23 research outputs found
Media 1: Principle and characteristics of 3D display based on random source constructive interference
Originally published in Optics Express on 14 July 2014 (oe-22-14-16863
Chemiluminescent Labels Released from Long Spacer Arm-Functionalized Magnetic Particles: A Novel Strategy for Ultrasensitive and Highly Selective Detection of Pathogen Infections
Previously, the unique advantages
provided by chemiluminescence
(CL) and magnetic particles (MPs) have resulted in the development
of many useful nucleic acid detection methods. CL is highly sensitive,
but when applied to MPs, its intensity is limited by the inner filter-like
effect arising from excess dark MPs. Herein, we describe a modified
strategy whereby CL labels are released from MPs to eliminate this
negative effect. This approach relies on (1) the magnetic capture
of target molecules on long spacer arm-functionalized magnetic particles
(LSA-MPs), (2) the conjugation of streptavidin-alkaline phosphatase
(SA-AP) to biotinylated amplicons of target pathogens, (3) the release
of CL labels (specifically, AP tags), and (4) the detection of the
released labels. CL labels were released from LSA-MPs through LSA
ultrasonication or DNA enzymolysis, which proved to be the superior
method. In contrast to conventional MPs, LSA-MPs exhibited significantly
improved CL detection, because of the introduction of LSA, which was
made of water-soluble carboxymethylated β-1,3-glucan. Detection
of hepatitis B virus with this technique revealed a low detection
limit of 50 fM, high selectivity, and excellent reproducibility. Thus,
this approach may hold great potential for early stage clinical diagnosis
of infectious diseases
Catalytic Enantioselective Nucleophilic α‑Chlorination of Ketones with NaCl
Catalytic
enantioselective α-chlorination of ketones
is a
highly desirable process. Different from the conventional approaches
that employ corrosive electrophilic chlorination reagents, the process
disclosed here employs nucleophilic chloride, aqueous NaCl solution,
and even seawater, as green inexpensive chlorine sources. This mechanistically
distinct and electronically opposite approach provides facile access
to diverse highly enantioenriched acyclic α-chloro ketones that
are less straightforward by conventional approaches. With a chiral
thiourea catalyst, a range of racemic α-keto sulfonium salts
underwent enantioconvergent carbon–chlorine bond formation
with high efficiency and excellent enantioselectivity under mild conditions.
The sulfonium motif plays a crucial triple role by permitting smooth
dynamic kinetic resolution to take place via a chiral anion binding
mechanism in a well-designed phase-transfer system. This protocol
represents a new general platform for the asymmetric nucleophilic
α-functionalization of carbonyl compounds
Additional file 1 of DNMT3A mutation promotes leukemia development through NAM-NAD metabolic reprogramming
Additional file 1: Table S1. Primer sequences used in Q-RT-PCR
Additional file 2 of DNMT3A mutation promotes leukemia development through NAM-NAD metabolic reprogramming
Additional file 2: Table S2. The mRNA expression level of NAMPT in AML obtained from the Oncomine database
Selection of HBsAg-Specific DNA Aptamers Based on Carboxylated Magnetic Nanoparticles and Their Application in the Rapid and Simple Detection of Hepatitis B Virus Infection
Aptamers
are short single-stranded DNA or RNA oligonucleotides and can be selected
from synthetic combinatorial libraries in vitro. They have a high
binding affinity and specificity for their targets. Agarose gels,
nitrocellulose membranes, and adsorptive microplates are often used
as carriers to immobilize targets in the SELEX (systematic evolution
of ligands by exponential enrichment) process, but the subsequent
separation step is tedious and time-consuming. Therefore, we used
magnetic nanoparticles (MNPs) as carriers to immobilize the target,
hepatitis B surface antigen (HBsAg), which is convenient for fast
magnetic separation. In this study, we first selected DNA aptamers
against HBsAg by immobilizing HBsAg on the surface of carboxylated
MNPs. The ssDNA library of each selection round was prepared by asymmetric
PCR amplification for the next selection round. To obtain aptamer
sequences, the final selected products were purified by gel electrophoresis,
then cloned, and sequenced. DNA aptamers that specifically bind to
HBsAg were successfully obtained after 13 selection rounds. The selected
aptamers were used to construct a chemiluminescence aptasensor based
on magnetic separation and immunoassay to detect HBsAg from pure protein
or actual serum samples. There was a linear relationship between HBsAg
concentration and chemiluminescent intensity in the range of 1–200
ng/mL. The aptasensor worked well even in the presence of interfering
substances and was highly specific in the detection of HBsAg in serum
samples, with a detection limit 0.1 ng/mL lower than the 0.5 ng/mL
limit of an ELISA in use at the hospital. This aptasensor can contribute
to better detection of hepatitis B virus infection
Image_4_The efficacy of cognitive behavioral therapy for cancer: A scientometric analysis.TIF
Cognitive behavioral therapy (CBT) is one of the most recognized psychological interventions to improve the overall quality of life of cancer survivors. To analyze current research trends in the field of the link between CBT and cancer and to provide potential future research directions, we conducted the scientometric analysis. The study was conducted on all documents in this field from 2012 to 2022 retrieved from Web of Science. Then Biblioshiny, VOSviewer software, and CiteSpace software were used for getting the information of article postings and citations, countries, institutions, journals, authors, and keywords. The number of documents about the link between CBT and cancer from 2012 to 19 July 2022, was 619, with 476 of articles and 143 of reviews. The number of annual publications has been fluctuating, with the highest number of publications in 2020. The country with the maximum number of publications and citations was the US. The University of Houston was the organization with the highest quantity of publications and total link strength (TLS). Psycho-Oncology was the most active journal in the field and has the highest h-index. Zvolensky MJ was the author with the highest quantity of publications. The most cited keywords were “Quality-of-life,” “Cognitive-behavioral therapy,” “Depression,” “Cognitive therapy” and “Breast-cancer.” And as evidenced by the keyword citations, the focus of this research area has gradually shifted to the mental health of patients and the underlying pathogenesis. The impact of CBT in cancer treatment is now well established and has gradually evolved toward symptom-specific treatment. However, the relationship between CBT and cancer has not been further developed. Future research is needed to be further developed in the identification of a generic formula for CBT in cancer and the exploration of mechanisms of CBT and cancer.</p
Image_1_The efficacy of cognitive behavioral therapy for cancer: A scientometric analysis.TIF
Cognitive behavioral therapy (CBT) is one of the most recognized psychological interventions to improve the overall quality of life of cancer survivors. To analyze current research trends in the field of the link between CBT and cancer and to provide potential future research directions, we conducted the scientometric analysis. The study was conducted on all documents in this field from 2012 to 2022 retrieved from Web of Science. Then Biblioshiny, VOSviewer software, and CiteSpace software were used for getting the information of article postings and citations, countries, institutions, journals, authors, and keywords. The number of documents about the link between CBT and cancer from 2012 to 19 July 2022, was 619, with 476 of articles and 143 of reviews. The number of annual publications has been fluctuating, with the highest number of publications in 2020. The country with the maximum number of publications and citations was the US. The University of Houston was the organization with the highest quantity of publications and total link strength (TLS). Psycho-Oncology was the most active journal in the field and has the highest h-index. Zvolensky MJ was the author with the highest quantity of publications. The most cited keywords were “Quality-of-life,” “Cognitive-behavioral therapy,” “Depression,” “Cognitive therapy” and “Breast-cancer.” And as evidenced by the keyword citations, the focus of this research area has gradually shifted to the mental health of patients and the underlying pathogenesis. The impact of CBT in cancer treatment is now well established and has gradually evolved toward symptom-specific treatment. However, the relationship between CBT and cancer has not been further developed. Future research is needed to be further developed in the identification of a generic formula for CBT in cancer and the exploration of mechanisms of CBT and cancer.</p
Table_1_The efficacy of cognitive behavioral therapy for cancer: A scientometric analysis.docx
Cognitive behavioral therapy (CBT) is one of the most recognized psychological interventions to improve the overall quality of life of cancer survivors. To analyze current research trends in the field of the link between CBT and cancer and to provide potential future research directions, we conducted the scientometric analysis. The study was conducted on all documents in this field from 2012 to 2022 retrieved from Web of Science. Then Biblioshiny, VOSviewer software, and CiteSpace software were used for getting the information of article postings and citations, countries, institutions, journals, authors, and keywords. The number of documents about the link between CBT and cancer from 2012 to 19 July 2022, was 619, with 476 of articles and 143 of reviews. The number of annual publications has been fluctuating, with the highest number of publications in 2020. The country with the maximum number of publications and citations was the US. The University of Houston was the organization with the highest quantity of publications and total link strength (TLS). Psycho-Oncology was the most active journal in the field and has the highest h-index. Zvolensky MJ was the author with the highest quantity of publications. The most cited keywords were “Quality-of-life,” “Cognitive-behavioral therapy,” “Depression,” “Cognitive therapy” and “Breast-cancer.” And as evidenced by the keyword citations, the focus of this research area has gradually shifted to the mental health of patients and the underlying pathogenesis. The impact of CBT in cancer treatment is now well established and has gradually evolved toward symptom-specific treatment. However, the relationship between CBT and cancer has not been further developed. Future research is needed to be further developed in the identification of a generic formula for CBT in cancer and the exploration of mechanisms of CBT and cancer.</p
Image_3_The efficacy of cognitive behavioral therapy for cancer: A scientometric analysis.TIF
Cognitive behavioral therapy (CBT) is one of the most recognized psychological interventions to improve the overall quality of life of cancer survivors. To analyze current research trends in the field of the link between CBT and cancer and to provide potential future research directions, we conducted the scientometric analysis. The study was conducted on all documents in this field from 2012 to 2022 retrieved from Web of Science. Then Biblioshiny, VOSviewer software, and CiteSpace software were used for getting the information of article postings and citations, countries, institutions, journals, authors, and keywords. The number of documents about the link between CBT and cancer from 2012 to 19 July 2022, was 619, with 476 of articles and 143 of reviews. The number of annual publications has been fluctuating, with the highest number of publications in 2020. The country with the maximum number of publications and citations was the US. The University of Houston was the organization with the highest quantity of publications and total link strength (TLS). Psycho-Oncology was the most active journal in the field and has the highest h-index. Zvolensky MJ was the author with the highest quantity of publications. The most cited keywords were “Quality-of-life,” “Cognitive-behavioral therapy,” “Depression,” “Cognitive therapy” and “Breast-cancer.” And as evidenced by the keyword citations, the focus of this research area has gradually shifted to the mental health of patients and the underlying pathogenesis. The impact of CBT in cancer treatment is now well established and has gradually evolved toward symptom-specific treatment. However, the relationship between CBT and cancer has not been further developed. Future research is needed to be further developed in the identification of a generic formula for CBT in cancer and the exploration of mechanisms of CBT and cancer.</p