study is to investigate the diffusion behavior of ions in different channels in the host material and its potential energy storage mechanism. The computational analysis and experimental results indicate that the electrochemical behavior of the battery is determined by the ion transport mechanism between MoS2 layers. The insertion of ions leads to lattice distortion in the host material, significantly impacting its initial stability. CNFs, serving as a support material, not only reduce the agglomeration of MoS2 grown on its surface, but also effectively alleviate the volume expansion caused by the host material during charging and discharging cycles