Preservation of Supported Lipid Membrane Integrity from Thermal Disruption: Osmotic Effect

Preservation of structural integrity under various environmental conditions is one major concern in the development of the supported lipid membrane (SLM)-based devices. It is common for SLMs to experience temperature shifts from manufacture, processing, storage, and transport to operation. In this work, we studied the thermal adaption of the supported membranes on silica substrates. Homogenous SLMs with little defects were formed through the vesicle fusion method. The mass and fluidity of the bilayers were found to deteriorate from a heating process but not a cooling process. Fluorescence characterizations showed that the membranes initially budded as a result of heating-induced lipid lateral area expansion, followed by the possible fates including maintenance, retraction, and fission, among which the last contributes to the irreversible compromise of the SLM integrity and spontaneous release of the interlipid stress accumulated. Based on the mechanism, we developed a strategy to protect SLMs from thermal disruption by increasing the solute concentration in medium. An improved preservation of the membrane mass and fluidity against the heating process was observed, accompanied by a decrease in the retraction and fission of the buds. Theoretical analysis revealed a high osmotic energy penalty for the fission, which accounts for the depressed disruption. This osmotic-based protection strategy is facile, solute nonspecific, and long-term efficient and has little impact on the original SLM properties. The results may help broaden SLM applications and sustain the robustness of SLM-based devices under multiple thermal conditions

Effect of Osmotic Stress on Membrane Fusion on Solid Substrate

There is currently a lack of comprehensive understanding of osmotic effect on lipid vesicle fusion on solid oxide surface. The question has both biological and biomedical implications. We studied the effect by quartz crystal microbalance with dissipation monitoring using NaCl, sucrose as osmolytes, and two different osmotic stress imposition methods, which allowed us to separate the osmotic effects from the solute impacts. Osmotic stress was found to have limited influence on the fusion kinetics, independently of the direction of the gradient. Further atomic force microscopy experiments and energy consideration implied that osmotic stress spends the majority of chemical potential energy associated in directed transport of water across membrane. Its contribution to vesicle deformation and fusion on substrate is therefore small compared to that of adhesion