Primer sequences for site-directed mutagenesis.

<p>Primer sequences for site-directed mutagenesis.</p

Expression of the human CYP2D6 reporter constructs in the human hepatoma cell line (HepG2).

<p>The relative luciferase activities were normalized against the human wild type construct. The results are the average of at least three independent experiments. Comparisons among groups were done with the aid of the ANOVA statistical procedure. *Significantly different from Wild type transfected cells (P<0.05). **Significantly different from Wild type transfected cells (P<0.01), ***Significantly different from Wild type transfected cells (P<0.001).</p

Selected haplotypes of the <i>CYP2D6</i> regulatory region in the Chinese Han population.

<p>Selected haplotypes of the <i>CYP2D6</i> regulatory region in the Chinese Han population.</p

Predictive analysis (performed by CONREAL and ConSite) of transcription factor binding sites affected by potential regulatory SNPs in <i>CYP2D6</i>.

<p>There is no change of transcription factors for −1589G>C.</p

Image1_VitroGel-loaded human MenSCs promote endometrial regeneration and fertility restoration.JPEG

Introduction: Intrauterine adhesions (IUA), also known as Asherman's syndrome, is caused by trauma to the pregnant or non-pregnant uterus, which leads to damaged endometrial basal lining and partial or total occlusion of the uterine chambers, resulting in abnormal menstruation, infertility, or recurrent miscarriage. The essence of this syndrome is endometrial fibrosis. And there is no effective treatment for IUA to stimulate endometrial regeneration currently. Recently, menstrual blood-derived stem cells (MenSCs) have been proved to hold therapeutic promise in various diseases, such as myocardial infarction, stroke, diabetes, and liver cirrhosis.Methods: In this study, we examined the effects of MenSCs on the repair of uterine adhesions in a rat model, and more importantly, promoted such therapeutic effects via a xeno-free VitroGel MMP carrier.Results: This combined treatment reduced the expression of inflammatory factors, increased the expression of anti-inflammatory factors, restricted the area of endometrial fibrosis, diminished uterine adhesions, and partially restored fertility, showing stronger effectiveness than each component alone and almost resembling the sham group.Discussion: Our findings suggest a highly promising strategy for IUA treatment.</p

Flowchart of dynamic transcriptional regulatory network construction.

<p>Flowchart of dynamic transcriptional regulatory network construction.</p

Dynamic of pathway-crosstalk.

<p>The red line indicated the p-value of cross-talking between two pathways less than 0.1. The blue line indicated the cross-talking was not significant with the p-value large than 0.3.</p

Significant pathways.

<p>Significant pathways.</p

Hierarchical clustering in the context of a defined regulatory network.

<p>(A) The adjusted strength matrix was used for clustering, with the gene expression matrix appended. Four major clusters, which have more than three associated genes, were highlighted. In the adjusted strength matrix heatmap, white indicated a weak regulatory influence. (B) Clustering with gene expression.</p

A dynamic network of transcription in response to MGd.

<p>Target genes were noted with circles, and TFs with triangle. The 4 subcellular locations (Nucleus; Cytoplasm; Plasma membrane; Extracelluar) were grouped in 4 cycles. Green dotted lines denoted of a target gene which may transfer between two subcellular locations. Red and blue lines showed the influence of a transcription factor on a target gene.</p