学会抄録

Tumor type and common subtype classification of Achilles cell lines. (XLSX 16 kb

Additional file 8: Figure S4. of Outlier analysis of functional genomic profiles enriches for oncology targets and enables precision medicine

The ATARiS gene level score distribution for (A) EGFR and (B) ERBB2. A probability density estimate is computed by Gaussian kernel smoothing. (DOCX 49 kb

Additional file 4: Table S2. of Outlier analysis of functional genomic profiles enriches for oncology targets and enables precision medicine

The complete list of genes with outlier patterns along with tumor type indication and related predictive biomarker results (sorted by decreasing statistical significance of association), if any. The table indicates for each gene which of the three analysis methods determined it to be associated with an outlier responder pattern. (XLSX 16 kb

Effect of IL-22 (CC10-IL-22) on cytokine and chemokine production in OVA-induced allergic asthma.

<p>Th1 cytokine, IFN-γ, and Th2 cytokines, IL-4 and IL-13, Th17 cytokine IL-17A, and chemokine eotaxin in the BAL were measured by ELISA. The number of animals in each group was indicated and data were shown as Mean±SEM. **<i>P</i><0.01 (unpaired Student t-test).</p

IL-22 attenuated airway hyperresponsiveness (AHR).

<p>(A, B) Invasive PFT of OVA stimulated IL-22 Tg(+) and Tg(−) mice was assessed (FlexiVent, SciQuest). Lung resistance at baseline and in response to increasing concentrations of methacholine (MCh) through inhalation was recorded and analyzed (*<i>P</i><0.05). The number of animals used in each group was as indicated. Data represented as Mean±SEM. OVA-induced IL-22 Tg(+) mice showed significantly lower lung resistance compared to OVA-induced IL-22 Tg(−) mice.</p

Serum immunoglobulin levels in OVA-induced allergic asthma in IL-22 Tg(+) (SPC-IL-22) and Tg(−) mice.

<p>Serum samples from IL-22 Tg(+) and Tg(−) mice were collected 48 hours after last OVA challenge. Immunoglobulins, including total and OVA-specific IgE, IgG1 and IgG2a were measured by ELISA and analyzed by one-way ANOVA. Data from individual animals were plotted. Both IL-22 Tg(+) and Tg(−) group showed much higher level either in total or in OVA-specific IgE, IgG1 and IgG2a than PBS treatment groups (<i>P</i><0.01). But there is no difference between IL-22 Tg(+) and Tg(−) groups (<i>P</i>>0.05).</p

IL-22 alleviated OVA-induced eosinophilic inflammation in the lung.

<p>(A) High levels of IL-22 cytokine were seen in the BAL of PBS and OVA-stimulated IL-22 Tg(+) mice without difference between the two groups (<i>P</i>>0.05). When compared to Tg(−) mice, IL-22 concentrations in the BAL of Tg(+) mice were much higher than that in PBS and OVA-stimulated control groups (<i>P</i><0.0001). (B, C) BAL total cell and differentials counts showed that OVA-stimulated IL-22 Tg(+) group had a much higher percentage of eosinophils compared to OVA-stimulated Tg(−) mice (<i>P</i><0.0001), but there is no difference in the total cell counts (<i>P</i>>0.05). (D) Lung histology of OVA-induced allergic asthma in SPC-IL-22 Tg(+) mice and Tg(−) mice, H&E, IHC for MBP, and Alcian blue staining showed that OVA-induced IL-22 Tg(−) group had much more severe airway inflammation compared to OVA-induced IL-22 Tg(+) group.</p

Effect of IL-22 (SPC-IL-22) on OVA-induced systemic and local immune responses.

<p>Splenocytes and lymphocytes from peribronchial draining lymph nodes (DLN) from IL-22 Tg(+) and Tg(−) mice after OVA challenge were cultured and stimulated with medium control, OVA or CD3/CD28. Th1 cytokine, IFN-γ, and Th2 cytokine, IL-13 in the supernatant were measured by ELISA. The number of animals used in the experiments was indicated and data were shown as Mean±SEM. *<i>P</i><0.05 and **<i>P</i><0.01 (unpaired Student t-test).</p

Histology of allergic rhinitis and <i>mev</i> mice.

<p>(A) Hematoxylin and eosin stains of the nasal cavity from controls, allergic rhinitis (OVA) and <i>mev</i> mice. (B) In allergic and <i>mev</i> mice, there is clearly increased nasal inflammation with eosinophilia, as compared to controls. (C) Epithelial thickness significantly increased in allergic mice compared with controls and <i>mev</i> mice (*<i>p</i><0.05, **<i>p</i><0.01, and ***<i>p</i><0.001).</p

Relative mRNA expression of Th2 cytokines (IL-4 and IL-13) in controls, allergic rhinitis (OVA), and <i>mev</i> mice (*<i>p</i><0.05 and ***<i>p</i><0.001).

<p>IL-4 is significantly up-regulated in both allergic rhinitis and <i>mev</i> mice compared with controls. However, IL-13 significantly increased only in OVA model.</p