1 research outputs found
Comprehensive Analysis of Acylcarnitine Species in <i>db/db</i> Mouse Using a Novel Method of High-Resolution Parallel Reaction Monitoring Reveals Widespread Metabolic Dysfunction Induced by Diabetes
Acylcarnitines are
exerting a variety of biological functions depending
on the differences in lengths, saturation levels, and conjugation
groups, which to a great extent contribute to the challenges of acylcarnitines
quantifications due to various kinds of isomers. Here, we describe
a novel method by using high-resolution parallel reaction monitoring
(PRM) liquid chromatography-tandem mass spectrometry (LC-MS/MS). Both
reversed-phase and normal-phase column were used in order to get accurate,
reliable, widespread quantification of acylcarnitines, and without
tedious sample preparation procedure. The method provided the most
comprehensive acylcarnitine profile with high-resolution MS and MS/MS
confirmation to date. A total of 117 acylcarnitines were detected
from plasma and urine samples. The application of targeted profiling
of acylcarnitines in <i>db/m+</i> control and <i>db/db</i> diabetic mice indicated incomplete amino acid and fatty acid oxidation
on diabetic mice. Interestingly, the reduction of medium odd-numbered
chain acylcarnitines in urine samples was first observed between <i>db/m+</i> and <i>db/db</i> mice. The high-resolution
PRM method makes it possible to monitor the widespread metabolic changes
of the acylcarnitines in response to stimuli. Besides, the accurate
MS and MS/MS spectra data of the 117 acylcarnitines could be used
as mass spectrometric resources for the identification of acylcarnitines