Data presented in the paper 'Effects of sediment disturbance regimes on Spartina seedling establishment: implications for salt marsh creation and restoration'

The dataset contains seedling survival, critical erosion depth and morphological traits for Spartina alterniflora and Spartina anglica when been exposed to a set of accretion/erosion regimes in the laboratory. The study aims at gaining quantitative insight into how salt marsh seedling survival is affected by short-term sediment dynamics, and to what extent this may be mitigated by morphological adjustments by the plant

Vegetation recovery on neighboring tidal flats forms an Achilles’ heel of tidal marsh resilience to sea level rise

Supplementary data for the paper: "Revegetation of tidal flats forms an Achilles’ heel of saltmarsh resilience to sea level rise". This dataset includes: 1) a csv file that contains field data of seed persistence and corresponding wave conditions at each site; 2) R codes (two versions) for the marsh revegetation model

Data presented in the paper "Quantifying bed level change at the transition of tidal flat and salt marsh: can we understand the lateral location of the marsh edge?"

Bed level dynamics at the interface of the salt marsh and tidal flat have been highlighted as a key factor connecting the long-term bio-geomorphological development of the marsh to large-scale physical forcing. Hence, we aim to obtain insight into the factors confining the location of the marsh edge (i.e., boundary between tidal flat and salt marsh). A unique dataset was collected, containing measurements of daily bed level changes (i.e., integrative result of physical forcing and sediment properties) at six intertidal transects in the North Sea area. Moreover, various biophysical parameters were measured, such as sediment characteristics, waves, inundation time and chlorophyll-a levels

High resolution bed level change and synchronized biophysical data from 10 tidal flats in northwestern Europe

Tidal flats provide valuable ecosystem services such as flood protection and carbon sequestration. Erosion and accretion processes govern the eco-geomorphic evolution of intertidal ecosystems (marshes and bare flats), and hence substantially affect their valuable ecosystem services. To understand the intertidal ecosystem development, high-frequency bed-level change data are thus needed. However, such datasets are scarce due to the lack of suitable methods that do not involve excessive labour and/or instrument cost. By applying newly-developed Surface Elevation Dynamics sensors (SED-sensors), we obtained unique high-resolution daily bed-level change data sets in the period 2013-2017 from 10 saltmarsh sites situated in the Netherlands, Belgium and Britain in contrasting physical and biological settings. At each site, multiple sensors were deployed for 9-20 months to ensure sufficient spatial and temporal coverage of highly variable bed level change processes. The bed level change data are provided with synchronized hydrodynamic data, i.e. water level, wave height, tidal current velocity, and medium grain size (D50) as well as (for some sites) chlorophyll-a level and organic matter content of the surface sediment. This dataset has revealed diverse spatial morphodynamic patterns over daily to seasonal scales, which are valuable to theoretical and model development. On the daily scale, this dataset is particularly instructive as it includes a number of storm events, the response to which can be detected in the bed level change observations. Such data are rare but useful to study tidal flat response to highly energetic conditions

CRISPR–Cas9-mediated genome editing in apple and grapevine

The CRISPR–Cas9 genome-editing tool and the availability of whole-genome sequences from plant species have revolutionized our ability to introduce targeted mutations into important crop plants, both to explore genetic changes and to introduce new functionalities. Here, we describe protocols adapting the CRISPR–Cas9 system to apple and grapevine plants, using both plasmid-mediated genome editing and the direct delivery of CRISPR–Cas9 ribonucleoproteins (RNPs) to achieve efficient DNA-free targeted mutations in apple and grapevine protoplasts. We provide a stepwise protocol for the design and transfer of CRISPR–Cas9 components to apple and grapevine protoplasts, followed by verification of highly efficient targeted mutagenesis, and regeneration of plants following the plasmid-mediated delivery of components. Our plasmid-mediated procedure and the direct delivery of CRISPR–Cas9 RNPs can both be utilized to modulate traits of interest with high accuracy and efficiency in apple and grapevine, and could be extended to other crop species. The complete protocol employing the direct delivery of CRISPR–Cas9 RNPs takes as little as 2–3 weeks, whereas the plasmid-mediated procedure takes >3 months to regenerate plants and study the mutation