Dendrobium officinale

Background. Dendrobium officinale (DO) Kimura et Migo is a precious Chinese herb that is considered beneficial for health due to its antioxidant and antidiabetes properties, and so on. In this research, we try to determine the preventive effect of DO on the early complications of STZ-induced diabetic rats. Methods. Type 1 diabetic rats were produced with a single intraperitoneal injection of STZ (50 mg/kg). DO (1 g/kg/day) was then orally administered for 5 weeks. Blood glucose, TC, TG, BUN, CREA, and GSH-PX levels were determined, and electroretinographic activity and hypoalgesia were investigated. Pathological sections of the eyes, hearts, aortas, kidneys, and livers were analyzed. Results. Treatment with DO significantly attenuated the serum levels of TC, TG, BUN, and CREA, markedly increased the amplitudes of ERG a- and b-waves and Ops, and reduced the hypoalgesia and histopathological changes of vital organs induced by hyperglycemia. The protective effect of DO in diabetic rats may be associated with its antioxidant activity, as evidenced by the marked increase in the serum level of glutathione peroxidase. However, DO had no significant effect on blood glucose levels and bodyweight of diabetic rats. Conclusions. DO supplementation is an effective treatment to prevent STZ-induced diabetic complications

Methylation status of DDIT3 gene in Chronic Myeloid Leukemia

<p>Abstract</p> <p>Background</p> <p>DNA-damage-inducible transcript 3 (<it>DDIT3</it>), a candidate tumor suppressor gene (TSG), has been found involved in the regulation of cellular growth and differentiation. The epigenetic changes of TSGs are recently recognized as an abnormal mechanism contributing to the development of chronic myeloid leukemia (CML). The aim of this study was to investigate the methylation status of <it>DDIT3 </it>gene in CML patients.</p> <p>Methods</p> <p>The methylation status of <it>DDIT3 </it>promoter was detected in the bone marrow mononuclear cells from 53 patients with CML using methylation-specific PCR (MSP). The expression levels of <it>DDIT3 </it>and <it>bcr/abl </it>transcript were determined by real-time quantitative PCR (RQ-PCR). Clinical data of these patients were collected and analyzed.</p> <p>Results</p> <p>The aberrant methylation of <it>DDIT3 </it>gene promoter was found in 35 of 53 (66%) CML cases. Correlation was not found between <it>DDIT3 </it>promoter hypermethylation and the age, sex, hemoglobin concentration, platelet counts, chromosomal abnormalities, <it>bcr/abl </it>transcript, and staging of CML patients (<it>P </it>> 0.05), but found between <it>DDIT3 </it>promoter hypermethylation and WBC counts of CML cases (R = 0.781, <it>P </it>< 0.001). The level of <it>DDIT3 </it>transcript in CML patients was significantly lower than that in controls (median 3.28 vs 19.69, <it>P </it>< 0.001), however, there was no difference in the level of <it>DDIT3 </it>transcript between methylation-positive CML cases (0.05-65.32, median 2.13) and methylation- negative CML cases (0.12-126.04, median 3.92) (<it>P </it>> 0.05).</p> <p>Conclusion</p> <p>Our results demonstrate that aberrant methylation of <it>DDIT3 </it>occurs in CML frequently.</p

(E)-3-Bromo-N′-(5-bromo-2-hydroxy­benzyl­idene)benzohydrazide

The title compound, C14H10Br2N2O2, was synthesized by the reaction of 5-bromo­salicylaldehyde with an equimolar quantity of 3-bromo­benzohydrazide in methanol. The dihedral angle between the two benzene rings is 10.5 (4)°. In the crystal structure, mol­ecules are linked through inter­molecular N—H⋯O hydrogen bonds to form chains parallel to the c axis, and an intra­molecular O—H⋯N inter­action also occurs

Mechanical performance of 22SiMn2TiB steel welded with low-transformation-temperature filler wire and stainless steel filler wire

TX-80 low-transformation-temperature (LTT) welding wire was used to replace the traditional ER 307Si welding wire to realize the connection of 22SiMn2TiB armor steel in manual overlay welding. The previously existing issues, such as welding cracks, large welding deformation, and severe welding residual stress, were solved to ensure good strength and ductility requirements. In particular, with the same welding conditions, TX-80 LTT wire eliminates welding cracks. It reduces the welding deformation no matter the base pretreatment of pre-setting angle or no pre-setting angle. By comparison, it was found that the microstructure at the TX-80 weld is mainly composed of martensite and a small amount of retained austenite. In contrast, the microstructure of the ER 307Si weld consists of a large amount of austenite and a small amount of skeleton-like ferrite. The variation trend of residual stress and microhardness from the weld to the base were investigated and compared with the mechanical properties of base materials. The TX-80 and the ER 307Si tensile samples elongation is 6.76% and 6.01%, while the ultimate tensile strengths are 877 and 667 MPa, respectively. The average impact toughness at room temperature of the ER 307Si weld is 143.9 J/cm2, much higher than that of the TX-80 weld, which is only 36.7 J/cm2. The relationship between impact and tensile properties with microstructure species and distribution was established. In addition, the fracture surface of the tensile and the impact samples was observed and analyzed. Deeper dimples, fewer pores, larger radiation zone, and shear lips of TX-80 samples indicate better tensile ductility and worse impact toughness than those of ER 307Si weld.</p

Comparison of single cell sequencing data between two whole genome amplification methods on two sequencing platforms

Abstract Research based on a strategy of single-cell low-coverage whole genome sequencing (SLWGS) has enabled better reproducibility and accuracy for detection of copy number variations (CNVs). The whole genome amplification (WGA) method and sequencing platform are critical factors for successful SLWGS (<0.1 × coverage). In this study, we compared single cell and multiple cells sequencing data produced by the HiSeq2000 and Ion Proton platforms using two WGA kits and then comprehensively evaluated the GC-bias, reproducibility, uniformity and CNV detection among different experimental combinations. Our analysis demonstrated that the PicoPLEX WGA Kit resulted in higher reproducibility, lower sequencing error frequency but more GC-bias than the GenomePlex Single Cell WGA Kit (WGA4 kit) independent of the cell number on the HiSeq2000 platform. While on the Ion Proton platform, the WGA4 kit (both single cell and multiple cells) had higher uniformity and less GC-bias but lower reproducibility than those of the PicoPLEX WGA Kit. Moreover, on these two sequencing platforms, depending on cell number, the performance of the two WGA kits was different for both sensitivity and specificity on CNV detection. The results can help researchers who plan to use SLWGS on single or multiple cells to select appropriate experimental conditions for their applications

Strange Quasar Candidates with Abnormal Astrometric Characteristics from Gaia EDR3 and SDSS (SQUAB-II): Optical Identifications

There are some strange quasars with multiple Gaia detections or observed with abnormal astrometric characteristics, such as with large proper motions or significant astrometric noises. Those strange quasars could be potential candidates of quasar-star pairs, dual quasars (DQs), or lensed quasars (LQs). Searching for both DQs and LQs is of great importance in many fields of astrophysics. Here in this work, we select 143 SDSS spectroscopically confirmed quasars that have multiple Gaia EDR3 detections within 1 arcsec of the SDSS quasar' position. We apply several optical identification methods to classify this sample. We firstly exclude 65 quasar-star pairs via their stellar features including their parallaxes and proper motions, stellar features in the SDSS spectra, or via the colour-colour diagram. Based on the spectral-fitting results, we find 2 DQ candidates, one of which presents a double-peaked [O III] emission line feature and the other shows a broad $H_{\beta}$ velocity offset ($\sim$ 870 $km s^{-1}$) relative to the [O III] $\lambda$5007 line. Via the colour difference method, we further find 56 LQ candidates with similar colours in their multiple images. We also cross-match 143 objects with the HST archive and find 19 targets with archival HST images. Our classification results of those 19 targets are mainly consistent with previous works.Comment: 18 pages, 11figures, accepted for publication in MNRA

Changes in Volatile Profiles and Activity of Hydroperoxide Lyase and Alcohol Dehydrogenase During the Development of Cabernet Sauvignon Grapes (Vitis vinifera L.)

In this study we focused on the development of Cabernet Sauvignon grapes and investigated changes in theactivity of alcohol dehydrogenase (ADH) and hydroperoxide lyase (HPL) in different tissues. We sampledgrape skin at four, six, seven, eight, nine, 10, 12, 14 and 16 weeks after anthesis; developing flowers whenblooming at 0%, 5%, 50%, and 90%; and leaves at two and four weeks before anthesis and at two, four,six, eight, nine, and 10 weeks after anthesis. We also examined the type and fluctuation of volatile contents.ADH activity increased with the development of flowers and grape skins, which led to the increasing oftypes and concentration of alcohols. Low levels of 9-HPL led to low concentrations of C9 compounds.According to this paper, C6 compounds became abundant with the development of grape berries, while theactivity of 13-HPL kept at a low level in the flowers and grape skins. There might have been a high level of13-HPL activity from the end of flowering until fruit setting that we did not detect. Furthermore, similarC6 and C5 compounds were detected across all tissues, including hexanal, (E)-2-hexenal, (Z)-3-hexenal,(Z)-2-penten-1-ol, (Z)-3-hexen-1-ol, 1-hexanol and 3-hexen-1-ol. Generally speaking, the concentrations ofC6 and C5 compounds could be used as the criterion of maturation of the three grape tissues

On the Origin of the Strong Optical Variability of Emission-line Galaxies

Emission-line galaxies (ELGs) are crucial in understanding the formation and evolution of galaxies, while little is known about their variability. Here we report the study on the optical variability of a sample of ELGs selected in the COSMOS field, which has narrow-band observations in two epochs separated by $\gtrsim$ 12 years. This sample was observed with Suprime-Cam (SC) and Hyper Suprime-Cam (HSC) on the $Subaru$ telescope in NB816 and $i'/i$ bands, respectively. After carefully removing the wing effect of a narrow-band filter, we check the optical variability in a sample of 181 spectroscopically confirmed ELGs. We find that 0 (0/68) Ha emitters, 11.9% (5/42) [OIII] emitters, and 0 (0/71) [OII] emitters show significant variability ($|\Delta m_{NB}| \geq 3\,\sigma_{\Delta m_{NB,AGN}} = 0.20\, mag$) in the two-epoch narrow-band observations. We investigate the presence of active galactic nucleus (AGN) in this variable ELG (var-ELG) sample with three methods, including X-ray luminosity, mid-infrared activity, and radio-excess. We find zero bright AGN in this var-ELG sample, but cannot rule out the contribution from faint AGN. We find that SNe could also dominate the variability of the var-ELG sample. The merger morphology shown in the HST/F814W images of all the var-ELG sample is in agreement with the enhancement of star formation, i.e., the SNe activity.Comment: 20 pages, 10 figures, accepted for publication in The Astrophysical Journa