Deep tillage reduces the dependence of tobacco on AMF and promotes the growth of tabacco(Nicotiana tabacum L.) in dryland

The traditional shallow tillage method makes the soil quality declining, and affects the efficiency of agricultural production. Taking the conventional rotary tillage(12 cm) as the control, Yunyan 87 as the test variety, and the soil type of the test site is paddy soil, we studied the effects of deep tillage(Subsoiling 30 cm) on soil nutrients, arbuscular mycorrhizal fungi (AMF) and tobacco（Nicotiana tabacum L.）growth. The results showed that deep tillage increased the contents of organic carbon, available phosphorus(AP) and available potassium(AK) in 20 ~ 40 cm soil layer. The community of AMF was changed by deep tillage. Glomus, the dominant genus in both group, increased significantly in the soil after deep tillage. The colonization rate of AMF was lower than that of conventional rotary tillage. Deep tillage was beneficial to the growth of tobacco in the middle and late stages. Root growth and nutrient content of tobacco increased. Deep tillage significantly improved the output value of tobacco. It can be seen that deep tillage is conducive to improving soil fertility, promoting the vigorous growth of root, reducing the dependence of tobacco on AMF, and promoting the high quality and yield of tobacco in drylands of Hunan.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Additional file 1 of The succession pattern of soil microbial communities and its relationship with tobacco bacterial wilt

Figure S1. Rarefaction curves of 16r RNA gene sequencing data. Figure S2. Composition and structure of soil microbial communities in each group. Figure S3. Phylogenetic molecular ecological networks (pMEN) of microbial communities in each group, and number of nodes and links of each pMEN. Table S1. Soil properties. Table S2(a). Correlation of microbial populations in abundance between two periods at the phylum level. (b). Correlation of microbial populations in abundance between two periods at the genus level. Table S3. Topological properties of the empirical pMENs of microbial communities in eight groups. Table S4(a). Correlation between abundance of microbial populations and tobacco morbidity at the phylum level. (b). Correlation between abundance of microbial populations and tobacco morbidity at the genus level. Table S5(a). Mantel test of sequencing data with environmental attributes at the phylum level. (b). Mantel test of sequencing data with environmental attributes at the genus level. (DOCX 1100 kb

Responses of Tobacco Growth and Development, Nitrogen Use Efficiency, Crop Yield and Economic Benefits to Smash Ridge Tillage and Nitrogen Reduction

Long-term rotary tillage leads to thinning of the soil layer and low nitrogen use efficiency of crops, resulting in a decrease in crop yield and quality. Therefore, a new alternative method to improve nitrogen use efficiency of crops needs to be found urgently. Here, we analyzed the effects of a new smash ridge tillage method combined with reduced nitrogen application on tobacco growth and development, and nitrogen use efficiency and its economic benefits. The results showed that, compared with conventional tillage and nitrogen application of 180 kg N ha−1, smash ridge tillage and a 30% reduction in nitrogen rate resulted in greater root length density, more primary lateral roots and greater rooting depth in the subsoil. It is also beneficial to maintain a high level of biomass and nitrogen accumulation in the later growth period, increasing the output value of tobacco by CNY 1588.35 ha−1 and reducing the cost by CNY 974.1 ha−1 on average in two years. In conclusion, our study highlights the economic benefits of smash ridge tillage and nitrogen reduction for tobacco growth and development, and considers them an effective method for improving agricultural productivity and nitrogen use efficiency