Phosphoprotein Phosphatase 1 Is Required for Extracellular Calcium-Induced Keratinocyte Differentiation

Extracellular calcium is a major regulator of keratinocyte differentiation in vitro and appears to play that role in vivo, but the mechanism is unclear. We have previously demonstrated that, following calcium stimulation, PIP5K1α is recruited by the E-cadherin-β-catenin complex to the plasma membrane where it provides the substrate PIP2 for both PI3K and PLC-γ1. This signaling pathway is critical for calcium-induced generation of second messengers including IP3 and intracellular calcium and keratinocyte differentiation. In this study, we explored the upstream regulatory mechanism by which calcium activates PIP5K1α and the role of this activation in calcium-induced keratinocyte differentiation. We found that treatment of human keratinocytes in culture with calcium resulted in an increase in serine dephosphorylation and PIP5K1α activation. PP1 knockdown blocked extracellular calcium-induced increase in serine dephosphorylation and activity of PIP5K1α and induction of keratinocyte differentiation markers. Knockdown of PLC-γ1, the downstream effector of PIP5K1α, blocked upstream dephosphorylation and PIP5K1α activation induced by calcium. Coimmunoprecipitation revealed calcium induced recruitment of PP1 to the E-cadherin-catenin-PIP5K1α complex in the plasma membrane. These results indicate that PP1 is recruited to the extracellular calcium-dependent E-cadherin-catenin-PIP5K1α complex in the plasma membrane to activate PIP5K1α, which is required for PLC-γ1 activation leading to keratinocyte differentiation

Mudskipper genomes provide insights into the terrestrial adaptation of amphibious fishes

Mudskippers are amphibious fishes that have developed morphological and physiological adaptations to match their unique lifestyles. Here we perform whole-genome sequencing of four representative mudskippers to elucidate the molecular mechanisms underlying these adaptations. We discover an expansion of innate immune system genes in the mudskippers that may provide defence against terrestrial pathogens. Several genes of the ammonia excretion pathway in the gills have experienced positive selection, suggesting their important roles in mudskippers’ tolerance to environmental ammonia. Some vision-related genes are differentially lost or mutated, illustrating genomic changes associated with aerial vision. Transcriptomic analyses of mudskippers exposed to air highlight regulatory pathways that are up- or down-regulated in response to hypoxia. The present study provides a valuable resource for understanding the molecular mechanisms underlying water-to-land transition of vertebrates

A Single-Way Ranging Localization of AUVs Based on PSO of Outliers Elimination

Localization of autonomous underwater vehicles (AUVs) is a very important and challenging task for the AUVs applications. In long baseline underwater acoustic localization networks, the accuracy of single-way range measurements is the key factor for the precision of localization of AUVs, whether it is based on the way of time of arrival (TOA), time difference of arrival (TDOA), or angle of arrival (AOA). The single-way range measurements do not depend on water quality and can be taken from long distances; however, there are some limitations which exist in these measurements, such as the disturbance of the unknown current velocity and the outliers caused by sensors and errors of algorithm. To deal with these problems, an AUV self-localization algorithm based on particle swarm optimization (PSO) of outliers elimination is proposed, which improves the performance of angle of arrival (AOA) localization algorithm by taking account of effects of the current on the positioning accuracy and eliminating possible outliers during the localization process. Some simulation experiments are carried out to illustrate the performance of the proposed method compared with another localization algorithm

Investigation of Transient Characteristics of a Vertical Axial-Flow Pump with Non-Uniform Suction Flow

The aim of this paper is to study the influence of non-uniform suction flow on the transient characteristics of a vertical axial-flow pump device. The unsteady calculation is employed to forecast the unstable flow structure with three inlet deflection angles α, and the calculation accuracy under uniform inlet flow is verified by the external characteristic test. The results depict that a promotion in the α will increase the head and shaft power and thus improve the stress and fatigue failure risk of the impeller. At the impeller inlet, the pressure pulsation intensity (PPI) with α = 40° is lower than that with α = 0° caused by a decline in the axial velocity. The dominant frequency of the unsteady pressure signal is the blade-passing frequency (BPF), and the dominant frequency amplitude rises with the increase in α due to the improvement of the pre-rotation impact intensity. At the guide vanes inlet, the dominant frequency of the unsteady pressure signal at the guide vane inlet is also the blade-passing frequency. An improvement in α magnifies the angle between the trailing edge jet of the impeller and the leading edge of the guide vanes under 0.8Qdes and 1.0Qdes, while it diminishes the angle under 1.2Qdes. Thus, the PPI and dominant frequency amplitude with α = 40° are higher than that with α = 0° under 0.8Qdes and 1.0Qdes, but these are lower than that with α = 0° under 1.2Qdes

Investigation of Transient Characteristics of a Vertical Axial-Flow Pump with Non-Uniform Suction Flow

The aim of this paper is to study the influence of non-uniform suction flow on the transient characteristics of a vertical axial-flow pump device. The unsteady calculation is employed to forecast the unstable flow structure with three inlet deflection angles α, and the calculation accuracy under uniform inlet flow is verified by the external characteristic test. The results depict that a promotion in the α will increase the head and shaft power and thus improve the stress and fatigue failure risk of the impeller. At the impeller inlet, the pressure pulsation intensity (PPI) with α = 40° is lower than that with α = 0° caused by a decline in the axial velocity. The dominant frequency of the unsteady pressure signal is the blade-passing frequency (BPF), and the dominant frequency amplitude rises with the increase in α due to the improvement of the pre-rotation impact intensity. At the guide vanes inlet, the dominant frequency of the unsteady pressure signal at the guide vane inlet is also the blade-passing frequency. An improvement in α magnifies the angle between the trailing edge jet of the impeller and the leading edge of the guide vanes under 0.8Qdes and 1.0Qdes, while it diminishes the angle under 1.2Qdes. Thus, the PPI and dominant frequency amplitude with α = 40° are higher than that with α = 0° under 0.8Qdes and 1.0Qdes, but these are lower than that with α = 0° under 1.2Qdes

Peak-shaving balance of regional power grid based on sensitivity analysis of inter-provincial mutual aid

Pressure on the balance of power grid peak-shaving and the consumption of renewable energy is increasing year by year. If the peak-shaving balance problem of the power grid cannot be coordinated efficiently, it will lead to waste of resources such as abandon water and renewable energy. It is not difficult to conclude from historical data that the difficult moments of peak shaving in the regional power grids are usually not at the same period. For a given moment, if area A is lack of peak-shaving ability while area B has plenty of peak-shaving ability. We name the peak-shaving ability borrowed from area B to area A through the transmission line as inter-provincial mutual aid. The overall peak-shaving capacity of the regional power grid can be optimized through the inter-provincial mutual aid measures during the difficult peak-shaving period of each province. This will lead to the minimum abandon of renewable energy. Based on the conception of inter-provincial mutual aid, the authors studied the sensitivity of different inter-provincial mutual aid models to the impact of regional power grid peak shaving balance. Firstly, a method of obtaining the sensitivity of the regional power grid’s peak-shaving balance capability to the inter-provincial mutual aid capability is proposed. Then, the correctness of the raised method is verified through several examples of the Central China Power Grid. The last but not least, the above research results can be used to guide which transmission section has the first priority to be strengthened under a given investment scale

A Comprehensive Review on Energy Storage System Optimal Planning and Benefit Evaluation Methods in Smart Grids

Smart grids are the ultimate goal of power system development. With access to a high proportion of renewable energy, energy storage systems, with their energy transfer capacity, have become a key part of the smart grid construction process. This paper first summarizes the challenges brought by the high proportion of new energy generation to smart grids and reviews the classification of existing energy storage technologies in the smart grid environment and the practical application functions of energy storage in smart grids. Secondly, optimization planning and the benefit evaluation methods of energy storage technologies in the three different main application scenarios, including the grid side, user side, and new energy side, are analyzed. The advantages and shortcomings of the current research are also pointed out. Furthermore, the paper sheds light on the pressing issues that demand further consideration in energy storage planning. Finally, the aspects that warrant attention in the future application and promotion processes are elucidated in detail, culminating in a comprehensive understanding of the energy storage technologies in smart grids