Streptococcus suis Sequence Type 7 Outbreak, Sichuan, China

An outbreak of Streptococcus suis serotype 2 emerged in the summer of 2005 in Sichuan Province, and sporadic infections occurred in 4 additional provinces of China. In total, 99 S. suis strains were isolated and analyzed in this study: 88 isolates from human patients and 11 from diseased pigs. We defined 98 of 99 isolates as pulse type I by using pulsed-field gel electrophoresis analysis of SmaI-digested chromosomal DNA. Furthermore, multilocus sequence typing classified 97 of 98 members of the pulse type I in the same sequence type (ST), ST-7. Isolates of ST-7 were more toxic to peripheral blood mononuclear cells than ST-1 strains. S. suis ST-7, the causative agent, was a single-locus variant of ST-1 with increased virulence. These findings strongly suggest that ST-7 is an emerging, highly virulent S. suis clone that caused the largest S. suis outbreak ever described

Histomorphological study of the spinal growth plates from the convex side and the concave side in adolescent idiopathic scoliosis

Asymmetrical growth of the vertebrae has been implicated as one possible etiologic factor in the pathogenesis of adolescent idiopathic scoliosis. The longitudinal vertebral growth derives from the endochondral ossification of the vertebral growth plate. In the present study, the growth plates from the convex and concave side of the vertebrae were characterized by the method of histology and immunohistochemistry to evaluate the growth activity, cell proliferation, and apoptosis. Normal zoned architectures were observed in the convex side of the growth plate and disorganized architectures in the concave side. The histological grades were significantly different between the convex and the concave side of the growth plate in the apex vertebrae (P < 0.05). The histological difference was also found significant statistically between end vertebrae and apex vertebrae in the concave side of vertebral growth plates (P < 0.05). The proliferative potential indexes and apoptosis indexes of chondrocytes in the proliferative and hypertrophic zone in the convex side were significantly higher than that in the concave side in the apex vertebral growth plate (P < 0.05). There was a significant difference of the proliferative potential index (proliferating cell nuclear antigen, PCNA index) between convex side and concave side at the upper end vertebra (P < 0.05). The difference of the proliferative potential index and apoptosis index were found significant statistically in the concave side of the vertebral growth plate between end vertebrae and apex vertebrae (P < 0.05). The same result was also found for the apoptosis index (terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate biotin nick end labeling assay, TUNEL index) in the convex side of vertebral growth plate between end vertebrae and apex vertebrae (P < 0.05). Some correlation were found between radiographic measurements and proliferation and apoptosis indexes. The difference in histological grades and cellular activity between the convex and concave side indicated that the bilateral growth plate of the vertebrae in AIS patients have different growth kinetics which may affect the curve progression

A simulation test of the impact on soil moisture by agricultural machinery

To study the impact by agricultural machinery on changes in soil moisture, we used a simulated test method employing round iron plate based on the ground pressure ratio between the front and rear wheels of wheeled tractors and crawler tractors. We conducted soil compactions with five pressure loads (35, 98, 118, 196 and 345 kg), and measured soil moistures at different depths and under different compaction times, as well as compared those before the loads was applied. The results indicate that soil moisture was generally lost after compaction by agricultural machinery and its loss was related to pressure load, soil depth and compaction times. Generally, moisture loss increased with the increase of pressure load and mostly occurred at the soil surface (0 to 5 cm) for light loads (&lt;110 kg), but at deeper soil for heavy loads (&gt;110 kg). Moreover, the moisture loss decreased gradually with the increases in soil depth for light loads (37 and 98 kg), although it was first increased and then quickly decreased for heavy loads (&gt;= 118 kg). The loss of soil moisture by 5 compactions was in similar pattern with 1 compaction, but was much larger with the gap by 0.5 to 1.5% between them

The Study of Sewage Sludge Thermo-Drying Efficiency

AbstractWith the population exploding and improvement of urbanization, the sewage sludge production increases rapidly with the increasing of sewage. However, high water content has been a big problem for sewage sludge treatment and disposal. Thermo-heating is one of the mature methods to reduce the water content in sewage sludge, but the promotion of thermo drying is very restricted by its high cost because of abundant consumption of energy and its expensive equipments. In order to save energy and reduce investment for equipments, we studied the low-temperature thermo-drying efficiency by varying the temperature from 100°C to 200°C, and changing the shape of the sewage sludge sample, We tried three shapes (cake, cylindrical and spherical) and three temperature (at 150°C,175°C and 200°C). It is found that cake sludge has the highest drying efficiency and spherical sludge is the lowest in each condition. At 200°C the drying efficiency could get the highest point

Effects of litter and root manipulations on soil carbon and nitrogen in a Schrenk's spruce (Picea schrenkiana) forest.

Plant detritus represents the major source of soil carbon (C) and nitrogen (N), and changes in its quantity can influence below-ground biogeochemical processes in forests. However, we lack a mechanistic understanding of how above- and belowground detrital inputs affect soil C and N in mountain forests in an arid land. Here, we explored the effects of litter and root manipulations (control (CK), doubled litter input (DL), removal of litter (NL), root exclusion (NR), and a combination of litter removal and root exclusion (NI)) on soil C and N concentrations, enzyme activity and microbial biomass during a 2-year field experiment. We found that DL had no significant effect on soil total organic carbon (SOC) and total nitrogen (TN) but significantly increased soil dissolved organic carbon (DOC), microbial biomass C, N and inorganic N as well as soil cellulase, phosphatase and peroxidase activities. Conversely, NL and NR reduced soil C and N concentrations and enzyme activities. We also found an increase in the biomass of soil bacteria, fungi and actinomycetes in the DL treatment, while NL reduced the biomass of gram-positive bacteria, gram-negative bacteria and fungi by 5.15%, 17.50% and 14.17%, respectively. The NR decreased the biomass of these three taxonomic groups by 8.97%, 22.11% and 21.36%, respectively. Correlation analysis showed that soil biotic factors (enzyme activity and microbial biomass) and abiotic factors (soil moisture content) significantly controlled the change in soil C and N concentrations (P < 0.01). In brief, we found that the short-term input of plant detritus could markedly affect the concentrations and biological characteristics of the C and N fractions in soil. The removal experiment indicated that the contribution of roots to soil nutrients is greater than that of the litter

Built-up edge formation mechanisms in orthogonal cutting of wood-plastic composite

This project aims to improve the machinability of wood-plastic composites by understanding chip and built-up edge formation, so as to help manufacturers optimize cutting performance and product quality. Chip formation and built-up edge were studied during orthogonal cutting of wood polyethylene composite with cemented carbide cutters under different conditions. During the orthogonal cutting process, segmental, ribbon, and element chips were generated. The cutting depth was found to have a great impact on the types of chips that formed. Additionally, a built-up edge was found during wood-plastic composite machining, with debris only attaching to the tool's rake face due to thermo-mechanical coupling. Such built-up edges hinder cutting stability and surface quality. Furthermore, variations in the accumulation of debris on the built-up edge corresponded to changes in cutting force and temperature. In fact, both cutting force and temperature proved to be inversely related to the rake angle and positively correlated to the cutting speed and depth. Therefore, to achieve better cutting stability and surface quality for wood-plastic composites, a larger rake angle and a reduced cutting depth are recommended because they reduce the accumulation of debris and the formation of built-up edge.Validerad;2022;Nivå 2;2022-11-29 (joosat);Funder: National Natural Science Foundation of China (31971594); Natural Science Foundation of the Jiangsu Higher Education Institutions of China (21KJB220009); Self-Made Experimental and Teaching Instruments of Nanjing Forestry University (nlzzyq202101); Technology Innovation Alliance of Wood/Bamboo Industry (TIAWBI2021-08); International Cooperation Joint Laboratory for Production, Education, Research and Application of Ecological Health Care on Home Furnishing</p

Research on Thermal Error Modeling of Motorized Spindle Based on BP Neural Network Optimized by Beetle Antennae Search Algorithm

High-speed motorized spindle heating will produce thermal error, which is an important factor affecting the machining accuracy of machine tools. The thermal error model of high-speed motorized spindles can compensate for thermal error and improve machining accuracy effectively. In order to confirm the high precision thermal error model, Beetle antennae search algorithm (BAS) is proposed to optimize the thermal error prediction model of motorized spindle based on BP neural network. Through the thermal characteristic experiment, the A02 motorized spindle is used as the research object to obtain the temperature and axial thermal drift data of the motorized spindle at different speeds. Using fuzzy clustering and grey relational analysis to screen temperature-sensitive points. Beetle antennae search algorithm (BAS) is used to optimize the weights and thresholds of the BP neural network. Finally, the BAS-BP thermal error prediction model is established. Compared with BP and GA-BP models, the results show that BAS-BP has higher prediction accuracy than BP and GA-BP models at different speeds. Therefore, the BAS-BP model is suitable for prediction and compensation of spindle thermal error

Machinability of stone-plastic materials during diamond planing

This paper investigated the machinability of a stone–plastic composite (SPC) via orthogonal cutting with diamond cutters. The objective was to determine the effect of cutting depth on its machinability, including cutting forces, heat, chip formation, and cutting quality. Increased cutting depth promoted an increase in both frictional and normal forces, and also had a strong influence on the change in normal force. The cutting temperatures of chips and tool edges showed an increasing trend as cutting depth increased. However, the cutting heat was primarily absorbed by chips, with the balance accumulating in the cutting edge. During chip formation, the highest von Mises strain was mainly found in SPC ahead of the cutting edge, and the SPC to be removed partially passed its elastic limit, eventually forming chips with different shapes. Furthermore, the average surface roughness and the mean peak-to-valley height of machined surfaces all positively correlated to an increase in cutting depth. Finally, with an increase in cutting depth, the chip shape changed from tubular, to ribbon, to arc, to segmental, and finally, to helical chips. This evolution in chip shape reduced the fluctuation in cutting force, improving cutting stability and cutting quality.Validerad;2019;Nivå 1;2019-04-09 (inah)</p