Immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella pneumonia bacteremia.

Incidences of leukopenia caused by bacteremia have increased significantly and it is associated with prolonged hospital stay and increased cost. Immunoproteomic is a promising method to identify pathogenicity factors of different diseases. In the present study, we used immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella Pneumonia bacteremia. Approximately 40 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 170 kDa were identified. Pathogenicity factors including S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor tu (EF-Tu). In validation of the pathogenicity factor, we used western blotting to show that Klebsiella pneumonia had higher (EF-Tu) expression when they accompanied by leukopenia rather than leukocytosis. Thus, we report 6 pathogenicity factors of leukopenia caused by Klebsiella pneumonia bacteremia, including 5 housekeeping enzymes and EF-Tu. We suggest EF-Tu could be a potential pathogenicity factor for leukopenia caused by Klebsiella pneumonia

Diagnostic value of galactomannan detection in bronchoalveolar lavage fluid in COVID-19 associated invasive pulmonary fungal infection

Objective To analyze the concentration of galactomannan (GM) in bronchoalveolar lavage fluid (BALF) and its value in the diagnosis of COVID-19 related invasive pulmonary fungal infection(IPFI). Methods From 2022 to 2023, 41 patients with COVID-19 related IPFI confirmed, clinically diagnosed or to be diagnosed in the First Affiliated Hospital of Anhui Medical University were taken as the experimental group, and 42 patients with simple pneumonia were taken as the control group. Bronchoscopic alveolar lavage was performed in both groups, and the contents of GM in both BALF and serum were detected at the same time. The concentration difference and clinical diagnostic value of GM between the two groups of different specimens were analyzed, and ROC was used to evaluate the diagnostic efficacy. Results Compared with the control group, the experimental group had higher BALF GM concentrations [（2.27±1.78） ng/L vs (0.51±0.28) ng/L, t =6.329, P＜0.01) and serum GM concentrations [(1.57±0.56) ng/L vs (0.32±0.11) ng/L, t =14.190, P＜0.01＝. The optimal cut-off value for diagnosing IPFI with BALF was 1.82 ng/L, with sensitivity and specificity of 77.9% and 89.2%, respectively, and AUC of 0.92 (95%CI: 0.871-0.955). The optimal cut-off value for serum was 0.67 ng/L, with sensitivity and specificity of 64.9% and 92.2%, respectively, and AUC of 0.89 (95%CI: 0.854-0.925). However, the false positive rate of BALF was slightly higher than that of serum (10.8% vs 7.8%). Conclusion GM can be serve of as a good indicator for evaluating IPFI related to COVID-19. The GM detection in BALF has more clinical value in the diagnosis of IPFI than serum

2-DE analysis of proteins from Klebsiella pneumonia in leukocyte patients caused by Klebsiella pneumonia bacteremia.

<p>Detection of pathogenicity factors by Western blot analysis in CD patients. The six protein spots are labeled with arrows.</p

Differentially Expressed Proteins Identified by MALDI-TOF-MS.

<p>Differentially Expressed Proteins Identified by MALDI-TOF-MS.</p

Western blot analysis to validate the reactive proteins.

<p>Klebsiella pneumonia from Klebsiella pneumonia bacteremia patients with (<b>A</b>) leukopenia and (<b>B</b>) leukocytosis were respectively examined by Western blot analysis to validate the reactive proteins ef-tu. Note the higher expression levels of ef-tu in patients with leukopenia caused by Klebsiella pneumonia.</p

Passive Deicing CFRP Surfaces Enabled by Super-Hydrophobic Multi-Scale Micro-Nano Structures Fabricated via Femtosecond Laser Direct Writing

Carbon fiber reinforced plastic (CFRP) is the main material of aircraft skin. Preparing superhydrophobic anti-icing/deicing surface on the CFRP is of great importance for aircraft flight safety. In this work, a variety of multi-scale micro-nano structures were imprinted on CFRP by femtosecond laser processing, and a transition from hydrophilic to superhydrophobic CFRP was realized. After being optimized by different geometries and laser conditions, the water contact angle, which is tested at 24.3 °C and 34% humidity, increased from 88 ± 2° (pristine) to 149 ± 3° (100 μm groove) and 153 ± 3° (80 μm grid). A further anti-icing test at −10 °C (measured on the cooling platform) and 28% humidity showed that the freezing time was increased from 78 ± 10 s (pristine) to 282 ± 25 s (80 μm grid). Most importantly, the tensile tests showed that the femtosecond laser processing method did not deteriorate the mechanical properties of CFRP. This work provides great significance for aircraft passive deicing technology