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    Additional file 1 of Integrating spatial transcriptomics and single-cell RNA-sequencing reveals the alterations in epithelial cells during nodular formation in benign prostatic hyperplasia

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    Additional file1. Table S1. General information of samples used for Immunofluorescent staining. Table S2. General information of samples used for Immunohistochemical staining. Table S3. General information of samples used for ST and single cell RNA sequencing. Figure S1. The atlas of single-cell and spatial transcriptomics in prostate tissues (n=13). Figure S2. Subsets analysis of epithelial cells. Figure S3. Characteristic of BE5 subgroup in BPH tissue. Figure S4. Localization and expression of FOS within BPH tissues. Figure S5. Bar plot illustrating the percentage of positive (100-negative) c-Fos expression in both IPP>10mm and IPP≤10mm BPH patients. Figure S6. Dot plots of dynamic expression of top four DEGs along two cell fates at the branch point 3 in the pseudo-time cell trajectory for the normal, BPH_GN, and BPH_SN BE5 cells
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