70,887 research outputs found
Interdecadal variability of winter precipitation in Southeast China
Interdecadal variability of observed winter precipitation in Southeast China (1961–2010) is characterized by the first empirical orthogonal function of the three-monthly Standardized Precipitation Index (SPI) subjected to a 9-year running mean. For interdecadal time scales the dominating spatial modes represent monopole features involving the Arctic Oscillation (AO) and the sea surface temperature (SST) anomalies. Dynamic composite analysis (based on NCEP/NCAR reanalyzes) reveals the following results: (1) Interdecadal SPI-variations show a trend from a dryer state in the 1970s via an increase during the 1980s towards stabilization on wetter conditions commencing with the 1990s. (2) Increasing wetness in Southeast China is attributed to an abnormal anticyclone over south Japan, with northward transport of warm and humid air from the tropical Pacific to South China. (3) In mid-to-high latitudes the weakened southward flow of polar airmasses induces low-level warming over Eurasia due to stronger AO by warmer zonal temperature advection. This indicates that AO is attributed to the Southeast China precipitation increase influenced by circulation anomalies over the mid-to-high latitudes. (4) The abnormal moisture transport along the southwestern boundary of the abnormal anticyclone over south Japan is related to anomalous south-easterlies modulated by the SST anomalies over Western Pacific Ocean; a positive (negative) SST anomaly will strengthen (weaken) warm and humid air transport, leading to abundant (reduced) precipitation in Southeast China. That is both AO and SST anomalies determine the nonlinear trend observed in winter precipitation over Southeast China
Calculating Biological Behaviors of Epigenetic States in Phage lambda Life Cycle
Gene regulatory network of lambda phage is one the best studied model systems
in molecular biology. More 50 years of experimental study has provided a
tremendous amount of data at all levels: physics, chemistry, DNA, protein, and
function. However, its stability and robustness for both wild type and mutants
has been a notorious theoretical/mathematical problem. In this paper we report
our successful calculation on the properties of this gene regulatory network.
We believe it is of its first kind. Our success is of course built upon
numerous previous theoretical attempts, but following 3 features make our
modeling uniqu:
1) A new modeling method particular suitable for stability and robustness
study;
2) Paying a close attention to the well-known difference of in vivo and in
vitro;
3) Allowing more important role for noise and stochastic effect to play.
The last two points have been discussed by two of us (Ao and Yin,
cond-mat/0307747), which we believe would be enough to make some of previous
theoretical attempts successful, too. We hope the present work would stimulate
a further interest in the emerging field of gene regulatory network.Comment: 16 pages, 3 figures, 1 tabl
A rapid staining-assisted wood sampling method for PCR-based detection of pine wood nematode Bursaphelenchus xylophilus in Pinus massoniana wood tissue
For reasons of unequal distribution of more than one nematode species in wood, and limited
availability of wood samples required for the PCR-based method for detecting pinewood nematodes in
wood tissue of Pinus massoniana, a rapid staining-assisted wood sampling method aiding PCR-based
detection of the pine wood nematode Bursaphelenchus xylophilus (Bx) in small wood samples of P.
massoniana was developed in this study. This comprised a series of new techniques: sampling, mass
estimations of nematodes using staining techniques, and lowest limit Bx nematode mass determination
for PCR detection. The procedure was undertaken on three adjoining 5-mg wood cross-sections, of
0.5 · 0.5 · 0.015 cm dimension, that were cut from a wood sample of 0.5 · 0.5 · 0.5 cm initially, then
the larger wood sample was stained by acid fuchsin, from which two 5-mg wood cross-sections (that
adjoined the three 5-mg wood cross-sections, mentioned above) were cut. Nematode-staining-spots
(NSSs) in each of the two stained sections were counted under a microscope at 100· magnification. If
there were eight or more NSSs present, the adjoining three sections were used for PCR assays. The
B. xylophilus – specific amplicon of 403 bp (DQ855275) was generated by PCR assay from 100.00% of
5-mg wood cross-sections that contained more than eight Bx NSSs by the PCR assay. The entire
sampling procedure took only 10 min indicating that it is suitable for the fast estimation of nematode
numbers in the wood of P. massonina as the prelimary sample selections for other more expensive
Bx-detection methods such as PCR assay
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