PC-3 cells were cultured in serum-free medium for 24 h and then treated with or without cycloheximide (CHX, 5 μg/ml) for 1 hour prior to zinc (15 μM) exposure for 6 hours

<p><b>Copyright information:</b></p><p>Taken from "The Involvement of Bax in Zinc-Induced Mitochondrial Apoptogenesis in Malignant Prostate Cells"</p><p>http://www.molecular-cancer.com/content/7/1/25</p><p>Molecular Cancer 2008;7():25-25.</p><p>Published online 10 Mar 2008</p><p>PMCID:PMC2329666.</p><p></p

The cells were treated with medium supplemented with 15 μM zinc for 180 minutes versus no zinc treatment

<p><b>Copyright information:</b></p><p>Taken from "The Involvement of Bax in Zinc-Induced Mitochondrial Apoptogenesis in Malignant Prostate Cells"</p><p>http://www.molecular-cancer.com/content/7/1/25</p><p>Molecular Cancer 2008;7():25-25.</p><p>Published online 10 Mar 2008</p><p>PMCID:PMC2329666.</p><p></p

PC-3 cells were transfected with Bax siRNA or with empty vector and cultured for 72 hours; followed by treatment of the cells with 15 μM zinc for 24 hours

<p><b>Copyright information:</b></p><p>Taken from "The Involvement of Bax in Zinc-Induced Mitochondrial Apoptogenesis in Malignant Prostate Cells"</p><p>http://www.molecular-cancer.com/content/7/1/25</p><p>Molecular Cancer 2008;7():25-25.</p><p>Published online 10 Mar 2008</p><p>PMCID:PMC2329666.</p><p></p

The isolated mitochondria were exposed to medium supplemented with 15 μM zinc for 15 minutes or with unsupplemented medium (control)

<p><b>Copyright information:</b></p><p>Taken from "The Involvement of Bax in Zinc-Induced Mitochondrial Apoptogenesis in Malignant Prostate Cells"</p><p>http://www.molecular-cancer.com/content/7/1/25</p><p>Molecular Cancer 2008;7():25-25.</p><p>Published online 10 Mar 2008</p><p>PMCID:PMC2329666.</p><p></p

Presentation_3_Detection of African swine fever virus antibodies in serum using a pB602L protein-based indirect ELISA.pptx

African Swine Fever (ASF) is an acute, highly contagious and deadly infectious disease that has a huge impact on the swine industry. It is caused by the African swine fever virus (ASFV). The most acute forms of ASF in domestic pigs have mortality rates of up to 100%. The lack of a commercial vaccine and effective therapeutic drugs has brought great challenges to the prevention and control of ASF. Current, the African swine fever virus requires a huge amount of detection, so there is a need for more sensitive and accurate detection technology. The protein pB602L, as a late non-structural protein, has a high corresponding antibody titer and strong antigenicity in infected swine. In this research, the B602L gene was constructed into the pColdI prokaryotic expression vector, and prokaryotic expression of the soluble pB602L protein was induced by IPTG. Western blot analysis demonstrated that the protein had strong immunogenicity. We established an indirect ELISA method for the detection of anti-ASFV using purified recombinant pB602L protein as antigen. The detection method showed excellent specificity without cross-reactions with antibodies against PRRSV, CSFV, JEV, and GETV. The method could detect anti-ASFV in serum samples that were diluted up to 6,400 times, showing high sensitivity. The coefficients of variation of the intra-assay and inter-assay were both <10%. The assays had excellent specificity, sensitivity, and repeatability. In summary, we developed an accurate, rapid, and economical method for the detection of anti-ASFV in pig serum samples with great potential for ASF monitoring and epidemic control.</p

Presentation_2_Detection of African swine fever virus antibodies in serum using a pB602L protein-based indirect ELISA.pptx

African Swine Fever (ASF) is an acute, highly contagious and deadly infectious disease that has a huge impact on the swine industry. It is caused by the African swine fever virus (ASFV). The most acute forms of ASF in domestic pigs have mortality rates of up to 100%. The lack of a commercial vaccine and effective therapeutic drugs has brought great challenges to the prevention and control of ASF. Current, the African swine fever virus requires a huge amount of detection, so there is a need for more sensitive and accurate detection technology. The protein pB602L, as a late non-structural protein, has a high corresponding antibody titer and strong antigenicity in infected swine. In this research, the B602L gene was constructed into the pColdI prokaryotic expression vector, and prokaryotic expression of the soluble pB602L protein was induced by IPTG. Western blot analysis demonstrated that the protein had strong immunogenicity. We established an indirect ELISA method for the detection of anti-ASFV using purified recombinant pB602L protein as antigen. The detection method showed excellent specificity without cross-reactions with antibodies against PRRSV, CSFV, JEV, and GETV. The method could detect anti-ASFV in serum samples that were diluted up to 6,400 times, showing high sensitivity. The coefficients of variation of the intra-assay and inter-assay were both <10%. The assays had excellent specificity, sensitivity, and repeatability. In summary, we developed an accurate, rapid, and economical method for the detection of anti-ASFV in pig serum samples with great potential for ASF monitoring and epidemic control.</p

Presentation_1_Detection of African swine fever virus antibodies in serum using a pB602L protein-based indirect ELISA.pptx

African Swine Fever (ASF) is an acute, highly contagious and deadly infectious disease that has a huge impact on the swine industry. It is caused by the African swine fever virus (ASFV). The most acute forms of ASF in domestic pigs have mortality rates of up to 100%. The lack of a commercial vaccine and effective therapeutic drugs has brought great challenges to the prevention and control of ASF. Current, the African swine fever virus requires a huge amount of detection, so there is a need for more sensitive and accurate detection technology. The protein pB602L, as a late non-structural protein, has a high corresponding antibody titer and strong antigenicity in infected swine. In this research, the B602L gene was constructed into the pColdI prokaryotic expression vector, and prokaryotic expression of the soluble pB602L protein was induced by IPTG. Western blot analysis demonstrated that the protein had strong immunogenicity. We established an indirect ELISA method for the detection of anti-ASFV using purified recombinant pB602L protein as antigen. The detection method showed excellent specificity without cross-reactions with antibodies against PRRSV, CSFV, JEV, and GETV. The method could detect anti-ASFV in serum samples that were diluted up to 6,400 times, showing high sensitivity. The coefficients of variation of the intra-assay and inter-assay were both <10%. The assays had excellent specificity, sensitivity, and repeatability. In summary, we developed an accurate, rapid, and economical method for the detection of anti-ASFV in pig serum samples with great potential for ASF monitoring and epidemic control.</p