Unveiling the novel immune and molecular signatures of ovarian cancer: insights and innovations from single-cell sequencing

Ovarian cancer is a highly heterogeneous and lethal malignancy with limited treatment options. Over the past decade, single-cell sequencing has emerged as an advanced biological technology capable of decoding the landscape of ovarian cancer at the single-cell resolution. It operates at the level of genes, transcriptomes, proteins, epigenomes, and metabolisms, providing detailed information that is distinct from bulk sequencing methods, which only offer average data for specific lesions. Single-cell sequencing technology provides detailed insights into the immune and molecular mechanisms underlying tumor occurrence, development, drug resistance, and immune escape. These insights can guide the development of innovative diagnostic markers, therapeutic strategies, and prognostic indicators. Overall, this review provides a comprehensive summary of the diverse applications of single-cell sequencing in ovarian cancer. It encompasses the identification and characterization of novel cell subpopulations, the elucidation of tumor heterogeneity, the investigation of the tumor microenvironment, the analysis of mechanisms underlying metastasis, and the integration of innovative approaches such as organoid models and multi-omics analysis

A Throughput Performance Analysis Method for Multimode Underwater Acoustic Communication Network Based on Markov Decision Process

The multimode underwater acoustic communication network is a novel form of underwater acoustic communication that adjusts its communication mode to enhance overall performance. Current performance analysis methods are primarily applied to single-mode networks and assume uniform communication capability across all nodes, making them unsuitable for multimode networks. This paper investigates the multimode communication of the physical layer, considering factors such as the marine environment, the node transmitting sound source level, and the transmitting distance. A decoding conflict model is proposed to support multimode concurrent transmission scenarios. The communication mode is designed to be compatible with the channel and node characteristics. Additionally, using a Markov decision process, this paper establishes a performance evaluation and analysis model for multimode underwater acoustic networks to determine throughput performance limits in real underwater environments. Simulations across various scenarios validate that the throughput performance limits obtained by this method are more accurate under multimode networks, with an improvement in accuracy of over 67.5% compared to existing methods

Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique

Abstract Background Ralstonia eutropha is an important bacterium for the study of polyhydroxyalkanoates (PHAs) synthesis and CO2 fixation, which makes it a potential strain for industrial PHA production and attractive host for CO2 conversion. Although the bacterium is not recalcitrant to genetic manipulation, current methods for genome editing based on group II introns or single crossover integration of a suicide plasmid are inefficient and time-consuming, which limits the genetic engineering of this organism. Thus, developing an efficient and convenient method for R. eutropha genome editing is imperative. Results An efficient genome editing method for R. eutropha was developed using an electroporation-based CRISPR-Cas9 technique. In our study, the electroporation efficiency of R. eutropha was found to be limited by its restriction-modification (RM) systems. By searching the putative RM systems in R. eutropha H16 using REBASE database and comparing with that in E. coli MG1655, five putative restriction endonuclease genes which are related to the RM systems in R. eutropha were predicated and disrupted. It was found that deletion of H16_A0006 and H16_A0008-9 increased the electroporation efficiency 1658 and 4 times, respectively. Fructose was found to reduce the leaky expression of the arabinose-inducible pBAD promoter, which was used to optimize the expression of cas9, enabling genome editing via homologous recombination based on CRISPR-Cas9 in R. eutropha. A total of five genes were edited with efficiencies ranging from 78.3 to 100%. The CRISPR-Cpf1 system and the non-homologous end joining mechanism were also investigated, but failed to yield edited strains. Conclusions We present the first genome editing method for R. eutropha using an electroporation-based CRISPR-Cas9 approach, which significantly increased the efficiency and decreased time to manipulate this facultative chemolithoautotrophic microbe. The novel technique will facilitate more advanced researches and applications of R. eutropha for PHA production and CO2 conversion

Drug-free in vitro activation combined with ADSCs-derived exosomes restores ovarian function of rats with premature ovarian insufficiency

Abstract Background Drug-free in vitro activation (IVA) is a new protocol to activate residual dormant follicles for fertility restoration in patients with premature ovarian insufficiency (POI). However, several deficiencies have reduced its clinical efficacy rate. Our previous studies have confirmed that the combination of adipose-derived stem cells (ADSCs) and drug-free IVA can improve the effectiveness of drug-free IVA and restore ovarian function of rats with POI. Increasing evidence has demonstrated that mesenchymal stem cell-derived exosomes have similar therapeutic effects as their source cells. Here, we performed a preclinical study to evaluate the therapeutic effects of ADSCs-derived exosomes (ADSCs-Exos) combined with drug-free IVA in the POI rats and the mechanism in restoring ovarian function. Results In vivo, the effects of ADSCs-Exos were comparable to those of ADSCs, and the ADSCs-Exos combined with drug-free IVA was better than ADSCs-Exos alone therapy in promoting follicular development. Moreover, transplantation of ADSCs/ADSCs-Exos lead to up-regulation of BCL-2 expression and down-regulation of the expression of Bax and Cleaved Caspase-3, thus reducing the apoptosis of chemotherapy-induced follicle cells, and further promoting the development of the follicles and rescuing ovarian function in POI-damaged ovary. In vitro, ovarian fragmentation could activate follicular growth and development, and in combination with ADSCs-Exos could prevent the loss of follicles, promote follicular proliferation and inhibit apoptosis. Conclusions ADSCs-Exos combined drug-free IVA had remarkable therapeutic effects in restoring ovarian function of POI rats, and markedly promoted follicular development and inhibited apoptosis of ovarian cells in vitro. Our study confirmed that the combination therapy might be a promising and effective treatment for POI

Detection System for U-Shaped Bellows Convolution Pitches Based on a Laser Line Scanner

An expansion joint is mainly composed of bellows and other components; it is attached on a container shell or pipe to compensate for the additional stress caused by temperature differences and mechanical vibrations. In China, the expansion joint fatigue tests are often used to assess the quality of products. After fatigue tests, convolution pitch will be changed. The amount of change is an important index that can be used to evaluate bellows expansion joints. However, the convolution pitch detection is mainly done manually and randomly by inspection agencies before shipping to the end users. This common practice is not efficient and is often subjective. This paper introduced a novel method for automatically detecting the change of the convolution pitch based on a laser line scanner and data processing technology. The laser line scanner is combined with a precision motorized stage to obtain the point cloud data of the bellows. After denoising and fitting, a peak-finding algorithm is applied to search for the crest of a convolution. The method to find the convolution pitch and the decision that needs to be made to ensure product eligibility are described in detail. A DN500 expansion joint is used as a sample to illustrate the efficiency of the system. The application of the technique intuitively allows a higher precision and relative efficiency in quality inspection of bellows expansion joints. It has also been implemented in the Special Equipment Safety Supervision and Inspection Institute of Jiangsu province with great success

Evolution Process of Ancient Landslide Reactivation under the Action of Rainfall: Insights from Model Tests

Under rapid global climate change, the risk of ancient landslide reactivation induced by rainfall infiltration is increasing significantly. The contribution of cracks to the reactivation of ancient landslides, as an evolutionary product, is a topic that deserves attention; however, current research on this issue remains insufficient. In this study, taking the Woda landslide in the upper Jinsha River as a case study, we investigated the reactivation mechanisms of ancient landslides with and without cracks under rainfall based on model tests. The study showed that cracks influence the reactivation range and depth of ancient landslide. In cases where no cracks develop on ancient landslides, rainfall can only cause shallow sliding with failure concentrated at its front edge. Conversely, when cracks develop on ancient landslides, rainwater can quickly infiltrate into the sliding zone along the cracks and induce overall reactivation of the ancient landslide. Furthermore, the reactivation mechanism of ancient landslides without cracks is that the failure of ancient landslide foot results in progressive failure at the front of the ancient landslide. When cracks have developed at ancient landslides, the reactivation mechanism of which involves mid-rear ancient landslide creeping, tensile cracks develop on the mid-rear ancient landslide, with localized sliding at the front edge, tensile cracks extending, local sliding range extending, accelerated creeping, and progressive failure of the mid-rear ancient landslide. These findings shed light on how cracks influence rainfall-induced mechanisms of ancient landslide reactivation and hold great significance for advancing our understanding regarding these mechanisms

MOESM1 of Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique

Additional file 1: Figure S1. rfp editing identified by agarose gel electrophoresis and sequencing. Figure S2. pBBR1-Cas9-rfpF-rfpR clearance. Figure S3. Four genes edited by CRISPR-Cas9. Table S1. Putative restriction endonuclease genes in R. eutropha H16. Table S2. Genes related to putative NHEJ in R. eutropha. Table S3. List of plasmids used in this study. Table S4. List of main primers used in this study

Historical records and contamination assessment of potential toxic elements (PTEs) over the past 100 years in Ny-Ålesund, Svalbard

Ny-Ålesund has been significantly impacted by anthropogenic activities (e.g. coal mining, scientific research, tourist shipping) over the past 100 years. However, the studies of potential toxic elements (PTEs) contamination in Ny-Ålesund currently mainly focus on surface soil or surface fjord sediments, and little is known about the history and status of PTEs contamination over the past 100 years. In this study, we collected a palaeo-notch sediment profile YN, analyzed the contents of six typical PTEs (Cu, Pb, Cd, Hg, As, Se) in the sediments, and assessed the historical pollution status in Ny-Ålesund using the pollution load index, geo-accumulation index and enrichment factor. The results showed that the contents of PTEs over the past 100 years increased rapidly compared with those during the interval of 9400-100 BP. In addition, Pb, Cd and Hg showed a clear signal of enrichment and were the main polluters among the PTEs analyzed. The contamination was likely linked to gas-oil powered generators, coal mining, research station, tourist shipping and long-range transport of pollutants

MOESM2 of Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique

Additional file 2. Profile and sequence of rfp editing plasmid pBBR1-Cas9-rfpF-rfpR