Bacteriological and Molecular Identification of Bartonella Species in Cats from Different Regions of China

With the improvements in diagnostic techniques, Bartonella henselae (B. henselae) infection has recently been recognized to cause a widening spectrum of diseases. Cats are the natural reservoir hosts of B. henselae. The current study aims to investigate the prevalence of B. henselae infection in the cat populations in China. Polymerase chain reaction (PCR) and bacterial cultures confirm that 12.7% of the tested cats were positive for the infection. Old age and outdoor exposure were statistically associated with the infection. Multilocus sequence typing and eBURST analysis of the cat isolates collected in the present study show that 65.4% of the isolates belong to sequence type 1 (ST1). Three new STs (ST16–18) were identified in Midwestern China. These results may aid our understanding of the population structure of B. henselae in China and the relationship between human and cat strains in subsequent studies

Study on sandstorm in the western section of Hexi Corridor of 2020

A diagnostic analysis of the dust storm weather occurring in the western part of the Hexi Corridor from 27-30 June 2020 was carried out using Jiuquan city’s live data, as well as ECMWF numerical forecasts, and the NCEP reanalysis information was used to draw a theta dust storm model based on the routing principle. The results show that: 1.In the early stage of sandstorm, there was no precipitation in Suzhou and Jinta counties in March and April, which resulted in dry surface and thickened soil layer. These factors created favorable conditions for the occurrence of dust weather. 2.Development of unstable trough eastward in front of the ridge of Mountain Ural at 500 hPa, The cold high pressure is continuously strengthened in the process of moving, which is the large-scale system leading to the sandstorm. Thermal depression in front of ground cold front is the main cause for the outbreak of the severe sandstorm. 3.The beginning and ending time of the sandstorm can be accurately predicted by the simulation chart of sandstorm forecast, which has a good indication significance for the sandstorm forecast in the Hexi area

Correlation of 3'-phosphoadenosine-5'-phosphosulfate synthase 1 (PAPSS1) expression with clinical parameters and prognosis in esophageal squamous cell carcinoma

Background. In recent years, 3'- phosphoadenosine-5'-phosphosulfate synthase 1 (PAPSS1) has been found to be highly expressed in some cancers and significantly associated with prognosis. Nevertheless, the role of PAPSS1 in esophageal squamous cell carcinoma (ESCC) is poorly understood. Methods. In this study, PAPSS1 expression in ESCC samples was researched through real-time quantitative polymerase chain reaction (qPCR), immunohistochemistry (IHC), and western blot (WB) techniques. siRNA technology was then used to inhibit PAPSS1 expression in ESCC cells, and cytologic tests were conducted to research gene affection on cell apoptosis, proliferation, and migration. Then, the expression of Bcl2, Ki67, and Snail was detected using qPCR and WB tests. These experimental data were analyzed by GraphPad software, where the P-value <0.05 was statistically significant. Results. The results showed that PAPSS1 expression level in ESCC tissues was higher than in the adjacent tissues. The data also showed that PAPSS1 was significantly correlated with N stage, and that the patients with high expressions had longer survival time. After transfection for 48 hours, the cell apoptosis rate of siRNA-PAPSS1 transfected groups decreased significantly, whereas the cell proliferation rate and migration ability increased relative to the control. At the same time, the expression levels of Bcl2, Ki67 and Snail were all upregulated by siRNA-PAPSS1. PAPSS1, however, was suppressed. Conclusions. PAPSS1 may be an ESCC suppressor gene, and its specific molecular mechanism in ESCC needs to be further studied

Investigation and analysis of the mental pressure of outpatient medical attendants and nurses during the COVID-19 pandemic in china

Certain sicknesses or contaminations influence an enormous number of individuals in a limited capacity to focus time. A neighbourhood endemic illness can flare-up into a scourge influencing the entire populace or district which on occasion reach out to different nations and mainlands and become pandemic. Pandemics brings about death toll just like the economy. Pooled endeavors and assets, compelling sharing of information, equal numerous methodologies just as the physical and mental condition of forefront staff impact the board of pandemics. The COVID illness COVID-19 brought about by SARS-CoV-2 began in Dec 2019 from Wuhan in China, is currently an overall general wellbeing crisis influencing a huge number of people. It influences numerous cutting edge medical care laborers as well. Here, we contemplated mental pressure and proficient personality of Nurses and Staff for potential relationships, assuming any, and break down affecting elements. We utilized a purposive testing method with 415 Nurses and Staff in Nanjing, China through an overall data poll, seen pressure scale, and nursing proficient personality survey. Attendants and Staff' inception, month to month everyday costs, and their insight on plague counteraction and treatment have indicated a critical effect on their mental pressure (P&lt; 0.010). Essentially, Nurse's and Staff's sexual orientation, inception, clinical practices, and information on counteraction and treatment, and whether they effectively learn such information sway altogether on their expert personality (P&lt; 0.010). The general score of mental pressure were (24.470±07.350) and proficient personality had 72.470±08.070. The pressure condition showed a negative connection with the level of expert character (P&lt; 0.01, r = 00.457). Expanded mental pressure, had a lower feeling of expert personality. Generally speaking, the examination of information on saw pressure and expert character pandemic proposes that feelings of anxiety are contrarily relative to information in compelling methods of taking care of the pandemic. Attendants and Staff with clinical practice fared better as far as expert character. The examination proposes Nurses and Staff to remain zeroed in on investigations, clinical practice, and directing, whenever required

Investigation and analysis of the mental pressure of outpatient medical attendants and nurses during the COVID-19 pandemic in china

261-271Certain sicknesses or contaminations influence an enormous number of individuals in a limited capacity to focus time. A neighbourhood endemic illness can flare-up into a scourge influencing the entire populace or district which on occasion reach out to different nations and mainlands and become pandemic. Pandemics brings about death toll just like the economy. Pooled endeavors and assets, compelling sharing of information, equal numerous methodologies just as the physical and mental condition of forefront staff impact the board of pandemics. The COVID illness COVID-19 brought about by SARS-CoV-2 began in Dec 2019 from Wuhan in China, is currently an overall general wellbeing crisis influencing a huge number of people. It influences numerous cutting edge medical care laborers as well. Here, we contemplated mental pressure and proficient personality of Nurses and Staff for potential relationships, assuming any, and break down affecting elements. We utilized a purposive testing method with 415 Nurses and Staff in Nanjing, China through an overall data poll, seen pressure scale, and nursing proficient personality survey. Attendants and Staff' inception, month to month everyday costs, and their insight on plague counteraction and treatment have indicated a critical effect on their mental pressure (PPP< 0.01, r = -00.457). Expanded mental pressure, had a lower feeling of expert personality. Generally speaking, the examination of information on saw pressure and expert character pandemic proposes that feelings of anxiety are contrarily relative to information in compelling methods of taking care of the pandemic. Attendants and Staff with clinical practice fared better as far as expert character. The examination proposes Nurses and Staff to remain zeroed in on investigations, clinical practice, and directing, whenever required

Magnetic resonance imaging features of bile duct adenoma

ObjectivesTo evaluate the magnetic resonance imaging (MRI) features of bile duct adenoma.MethodsThe data of 28 patients [with 32 pathologically confirmed bile duct adenomas, including 15 with malignant change (malignant group) and 17 without malignant change (benign adenoma group)] were retrospectively reviewed. Abdominal MRI was performed for all patients; in addition, dynamic enhanced MRI was performed for 18 lesions. The MRI features, including lesion location, maximum size, morphology, signal characteristics, enhancement type, and appearance of the bile duct, were assessed by two abdominal radiologists. Apparent diffusion coefficient (ADC) values were measured and compared.ResultsOf the 32 bile duct adenomas, 22 (68.75%) involved the common bile duct (CBD). While 14/32 (43.75%) lesions presented as focal eccentric-type masses, 9/32 (28.13%) presented as plaque-like masses, 4/32 (12.50%) as bile duct casting masses, and 5/32 (15.62%) as infiltrative masses. A frond-like superficial appearance was seen in 8/32 (25%) lesions. Infiltrative masses were significantly more common in the malignant group than in the benign adenoma group (P = 0.015). While 23/32 (71.88%) lesions were isointense on T1-weighted imaging (T1WI), 24/32 (75%) were hyperintense on T2-weighted imaging (T2WI). Bile duct dilatation was present upstream of the lesion in all cases. Bile duct dilatation at the lesion was seen in 24/32 (75%) cases and downstream of the lesion in 6/32 (18.75%) cases. Of the 18 lesions that underwent dynamic enhanced MRI, 14 (77.78%) showed moderate enhancement and 13 (72.22%) showed persistent enhancement. On diffusion-weighted imaging (DWI), 27/32 (84.37%) lesions showed hyperintensity. Mean ADC value was comparable between the malignant group and the benign adenoma group (P = 0.156).ConclusionsBile duct adenoma primarily presents as intraductal growth in the CBD, usually with bile duct dilatation at the lesion site or upstream to it. Most lesions are isointense on T1WI, are hyperintense on T2WI and DWI, and show moderate enhancement. A superficial frond-like appearance of the lesion and bile duct dilatation at the lesion or downstream to it might be characteristics of bile duct adenoma. An infiltrative appearance might indicate malignant transformation

Compound heterozygosity for novel truncating variants in the LMOD3 gene as the cause of polyhydramnios in two successive fetuses

Polyhydramnios is sometimes associated with genetic defects. However, establishing an accurate diagnosis and pinpointing the precise genetic cause of polyhydramnios in any given case represents a major challenge because it is known to occur in association with over 200 different conditions. Whole exome sequencing (WES) is now a routine part of the clinical workup, particularly with diseases characterized by atypical manifestations and significant genetic heterogeneity. Here we describe the identification, by means of WES, of novel compound heterozygous truncating variants in the LMOD3 gene [i.e., c.1412delA (p.Lys471Serfs*18) and c.1283dupC (p.Gly429Trpfs*35)] in a Chinese family with two successive fetuses affected with polyhydramnios, thereby potentiating the prenatal diagnosis of nemaline myopathy (NM) in the proband. LMOD3 encodes leiomodin-3, which is localized to the pointed ends of thin filaments and acts as a catalyst of actin nucleation in skeletal and cardiac muscle. This is the first study to describe the prenatal and postnatal manifestations of LMOD3-related NM in the Chinese population. Of all the currently reported NM-causing LMOD3 nonsense and frameshifting variants, c.1412delA generates the shortest truncation at the C-terminal end of the protein, underscoring the critical role of the WH2 domain in LMOD3 structure and function. Survey of the prenatal phenotypes of all known LMOD3-related severe NM cases served to identify fetal edema as a novel presenting feature that may provide an early clue to facilitate prenatal diagnosis of the disease