Synergistic Effect of Trehalose and Saccharose Pretreatment on Maintenance of Lyophilized Human Red Blood Cell Quality

Purpose: To investigate the synergistic effect of trehalose and saccharose pretreatment on maintenance of lyophilized human red blood cell (RBC) quality.Methods: RBCs were pre-treated with trehalose and saccharose, and then lyophilized and re-hydrated. Prior to lyophilization and after re hydration, RBC parameters, RBC counts, total hemoglobin concentration, mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), comprehensive deformation index, hemolysis ratio and phosphatidylserine (PS) expression, were determined using a hematology analyzer, an RBC deformation instrument, a spectrophotometer and a flow cytometer, respectively. Superoxide dismutase (SOD), glucose-6-phosphate dehydrogenase (G-6-PD), and adenosine triphosphatase (ATPase) activities were determined using kits for SOD, ATPase, and G-6- PD assay, respectively.Results: After lyophilization-rehydration, RBC counts and total hemoglobin recovery rates, deformability, and RBC SOD, ATPase, and G-6-PD activities were significantly decreased by 47.24 – 74.65 % (p < 0.01), compared with the normal group. RBC osmotic fragility and PS expression on the outer surface of the RBC membrane were significantly increased by 168.53 and 629.30 % (p < 0.01), respectively, compared with the normal group. RBC MCH and MCV values were not significantly affected by lyophilization rehydration (p > 0.05). Trehalose and saccharose pretreatment significantly reversed the effects of lyophilization-rehydration on these RBC parameters by approximately 13.16 – 211.11 % (p < 0.01), compared with the control group. The combined effects were synergistic.Conclusion: Trehalose and saccharose pretreatment synergistically enhances maintenance of lyophilized RBC quality.Keywords: Trehalose, Saccharose, Lyophilization, Red blood cell, Hematological parameter

Poly[tetra­kis(μ4-4,6-dimethyl-5-nitro­benzene-1,3-dicarboxyl­ato-κ2 O 1:O 1′:O 3:O 3′)bis­(pyridine-κN)dizinc]

In the title complex, [Zn2(C10H7NO6)2(C5H5N)2]n, the repeat unit is a centrosymmetic tetra-carboxyl­ato-O,O’-bridged dimer in which each ZnII atom is five-coordinated by four O atoms from different dianionic 4,6-dimethyl-5-nitro­iso­phthalate ligands [Zn—O = 2.0283 (18)–2.0540 (19) Å] and one N atom from a pyridine mol­ecule [Zn—N = 2.030 (2) Å] in the axial site of a slightly distorted square-pyramidal coordination sphere. The Zn⋯Zn separation is 2.9750 (6) Å. The complex dimers are extended into a two-dimensional polymeric structure parallel to (100) through bridges provided by the second carboxyl­ate group of the ligand

A tetra­gonal polymorph of bis­[hydro­tris­(pyrazol-1-yl)borato]iron(II)

The title compound, [Fe(C9H10BN6)2], is a polymorph of a compound reported previously [Oliver et al. (1980 ▶). Inorg. Chem. 19, 165–168]. In the previous report, the compound crystallized in the monoclinic space group P21/c (Z = 4), whereas the crystal symmetry of the compound reported here is tetra­gonal (P42/ncm, Z = 4). The mol­ecular structure is comprised of two hydro­tris­(1-pyrazol­yl)borate ligands (Tp−) and a central FeII ion, which is coordinated by six pyrazole N atoms from two two Tp− ligands, yielding a distorted bipyramidal FeN6 geometry. The complete molecule exhibits symmetry 2/m

Hybrid CuCoO-GO enables ultrasensitive detection of antibiotics with enhanced laser desorption/ionization at nano-interfaces

The soaring concerns globally on antibiotic overuse have made calls for the development of rapid and sensitive detection methods urgent. Here we report that the hybrid CuCoO-GO matrix allows for sensitive detection of various antibiotics in combination with MALDI TOF MS. The new matrix is composed of few-layered GO nanosheets decorated with CuCoO nanoparticles with an average size of 10 nm, and exhibits excellent aqueous suspensibility. Accurate quantitation of the sulfonamide antibiotics in milk samples have been demonstrated using a CuCoO-GO matrix and a stable isotope (C13)-labeled analyte as the internal standard. Our experiments have achieved lower limits of detection (LOD) by several hundred fold for the detection of a panel of representative antibiotics, in comparison with the literature reports. Both intrabacterial and extrabacterial residual antibiotics can be sensitively detected with our method. We have further investigated the molecular mechanism of the enhanced desorption/ionization efficiency by the CuCoO-GO matrix with synchrotron radiation techniques for the first time. This work provides a sensitive matrix enabling MALDI-TOF MS to be applied in small molecular analysis, but also presents a distinct perspective on the mechanism behind the material functions

3-[(2-Hydroxy­ethyl)imino­meth­yl]-1,1′-bi-2-naphthol

In the title compound, C23H19NO3, there is an intra­molecular O—H⋯N hydrogen bond, which forms a six-membered ring, and inter­molecular O—H⋯O hydrogen bonds stabilize the crystal structure