26 research outputs found

    A Systematic Analysis on DNA Methylation and the Expression of Both mRNA and microRNA in Bladder Cancer

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    Background: DNA methylation aberration and microRNA (miRNA) deregulation have been observed in many types of cancers. A systematic study of methylome and transcriptome in bladder urothelial carcinoma has never been reported. Methodology/Principal Findings: The DNA methylation was profiled by modified methylation-specific digital karyotyping (MMSDK) and the expression of mRNAs and miRNAs was analyzed by digital gene expression (DGE) sequencing in tumors and matched normal adjacent tissues obtained from 9 bladder urothelial carcinoma patients. We found that a set of significantly enriched pathways disrupted in bladder urothelial carcinoma primarily related to "neurogenesis" and "cell differentiation" by integrated analysis of -omics data. Furthermore, we identified an intriguing collection of cancer-related genes that were deregulated at the levels of DNA methylation and mRNA expression, and we validated several of these genes (HIC1, SLIT2, RASAL1, and KRT17) by Bisulfite Sequencing PCR and Reverse Transcription qPCR in a panel of 33 bladder cancer samples. Conclusions/Significance: We characterized the profiles between methylome and transcriptome in bladder urothelial carcinoma, identified a set of significantly enriched key pathways, and screened four aberrantly methylated and expressed genes. Conclusively, our findings shed light on a new avenue for basic bladder cancer research

    Legislative Documents

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    Also, variously referred to as: Senate bills; Senate documents; Senate legislative documents; legislative documents; and General Court documents

    Legislative Documents

    No full text
    Also, variously referred to as: Senate bills; Senate documents; Senate legislative documents; legislative documents; and General Court documents

    Supplementary Material, Table-S4 – Regulation of Pol II Pausing Is Involved in Daily Gene Transcription in the Mouse Liver

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    <p>Supplementary Material, Table-S4 for Regulation of Pol II Pausing Is Involved in Daily Gene Transcription in the Mouse Liver by Jialou Zhu, Chengwei Li, Changxia Gong, and Xiaodong Li in Journal of Biological Rhythms</p

    Supplementary Material, Fig-S2 – Regulation of Pol II Pausing Is Involved in Daily Gene Transcription in the Mouse Liver

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    <p>Supplementary Material, Fig-S2 for Regulation of Pol II Pausing Is Involved in Daily Gene Transcription in the Mouse Liver by Jialou Zhu, Chengwei Li, Changxia Gong, and Xiaodong Li in Journal of Biological Rhythms</p

    Supplementary Material, Fig-S3 – Regulation of Pol II Pausing Is Involved in Daily Gene Transcription in the Mouse Liver

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    <p>Supplementary Material, Fig-S3 for Regulation of Pol II Pausing Is Involved in Daily Gene Transcription in the Mouse Liver by Jialou Zhu, Chengwei Li, Changxia Gong, and Xiaodong Li in Journal of Biological Rhythms</p

    Supplementary Material, Fig-S7 – Regulation of Pol II Pausing Is Involved in Daily Gene Transcription in the Mouse Liver

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    <p>Supplementary Material, Fig-S7 for Regulation of Pol II Pausing Is Involved in Daily Gene Transcription in the Mouse Liver by Jialou Zhu, Chengwei Li, Changxia Gong, and Xiaodong Li in Journal of Biological Rhythms</p

    Supplementary Material, Table-S6_(1) – Regulation of Pol II Pausing Is Involved in Daily Gene Transcription in the Mouse Liver

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    <p>Supplementary Material, Table-S6_(1) for Regulation of Pol II Pausing Is Involved in Daily Gene Transcription in the Mouse Liver by Jialou Zhu, Chengwei Li, Changxia Gong, and Xiaodong Li in Journal of Biological Rhythms</p

    Supplementary Material, Table-S3 – Regulation of Pol II Pausing Is Involved in Daily Gene Transcription in the Mouse Liver

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    <p>Supplementary Material, Table-S3 for Regulation of Pol II Pausing Is Involved in Daily Gene Transcription in the Mouse Liver by Jialou Zhu, Chengwei Li, Changxia Gong, and Xiaodong Li in Journal of Biological Rhythms</p
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